耐单宁乳酸菌与单宁酸结合使用对青贮苯乙烯发酵质量、蛋白酶活性和细菌群落的影响。

IF 3.3 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2024-11-20 DOI:10.1002/jsfa.14027

Xiang Guo, Dandan Chen, Peishan Huang, Lin Gao, Wei Zhou, Jianguo Zhang, Qing Zhang

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引用次数: 0

摘要

背景：腐熟过程中的蛋白质分解主要是由于微生物和植物蛋白酶的不良活性造成的，这减少了反刍家畜的蛋白质供应，并造成一系列环境问题。本研究的目的是调查耐单宁酸乳酸菌株 Lactiplantibacillus plantarum 4（LABLP4）与单宁酸（TA）结合使用对苯乙烯类青贮饲料蛋白质保存的影响。青贮苯乙烯类动物时，要么不加添加剂（对照组），要么用 LABLP4（每克鲜物质 106 个菌落形成单位）、1%（鲜物质基）TA、2% TA、LABLP4 + 1% TA 和 LABLP4 + 2% TA 处理。在腌制 3、7、14 和 31 天时测定发酵质量、蛋白质组成、蛋白酶活性和细菌多样性：结果：LABLP4 和 TA 的组合比单独使用 LABLP4 或 TA 降低了 pH 值、大肠菌群数量、非蛋白氮、氨氮（NH3-N）含量和蛋白酶活性（P 3-N 含量）：耐单宁的 LABLP4 和 TA 的组合通过改变细菌群落结构，有效改善了苯乙烯青贮饲料的发酵质量并减少了蛋白质降解。© 2024 化学工业协会。

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Effects of tannin-tolerant lactic acid bacteria in combination with tannic acid on the fermentation quality, protease activity and bacterial community of stylo silage

BACKGROUND

Proteolysis during ensiling primarily occurs due to undesirable microbial and plant protease activities, which reduce the protein supply to ruminant livestock and cause a series of environmental problems. The objective of this study was to investigate the effects of the tannin-tolerant lactic acid bacterium strain Lactiplantibacillus plantarum 4 (LABLP4) in combination with tannic acid (TA) on protein preservation in stylo (Stylosanthes guianensis) silage. The stylos were either ensiled without additives (control) or treated with LABLP4 (10⁶ colony-forming units per gram of fresh matter), 1% (fresh matter basis) TA, 2% TA, LABLP4 + 1% TA and LABLP4 + 2% TA. Fermentation quality, protein composition, protease activity and bacterial diversity were determined at 3, 7, 14 and 31 days of ensiling.

RESULTS

The combination of LABLP4 and TA decreased the pH, coliform bacteria count, non-protein nitrogen, ammonia-nitrogen (NH₃-N) content and protease activities (P < 0.05) and increased the true protein content (P < 0.05) compared to the control. LABLP4 + TA led to a lower pH and NH₃-N content than LABLP4 or TA alone (P < 0.05). On the last day (31 days) of ensiling, LABLP4 + TA increased the relative abundances of Firmicutes and Lactiplantibacillus (P < 0.05), except for the LABLP4 treatment, and decreased the relative abundance of Actinobacteria (P < 0.05).

CONCLUSION

The combination of tannin-tolerant LABLP4 and TA effectively improved the fermentation quality of stylo silage and reduced protein degradation by altering the bacterial community structure. © 2024 Society of Chemical Industry.

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

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