{"title":"优化水产蛋白:各种预处理方法对提高小黑豆副产品再循环潜力的启示","authors":"JingChao Yu, Weon-Sun Shin","doi":"10.1016/j.foodchem.2024.142029","DOIUrl":null,"url":null,"abstract":"<div><div>To minimize resource waste and promote recycling, it is essential to recover nutritional components from by-products. This study aimed to optimize of Aquasoya protein (AS-P) recovery from small black bean by-products, focusing on the effects of pH adjustments, sonication, and fractionation on protein yield, solubility, and structural integrity. Alkaline treatment at pH 8.5 significantly enhanced AS-P recovery yield (77.60 %) and protein concentration (421.16 μg/mL), attributed to the increased solubility and effective removal of non-protein components. Sonication for 10 min exhibited the highest protein solubility (88.69 %) post-fractionation, indicating reduced protein aggregation. Fractionation was crucial in minimizing browning reactions and enhancing protein purity by eliminating oligosaccharides and impurities. SDS-PAGE analysis revealed distinct protein bands from alkaline treatment, while sonication resulted in lipoxygenase (LOX) protein degradation. Principal Component Analysis (PCA) emphasized that fractionation notably enhances the stability and quality of AS-P, making it as a viable ingredient for food applications.</div></div>","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"465 ","pages":"Article 142029"},"PeriodicalIF":8.5000,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Optimizing aquasoya protein: Insights of various pre-treatments for enhancing the upcycling potential from small black bean by-product\",\"authors\":\"JingChao Yu, Weon-Sun Shin\",\"doi\":\"10.1016/j.foodchem.2024.142029\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>To minimize resource waste and promote recycling, it is essential to recover nutritional components from by-products. This study aimed to optimize of Aquasoya protein (AS-P) recovery from small black bean by-products, focusing on the effects of pH adjustments, sonication, and fractionation on protein yield, solubility, and structural integrity. Alkaline treatment at pH 8.5 significantly enhanced AS-P recovery yield (77.60 %) and protein concentration (421.16 μg/mL), attributed to the increased solubility and effective removal of non-protein components. Sonication for 10 min exhibited the highest protein solubility (88.69 %) post-fractionation, indicating reduced protein aggregation. Fractionation was crucial in minimizing browning reactions and enhancing protein purity by eliminating oligosaccharides and impurities. SDS-PAGE analysis revealed distinct protein bands from alkaline treatment, while sonication resulted in lipoxygenase (LOX) protein degradation. Principal Component Analysis (PCA) emphasized that fractionation notably enhances the stability and quality of AS-P, making it as a viable ingredient for food applications.</div></div>\",\"PeriodicalId\":318,\"journal\":{\"name\":\"Food Chemistry\",\"volume\":\"465 \",\"pages\":\"Article 142029\"},\"PeriodicalIF\":8.5000,\"publicationDate\":\"2024-11-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Food Chemistry\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0308814624036793\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, APPLIED\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Chemistry","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0308814624036793","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
Optimizing aquasoya protein: Insights of various pre-treatments for enhancing the upcycling potential from small black bean by-product
To minimize resource waste and promote recycling, it is essential to recover nutritional components from by-products. This study aimed to optimize of Aquasoya protein (AS-P) recovery from small black bean by-products, focusing on the effects of pH adjustments, sonication, and fractionation on protein yield, solubility, and structural integrity. Alkaline treatment at pH 8.5 significantly enhanced AS-P recovery yield (77.60 %) and protein concentration (421.16 μg/mL), attributed to the increased solubility and effective removal of non-protein components. Sonication for 10 min exhibited the highest protein solubility (88.69 %) post-fractionation, indicating reduced protein aggregation. Fractionation was crucial in minimizing browning reactions and enhancing protein purity by eliminating oligosaccharides and impurities. SDS-PAGE analysis revealed distinct protein bands from alkaline treatment, while sonication resulted in lipoxygenase (LOX) protein degradation. Principal Component Analysis (PCA) emphasized that fractionation notably enhances the stability and quality of AS-P, making it as a viable ingredient for food applications.
期刊介绍:
Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.