超高效液相色谱-串联质谱法同时定量测定菜籽中14种硫代葡萄糖苷类化合物

IF 9.8 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2024-12-02 DOI:10.1016/j.foodchem.2024.142302

Meiyi Yao , Shige Xing , Guihong Yao , Wei Yong , Yun Ling , Baijun Chu

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引用次数: 0

摘要

硫代葡萄糖苷的种类和含量是十字花科植物及其产品的重要指标。然而，十字花科植物内源酶能水解硫代葡萄糖苷，影响了其定量检测。本研究以油菜科重要油料作物油菜籽为研究对象，建立了采用酶抑制预处理的高效液相色谱-质谱联用法测定硫代葡萄糖苷的新方法。这样可以在室温下测定完整的硫代葡萄糖苷。简单的过程包括用饱和氯化钠和甲醇均质样品，然后是涡流提取、离心和过滤。该方法的检出限和检出限分别为0.1 ~ 1.1 mg/kg和0.3 ~ 3.7 mg/kg，线性关系良好（R2 >； 0.990）。日内精密度（7个重复）为3.21 ~ 10.47 %，日内精密度（3 天）为3.13 ~ 15.97 %。

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Simultaneous quantification of 14 glucosinolates in rapeseeds by ultra high performance liquid chromatography – Tandem mass spectrometry

Glucosinolates' species and content serve as crucial indicators for Brassicaceae plants and their products. However, endogenous enzymes in Brassicaceae can hydrolyze glucosinolates, influencing their quantitative detection. Our study utilized rapeseed, a vital Brassicaceae oil crop, to establish a novel UHPLC-MS/MS method for glucosinolate determination, incorporating a pretreatment approach with enzyme inhibition. This enabled intact glucosinolate determination at room temperature. The straightforward process involved homogenizing samples with saturated sodium chloride and methanol, followed by vortex extraction, centrifugation, and filtration. The method exhibited a strong linear relationship (R² > 0.990), with LODs and LOQs ranging from 0.1 to 1.1 mg/kg and 0.3–3.7 mg/kg, respectively. Intra-day precision (7 repetitions) ranged from 3.21 to 10.47 %, while inter-day precision (3 days) ranged from 3.13 to 15.97 %.

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.