补充锌或铜对注射 A 型肉毒毒素 "Botox "在白化大鼠咀嚼肌上的疗效和持续性的影响。

IF 2.2 3区 医学 Q2 Dentistry
Yasmin Saad Elgendy, Sanaa Elzoghby, Nermeen AbuBakr
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引用次数: 0

摘要

研究目的材料与方法:32 只成年雄性白化大鼠平均分为四组:I 组(对照组)、II 组接受 10U BTX-A 注射液、III 组接受 10U BTX-A 注射液 + 锌(1 毫克,注射前 4 天)、IV 组接受 10U BTX-A 注射液 + 铜(0.04 毫克,注射后 7 天)。大鼠在注射后 2 周和 12 周安乐死(每组 4 只)。通过组织学、组织化学、组织形态计量学和实时聚合酶链反应(qRT-PCR)分析对大鼠的颌下肌进行检查:结果:与对照组相比,BTX-A 组的组织病理学结果显示肌肉纤维萎缩,且萎缩程度随时间增加。与 BTX-A 组相比,BTX-A + 锌组的萎缩程度更严重。相反,与 BTX-A 组和 BTX-A + 锌组相比,BTX-A + 铜组的肌肉纤维组织形态有所改善。2 周和 12 周的 Masson 三色染色组织形态分析显示,BTX-A + 铜组和对照组的胶原面积百分比最高,其次是 BTX-A 组和 BTX-A + 锌组。12周时,与BTX-A +铜组相比,BTX-A +锌组和BTX-A组的核因子卡巴β(NF-κB)mRNA表达明显高于对照组:结论:补锌可明显提高 BTX-A 的有效性和持久性,而补铜会降低其有效性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of zinc or copper supplementation on the efficacy and sustainability of botulinum toxin A "Botox" injection in masseter muscle of albino rats.

Objectives: This study aimed to evaluate whether oral zinc or copper supplementation affected the efficacy and sustainability of botulinum toxin-A (BTX-A) injection in masseter muscle of albino rats.

Materials and methods: 32 adult male albino rats were allocated equally into four groups: group I (control), group II received 10U BTX-A injection, group III received 10U BTX-A injection + zinc (1 mg, 4 days pre-injection), and group IV received 10U BTX-A injection + copper (0.04 mg, 7 days post-injection). Rats were euthanized at 2 and 12 weeks (4 rats per subgroup) after injection. The masseter muscle was examined via histological, histochemical, histomorphometrical and real-time polymerase chain reaction (qRT-PCR) analyses.

Results: The histopathological results of the BTX-A group showed atrophied muscle fibers with increased atrophy with time compared to the control group. The BTX-A + zinc group displayed more atrophy compared to BTX-A group. Conversely, the BTX-A + copper group demonstrated improved histology of muscle fibers compared to BTX-A and BTX-A + zinc groups. Histomorphometric analysis of Masson trichrome staining at 2 and 12 weeks revealed that collagen area percentage was the highest in the BTX-A + copper and control groups, followed by BTX-A and BTX-A + zinc groups. At 12 weeks, the nuclear factor kappa beta (NF-κB) mRNA expression was significantly higher in BTX-A + zinc and BTX-A groups compared to BTX-A + copper group and relative to the control group.

Conclusion: Zinc supplementation significantly improved the effectiveness and durability of BTX-A, whereas copper supplementation reduced its efficacy.

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来源期刊
CiteScore
2.20
自引率
9.10%
发文量
305
期刊介绍: J Stomatol Oral Maxillofac Surg publishes research papers and techniques - (guest) editorials, original articles, reviews, technical notes, case reports, images, letters to the editor, guidelines - dedicated to enhancing surgical expertise in all fields relevant to oral and maxillofacial surgery: from plastic and reconstructive surgery of the face, oral surgery and medicine, … to dentofacial and maxillofacial orthopedics. Original articles include clinical or laboratory investigations and clinical or equipment reports. Reviews include narrative reviews, systematic reviews and meta-analyses. All manuscripts submitted to the journal are subjected to peer review by international experts, and must: Be written in excellent English, clear and easy to understand, precise and concise; Bring new, interesting, valid information - and improve clinical care or guide future research; Be solely the work of the author(s) stated; Not have been previously published elsewhere and not be under consideration by another journal; Be in accordance with the journal''s Guide for Authors'' instructions: manuscripts that fail to comply with these rules may be returned to the authors without being reviewed. Under no circumstances does the journal guarantee publication before the editorial board makes its final decision. The journal is indexed in the main international databases and is accessible worldwide through the ScienceDirect and ClinicalKey Platforms.
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