基于转录组的紫外线-B 强化条件下芒果果肉中谷胱甘肽和抗坏血酸的积累。

IF 6 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Hassam Tahir, Muhammad Sajjad, Minjie Qian, Muhammad Zeeshan Ul Haq, Ashar Tahir, Muhammad Aamir Farooq, Ling Wei, Shaopu Shi, Kaibing Zhou, Quansheng Yao
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引用次数: 0

摘要

芒果(Mangifera indica)是一种营养丰富的热带水果,受紫外线-B 辐射的影响很大,紫外线-B 辐射会诱发氧化应激,破坏生理过程。本研究旨在调查芒果果肉对紫外线-B 胁迫(96 kJ/mol)的分子和生化反应,从未成熟阶段到成熟阶段,连续三年每隔 10 天采集一次样本。紫外线-B 胁迫既影响非酶参数,如成熟指数、活性氧(ROS)水平、膜渗透性,也影响抗坏血酸-谷胱甘肽(AsA-GSH)循环的关键酶成分。这些酶包括谷胱甘肽还原酶(GR)、γ-谷氨酰转移酶(GGT)、谷胱甘肽 S-转移酶(GST)、谷胱甘肽过氧化物酶(GPX)、葡萄糖-6-磷酸脱氢酶(GPX)、抗坏血酸-谷胱甘肽(AsA-GSH)循环、葡萄糖-6-磷酸脱氢酶(G6PDH)、半乳糖-1,4-内酯脱氢酶(GalLDH)、抗坏血酸过氧化物酶(APX)、抗坏血酸氧化酶(AAO)和单脱氢抗坏血酸还原酶(MDHAR)。转录组分析发现了 18 个与 AsA-GSH 循环相关的差异表达基因(DEGs),包括 MiGR、MiGGT1、MiGGT2、MiGPX1、通过 qRT-PCR 验证,这些基因包括 MiGR、MiGGT1、MiGGT2、MiGPX1、MiGPX2、MiGST1、MiGST2、MiGST3、MiG6PDH1、MiG6PDH2、MiGalLDH、MiAPX1、MiAPX2、MiAAO1、MiAAO2、MiAAO3、MiAAO4 和 MiMDHAR。研究结果表明,紫外线-B 胁迫激活了芒果果肉中复杂的调控网络,以优化 ROS 解毒和保护抗氧化剂,为提高热带果树抵御环境胁迫的能力提供了启示。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Glutathione and Ascorbic Acid Accumulation in Mango Pulp Under Enhanced UV-B Based on Transcriptome.

Mango (Mangifera indica), a nutritionally rich tropical fruit, is significantly impacted by UV-B radiation, which induces oxidative stress and disrupts physiological processes. This study aimed to investigate mango pulp's molecular and biochemical responses to UV-B stress (96 kJ/mol) from the unripe to mature stages over three consecutive years, with samples collected at 10-day intervals. UV-B stress affected both non-enzymatic parameters, such as maturity index, reactive oxygen species (ROS) levels, membrane permeability, and key enzymatic components of the ascorbate-glutathione (AsA-GSH) cycle. These enzymes included glutathione reductase (GR), gamma-glutamyl transferase (GGT), glutathione S-transferases (GST), glutathione peroxidase (GPX), glucose-6-phosphate dehydrogenase (G6PDH), galactono-1,4-lactone dehydrogenase (GalLDH), ascorbate peroxidase (APX), ascorbate oxidase (AAO), and monodehydroascorbate reductase (MDHAR). Transcriptomic analysis revealed 18 differentially expressed genes (DEGs) related to the AsA-GSH cycle, including MiGR, MiGGT1, MiGGT2, MiGPX1, MiGPX2, MiGST1, MiGST2, MiGST3, MiG6PDH1, MiG6PDH2, MiGalLDH, MiAPX1, MiAPX2, MiAAO1, MiAAO2, MiAAO3, MiAAO4, and MiMDHAR, validated through qRT-PCR. The findings suggest that UV-B stress activates a complex regulatory network in mango pulp to optimize ROS detoxification and conserve antioxidants, offering insights for enhancing the resilience of tropical fruit trees to environmental stressors.

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来源期刊
Antioxidants
Antioxidants Biochemistry, Genetics and Molecular Biology-Physiology
CiteScore
10.60
自引率
11.40%
发文量
2123
审稿时长
16.3 days
期刊介绍: Antioxidants (ISSN 2076-3921), provides an advanced forum for studies related to the science and technology of antioxidants. It publishes research papers, reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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