补充锌或铜对注射 A 型肉毒毒素 "Botox "在白化大鼠咀嚼肌上的疗效和持续性的影响。

IF 1.8 3区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Stomatology Oral and Maxillofacial Surgery Pub Date : 2024-11-15 DOI:10.1016/j.jormas.2024.102156

Yasmin Saad Elgendy , Sanaa Elzoghby , Nermeen AbuBakr

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引用次数: 0

摘要

研究目的材料与方法：32 只成年雄性白化大鼠平均分为四组：I 组（对照组）、II 组接受 10U BTX-A 注射液、III 组接受 10U BTX-A 注射液 + 锌（1 毫克，注射前 4 天）、IV 组接受 10U BTX-A 注射液 + 铜（0.04 毫克，注射后 7 天）。大鼠在注射后 2 周和 12 周安乐死（每组 4 只）。通过组织学、组织化学、组织形态计量学和实时聚合酶链反应（qRT-PCR）分析对大鼠的颌下肌进行检查：结果：与对照组相比，BTX-A 组的组织病理学结果显示肌肉纤维萎缩，且萎缩程度随时间增加。与 BTX-A 组相比，BTX-A + 锌组的萎缩程度更严重。相反，与 BTX-A 组和 BTX-A + 锌组相比，BTX-A + 铜组的肌肉纤维组织形态有所改善。2 周和 12 周的 Masson 三色染色组织形态分析显示，BTX-A + 铜组和对照组的胶原面积百分比最高，其次是 BTX-A 组和 BTX-A + 锌组。12周时，与BTX-A +铜组相比，BTX-A +锌组和BTX-A组的核因子卡巴β（NF-κB）mRNA表达明显高于对照组：结论：补锌可明显提高 BTX-A 的有效性和持久性，而补铜会降低其有效性。

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Effect of zinc or copper supplementation on the efficacy and sustainability of botulinum toxin A “Botox” injection in masseter muscle of albino rats

Objectives

This study aimed to evaluate whether oral zinc or copper supplementation affected the efficacy and sustainability of botulinum toxin-A (BTX-A) injection in masseter muscle of albino rats.

Materials and methods

32 adult male albino rats were allocated equally into four groups: group I (control), group II received 10U BTX-A injection, group III received 10U BTX-A injection + zinc (1 mg, 4 days pre-injection), and group IV received 10U BTX-A injection + copper (0.04 mg, 7 days post-injection). Rats were euthanized at 2 and 12 weeks (4 rats per subgroup) after injection. The masseter muscle was examined via histological, histochemical, histomorphometrical and real-time polymerase chain reaction (qRT-PCR) analyses.

Results

The histopathological results of the BTX-A group showed atrophied muscle fibers with increased atrophy with time compared to the control group. The BTX-A + zinc group displayed more atrophy compared to BTX-A group. Conversely, the BTX-A + copper group demonstrated improved histology of muscle fibers compared to BTX-A and BTX-A + zinc groups. Histomorphometric analysis of Masson trichrome staining at 2 and 12 weeks revealed that collagen area percentage was the highest in the BTX-A + copper and control groups, followed by BTX-A and BTX-A + zinc groups. At 12 weeks, the nuclear factor kappa beta (NF-κB) mRNA expression was significantly higher in BTX-A + zinc and BTX-A groups compared to BTX-A + copper group and relative to the control group.