Yuitsu Otsuka, Moriyuki Kawauchi, Kai Yoshizawa, Saori Endo, Kim Schiphof, Kenya Tsuji, Akira Yoshimi, Chihiro Tanaka, Shigekazu Yano, Vladimir Elisashvili, Takehito Nakazawa, Toshikazu Irie, Yoichi Honda
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引用次数: 0
摘要
pkac2 是蛋白激酶 A 的催化亚基基因,它调控与细胞壁合成有关的几个转录因子。Δpkac2菌株的液体培养物显示出极高的菌丝分散性,与野生型菌株(WT)有明显不同。虽然琼脂培养基上的生长速度比 WT 慢,但 Δpkac2 菌株在液体培养物中的生长速度更快,培养 5 d 后的菌丝干重约为 WT 的两倍。显微镜观察显示,与 WT 相比,Δpkac2 菌株的细胞壁更薄。虽然β-葡聚糖合成酶基因的转录水平增加了,但pkac2干扰物细胞壁中的β-葡聚糖含量却降低了。此外,与 WT 相比,Δpkac2 株系的疏水性和抗逆性都有所下降。这些结果表明,破坏 P. ostreatus 的 pkac2 基因会改变细胞壁表层的结构,从而导致菌丝分散造成高密度培养。
Disruption of the pkac2 gene in Pleurotus ostreatus alters cell wall structures and enables mycelial dispersion in liquid culture.
In this study, we developed a mycelial dispersion strain by disrupting the pkac2 gene in the white-rot fungus Pleurotus ostreatus. pkac2 is a catalytic subunit gene of protein kinase A, which regulates several transcription factors related to cell wall synthesis. Liquid cultures of the Δpkac2 strains showed very high mycelial dispersibility and were visibly different from the wild-type (WT) strain. Although growth on agar medium was slower than that of WT, Δpkac2 strains grew faster in liquid culture and had approximately twice the mycelial dry weight of WT after 5 days of culture. Microscopic observations showed that the cell walls of the Δpkac2 strains were thinner compared to WT. The β-glucan content in the cell walls decreased in the pkac2 disruptants, although the transcription levels of β-glucan synthase genes increased. Furthermore, the Δpkac2 strains showed decreased hydrophobicity and stress tolerance compared to WT. These results indicate that disruption of the pkac2 gene in P. ostreatus alters the structure of the cell wall surface layer, resulting in high-density cultures due to mycelial dispersion.
期刊介绍:
FEMS Microbiology Letters gives priority to concise papers that merit rapid publication by virtue of their originality, general interest and contribution to new developments in microbiology. All aspects of microbiology, including virology, are covered.
2019 Impact Factor: 1.987, Journal Citation Reports (Source Clarivate, 2020)
Ranking: 98/135 (Microbiology)
The journal is divided into eight Sections:
Physiology and Biochemistry (including genetics, molecular biology and ‘omic’ studies)
Food Microbiology (from food production and biotechnology to spoilage and food borne pathogens)
Biotechnology and Synthetic Biology
Pathogens and Pathogenicity (including medical, veterinary, plant and insect pathogens – particularly those relating to food security – with the exception of viruses)
Environmental Microbiology (including ecophysiology, ecogenomics and meta-omic studies)
Virology (viruses infecting any organism, including Bacteria and Archaea)
Taxonomy and Systematics (for publication of novel taxa, taxonomic reclassifications and reviews of a taxonomic nature)
Professional Development (including education, training, CPD, research assessment frameworks, research and publication metrics, best-practice, careers and history of microbiology)
If you are unsure which Section is most appropriate for your manuscript, for example in the case of transdisciplinary studies, we recommend that you contact the Editor-In-Chief by email prior to submission. Our scope includes any type of microorganism - all members of the Bacteria and the Archaea and microbial members of the Eukarya (yeasts, filamentous fungi, microbial algae, protozoa, oomycetes, myxomycetes, etc.) as well as all viruses.