富含多酚的马齿苋(马齿苋)通过修复肠道屏障、肠道微生物群和代谢产物来缓解溃疡性结肠炎

IF 9.8 1区 农林科学 Q1 CHEMISTRY, APPLIED
Zheng Li , Tianjiao Chu , Xin Sun , Shen Zhuang , Dianbo Hou , Zhaohan Zhang , Jialu Sun , Yuhong Liu , Jing Li , Yifei Bian
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引用次数: 0

摘要

溃疡性结肠炎(UC)是一种由多种因素引起的复发性肠道疾病,目前长期使用治疗药物存在严重的不良反应和耐受性问题。开发用于UC治疗的天然食物来源和多靶向药物迫在眉睫。马齿苋作为一种蔬菜,在研究中已被证明具有抗uc的作用。然而,马齿苋丰富的有效成分与改善肠道屏障、肠道菌群和代谢产物之间的关系尚不清楚。本研究发现马齿苋(Portulaca oleracea L.)富含酚酸类活性成分,可有效缓解葡聚糖硫酸钠(DSS)诱导小鼠的体重减轻、疾病活动指数(DAI)评分和结肠长度。它还降低了CRP和MPO反应,降低了fitc -葡聚糖、LPS和EB的渗透,提高了组织病理学评分。同时,体外和体内验证表明马马苋对肠道屏障指标ZO-1、Occludin和Claudin-1具有保护作用,并通过NF-κB信号通路抑制炎症相关iNOS和NLRP3蛋白的表达。此外,马齿苋增加了肠道菌群的多样性,提高了丁酸梭菌属、多雷氏菌属和双歧杆菌属的比例,降低了拟杆菌属、Turicibacter和拟副杆菌属的比例。血清代谢组学分析显示,PO部署后39种代谢物的失衡得到显著逆转。富集分析表明,戊糖磷酸途径和丙酮酸代谢途径是PO抗UC的关键途径。综上所述,马齿苋通过抑制NF-κB信号通路,有效改善肠道屏障破坏和肠道炎症,调节肠道菌群和代谢物紊乱,发挥抗uc作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Polyphenols-rich Portulaca oleracea L. (purslane) alleviates ulcerative colitis through restiring the intestinal barrier, gut microbiota and metabolites
Ulcerative colitis (UC) is a recurrent intestinal disease caused by a complex of factors, and there are serious adverse effects and tolerance problems associated with the current long-term use of therapeutic drugs. The development of natural food sources and multi-targeted drugs for the treatment of UC is imminent. Portulaca oleracea L. (PO), as a vegetable, has been shown in studies to have an anti-UC effects. However, the relationship between the abundant active ingredients contained in Portulaca oleracea L. and the improvement of intestinal barrier, gut microbiota and metabolites is unclear. In the present study, Portulaca oleracea L. which was found to be rich in phenolic acid-based active ingredients, were effective in alleviating dextran sulfate sodium (DSS)-induced body weight loss, disease activity index (DAI) score and colon length in mice. It also decreased C-reactive protein (CRP) and myeloperoxidase (MPO) responses, reduced the permeation of fluorescein isothiocyanate (FITC)-dextran, lipopolysaccharide (LPS) and evans blue (EB), and improved histopathological scores. Meanwhile, in vitro and in vivo validation revealed the protective effects of purslane on the intestinal barrier indicators ZO-1, Occludin and Claudin-1, and inhibited the expression of inflammation-associated iNOS and NLRP3 proteins through the NF-κB signaling pathway. In addition, purslane increased the diversity of the intestinal flora, enhancing the proportion of the genera Butyricoccus, Dorea and Bifidobacterium and decreasing the percentage of Bacteroides, Turicibacter and Parabacteroides. Serum metabolomics analysis showed that the imbalance of 39 metabolites was significantly reversed after PO deployment. Enrichment analysis showed that Pentose phosphate pathway and Pyruvate metabolism pathway were the key pathways of PO against UC. Overall, purslane effectively improved the intestinal barrier disruption and intestinal inflammation by inhibiting the NF-κB signaling pathway, and adjusted the disorder of gut microbiota and metabolites to exert anti-UC effects.
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来源期刊
Food Chemistry
Food Chemistry 工程技术-食品科技
CiteScore
16.30
自引率
10.20%
发文量
3130
审稿时长
122 days
期刊介绍: Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.
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