基于磁小体免疫磁分离结合量子点免疫测定技术的快速灵敏夹心法检测苹果汁中的酸根尖螺旋体

IF 8.5 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2024-11-26 DOI:10.1016/j.foodchem.2024.142234

Jianrui Qi , Kai Wang , Xiaohai Yan , Xinru Dong , Rui Sun , Yahong Yuan , Tianli Yue

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引用次数: 0

摘要

苹果汁受到Alicyclobacillus acidoterrestris（A. acidoterrestris）的污染会造成重大经济损失。因此，有必要开发一种快速、灵敏的方法来检测 A. acidoterrestris。针对这一问题，本研究制备了磁性体-单克隆抗体免疫磁性微球（IMMs）作为捕获探针，以富集 A. acidoterrestris（IMMs-Aa）；制备了单克隆抗体-量子点（mAb-CdTeQDs）作为检测探针，以检测 A. acidoterrestris。获得的 IMMs-Aa-mAb-CdTeQDs "三明治 "结构用于根据荧光强度检测 A. acidoterrestris。该策略在荧光强度和 A. acidoterrestris 的浓度（101-105 CFU/mL）之间呈现出良好的线性相关性（y = 1048× + 1891，R2 = 0.995），50 分钟内的检测限低至 25.4 CFU/mL。该方法在苹果汁中的回收率为 88.16 % 至 107.03 %。因此，本研究建立了一种高效、高灵敏度的磁捕获-富集-分离-检测方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

A rapid and sensitive sandwich assay for the detection of Alicyclobacillus acidoterrestris in apple juice based on magnetosome immunomagnetic separation combined with quantum dots immunoassay technology

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The contamination of apple juice by Alicyclobacillus acidoterrestris (A. acidoterrestris) can cause significant economic losses. Therefore, developing a rapid and sensitive method for detecting A. acidoterrestris is necessary. To address this issue, this study prepared magnetosome–monoclonal antibodies immunomagnetic microspheres (IMMs) as capture probes to enrich A. acidoterrestris (IMMs-Aa) and monoclonal antibodies-quantum dots (mAb-CdTeQDs) as detection probes for detecting A. acidoterrestris. The obtained IMMs-Aa-mAb-CdTeQDs “sandwich” structure was used to detect A. acidoterrestris based on the fluorescence intensity. The strategy presented a good linear correlation (y = 1048× + 1891, R² = 0.995) between the fluorescence intensity and the concentration of A. acidoterrestris (10¹–10⁵ CFU/mL) with a low detection limit of 25.4 CFU/mL within 50 min. The recovery rate of this strategy in spiked apple juice ranged from 88.16 % to 107.03 %. Thus, this study established an efficient and highly sensitive magnetic capture-enrichment-separation-detection method for A. acidoterrestris.

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.