浆果发育过程中刺葡萄（Vitis davidii Foëx）组织中原花青素成分、生物合成和组织化学的动态变化。

IF 3.3 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2024-11-20 DOI:10.1002/jsfa.14041

Yinfang Yan, Lin Li, Mingyuan Zheng, Bingbing Duan, Jiajing Zhang, Yashan Li, Wei Liu, Xu Liu

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引用次数: 0

摘要

背景：刺葡萄对湿热气候的适应性很强，因此在中国南方被广泛种植。作为原产于中国的野生物种，刺葡萄（Vitis davidii Foëx）是一种原花青素（PAs）资源。研究分析了浆果发育过程中不同组织（果皮、种子和茎）中原花青素的组成、生物合成和组织化学：结果：研究结果表明，PA 的积累与开花同时发生，并在成熟时完成。高效液相色谱结果表明，表儿茶素是最主要的类型。果皮更容易积累 PA 聚合物。反转录定量聚合酶链反应分析表明，结构基因（黄酮-3'-羟化酶、黄酮-3'5'-羟化酶、二氢黄酮醇 4-还原酶、白花青素还原酶和白花青素二氧酶）的表达水平与 PA 的动态变化呈正相关。组织化学结果显示，果皮中的 PAs 主要存在于外果皮的下真皮层，种子中的 PAs 主要存在于种皮外被膜的中层，茎中的 PAs 主要存在于韧皮部：本研究清楚地了解了刺葡萄中 PAs 的空间和时间积累情况，为分析 PAs 的结构特征、合成及其生物效应奠定了基础。© 2024 化学工业协会。

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Dynamic changes in proanthocyanidin composition, biosynthesis, and histochemistry in spine grape (Vitis davidii Foëx) tissues during berry development.

Background: Spine grapes are widely cultivated in southern China because of their strong adaptability to hot and humid climates. As a wild species native to China, spine grape (Vitis davidii Foëx) was studied as a resource of proanthocyanidins (PAs). PA composition, biosynthesis, and histochemistry in different tissues (skins, seeds, and stems) during berry development were analyzed in this study.

Results: The findings revealed that PA accumulation occurred in concurrence with flowering and was completed by veraison. High-performance liquid chromatographic results showed that the epicatechin type was the most dominant. The skins were more likely to accumulate PA polymers. Reverse transcription-quantitative polymerase chain reaction analysis revealed that the expression levels of structural genes (flavonoid-3'-hydroxylase, flavonoid-3'5'-hydroxylase, dihydroflavonol 4-reductase, leucoanthocyanidin reductase, and leucoanthocyanidin dioxygenase) were positively associated with PA dynamic changes. Histochemical results revealed that PAs in skins were mainly found in the hypodermis of the exocarp, PAs in seeds were mainly found in the middle layer of the outer integument of the testa, and PAs in stems were mainly found in the phloem.

Conclusion: This study provides a clear understanding of the spatial and temporal accumulation of PAs in spine grape, and forms a basis for the analysis of structural profiles and synthesis of PAs and their biological effects. © 2024 Society of Chemical Industry.

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.