螺旋微通道周期性侧向迁移过程中细胞定位的预测模型

IF 6.7 1区 化学 Q1 CHEMISTRY, ANALYTICAL
Kai Zheng, Zhaomiao Liu*, Yan Pang*, Xiang Wang, Siyu Zhao, Nan Zheng, Fanming Cai and Chenchen Zhang, 
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引用次数: 0

摘要

亚微米级细胞的周期性横向迁移是导致细胞分离过程中螺旋微通道精度低的主要因素。本研究针对周期性横向迁移过程中细胞的横向位置推导出了一个数学预测模型(PM)。我们分析了迁移周期、迁移宽度和横向迁移起点与微通道结构和流动条件的关系,并确定了 PM 中的经验系数。结果表明,微通道的长宽比和雷诺数(Re)是影响细胞横向迁移周期性的关键因素。横向迁移宽度受 Re、细胞堵塞率和微通道曲率半径的共同影响。微通道的入口结构和细胞样本与鞘流速的比率是调节初始位置的关键参数。此外,入口处的压力场结构也限制了横向迁移起点的分布范围。无论颗粒/细胞经历 0.5 个、1 个还是多个横向迁移周期,PM 预测的横向位置都与实验观测结果十分吻合,从而验证了 PM 的准确性。这项研究有助于阐明细胞在螺旋微通道中周期性横向迁移的特点,为开发和优化用于精确细胞分离的微型螺旋微通道芯片提供了实用指导。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Predictive Model for Cell Positioning during Periodic Lateral Migration in Spiral Microchannels

Predictive Model for Cell Positioning during Periodic Lateral Migration in Spiral Microchannels

The periodic lateral migration of submicrometer cells is the primary factor leading to low precision in a spiral microchannel during cell isolation. In this study, a mathematical predictive model (PM) is derived for the lateral position of cells during the periodic lateral migration process. We analyze the relationship of migration period, migration width, and starting point of lateral migration with microchannel structure and flow conditions and determine the empirical coefficients in PM. Results indicate that the aspect ratio of the microchannel and the Reynolds number (Re) are key factors that influence the periodicity of the cell lateral migration. The lateral migration width is jointly affected by Re, the cell blockage ratio, and the microchannel curvature radius. The inlet structure of the microchannel and the ratio of the cell sample to the sheath flow rate are critical parameters for regulating the initial position. Moreover, the structure of the pressure field at the inlet constrains the distribution range of the starting point of the lateral migration. Regardless of whether the particles/cells undergo 0.5, 1, or multiple lateral migration cycles, the lateral positions predicted by PM align well with the experimental observations, thus verifying the accuracy of PM. This research helps to elucidate the characteristics of periodic lateral migration of cells in spiral microchannels and can provide practical guidance for the development and optimization of miniature spiral microchannel chips for precise cell isolation.

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来源期刊
Analytical Chemistry
Analytical Chemistry 化学-分析化学
CiteScore
12.10
自引率
12.20%
发文量
1949
审稿时长
1.4 months
期刊介绍: Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.
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