配备定制 ASIC 的频域仪器,用于双斜率近红外光谱分析。

IF 1.3 4区 工程技术 Q3 INSTRUMENTS & INSTRUMENTATION
Alper Kılıç, Giles Blaney, Fatemeh Tavakoli, Jodee Frias, Angelo Sassaroli, Sergio Fantini, Valencia Koomson
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引用次数: 0

摘要

实时和无创测量组织中氧合血红蛋白(HbO2)和脱氧血红蛋白(HbR)的浓度对研究和临床使用都非常有价值。频域近红外光谱仪(FD-NIRS)可以无创测量人体组织中这些发色团的浓度。我们展示了一种用于绝对光学测量的小型、双波长、微型 FD-NIRS 仪器,该仪器由定制的特定应用集成电路和双斜率/自校准(DS/SC)探头组成。调制频率为 55 MHz,采用异调技术进行强度和相位读出,采集速率为 0.7 Hz。仪器由一块 14 × 17 平方厘米的印刷电路板(PCB)、一个 Raspberry Pi 4、一个 STM32G491 微控制器和 DS/SC 探头组成。DS/SC 方法使该仪器能够选择性地测量深层组织,并在无需校准的情况下进行绝对测量。该仪器最初使用组织模拟实体模型进行验证,在确认其适用于体内后,对人体进行了血管闭塞实验。在模型实验中,测量到的平均相位噪声为 0.08°,平均强度标准偏差为 0.10%,光源-探测器距离为 35 毫米。在十帧的窗口中,吸收系数和还原散射系数的平均精确度(测量值随时间的变化)分别为 0.5% 和 0.9%。体内实验的结果表明,在所有测试的测量类型(即 SC、DS 强度和 DS 相位)中,HbO2 和 HbR 浓度都出现了预期的增加。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Frequency-domain instrument with custom ASIC for dual-slope near-infrared spectroscopy.

Real-time and non-invasive measurements of tissue concentrations of oxyhemoglobin (HbO2) and deoxyhemoglobin (HbR) are invaluable for research and clinical use. Frequency-domain near-infrared spectroscopy (FD-NIRS) enables non-invasive measurement of these chromophore concentrations in human tissue. We present a small form factor, dual-wavelength, miniaturized FD-NIRS instrument for absolute optical measurements, built around a custom application-specific integrated circuit and a dual-slope/self-calibrating (DS/SC) probe. The modulation frequency is 55 MHz, and the heterodyning technique was used for intensity and phase readout, with an acquisition rate of 0.7 Hz. The instrument consists of a 14 × 17 cm2 printed circuit board (PCB), a Raspberry Pi 4, an STM32G491 microcontroller, and the DS/SC probe. The DS/SC approach enables this instrument to be selective to deeper tissue and conduct absolute measurements without calibration. The instrument was initially validated using a tissue-mimicking solid phantom, and upon confirming its suitability for in vivo, a vascular occlusion experiment on a human subject was conducted. For the phantom experiments, an average of 0.08° phase noise and 0.10% standard deviation over the mean for the intensities was measured at a source-detector distance of 35 mm. The absorption and reduced scattering coefficients had average precisions (variation of measurement over time) of 0.5% and 0.9%, respectively, on a window of ten frames. Results from the in vivo experiment yielded the expected increase in HbO2 and HbR concentration for all measurement types tested, namely SC, DS intensity, and DS phase.

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来源期刊
Review of Scientific Instruments
Review of Scientific Instruments 工程技术-物理:应用
CiteScore
3.00
自引率
12.50%
发文量
758
审稿时长
2.6 months
期刊介绍: Review of Scientific Instruments, is committed to the publication of advances in scientific instruments, apparatuses, and techniques. RSI seeks to meet the needs of engineers and scientists in physics, chemistry, and the life sciences.
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