Melia azedarach 果实中的化学成分及其抗炎活性。

IF 6 2区医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Antioxidants Pub Date : 2024-10-31 DOI:10.3390/antiox13111338

Fan Cao, Jing Chen, Zheng-Tao Lin, Han-Ying Lin, Bin Liu, Zhen-Wei Chen, Xin-Hua Ma, Yong-Hong Zhang

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引用次数: 0

摘要

通过对 Melia azedarach 果实进行植物化学研究，分离出了一种新的三萜类 tirucallane (1)、四种新的柠檬酸类化合物 (2-5) 和四种已知的柠檬酸类化合物 (6-9)。通过全面的光谱和分光分析，这些化合物的结构得以明确。对分离出的化合物进行了体外抗炎活性评估。化合物 2 的抗炎效果最强，其 IC50 值为 22.04 μM。此外，化合物 2 还减少了 LPS 诱导的活性氧（ROS）的产生，并降低了炎症介质 IL-6 和 TNF-α 的水平。一项机理研究显示，类柠檬素 2 能抑制 iNOS 和 JAK2 的表达，并参与 NF-κB 信号级联的调节，从而揭示了它的抗炎作用。

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Chemical Constituents from the Fruit of Melia azedarach and Their Anti-Inflammatory Activity.

Phytochemical investigations of Melia azedarach fruits have led to the isolation of a novel tirucallane triterpenoid (1), four new limonoids (2-5), and four known limonoids (6-9). Their structures were clarified by comprehensive spectroscopic and spectrometric analyses. The anti-inflammatory activities of isolated compounds were assessed in vitro. Compound 2 exhibited the most potent anti-inflammatory effect, with an IC₅₀ value of 22.04 μM. Additionally, compound 2 attenuated LPS-induced reactive oxygen species (ROS) production and reduced the levels of inflammatory mediators IL-6 and TNF-α. A mechanistic study revealed that limonoid 2 suppresses the expression of iNOS and JAK2 and is implicated in the modulation of the NF-κB signaling cascade, which reveals its anti-inflammatory actions.

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来源期刊

Antioxidants Biochemistry, Genetics and Molecular Biology-Physiology

CiteScore

10.60

自引率

11.40%

发文量

2123

审稿时长

16.3 days

期刊介绍： Antioxidants (ISSN 2076-3921), provides an advanced forum for studies related to the science and technology of antioxidants. It publishes research papers, reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.