在工程大肠杆菌中生产不含抗生素的高级蛋氨酸

IF 5.7 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY
Lijuan Wang, Yingying Guo, Mengyue Li, Xiaowen Chen, Kun Yang, Zhiqiang Liu, Yuguo Zheng
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引用次数: 0

摘要

l-蛋氨酸是一种宝贵的含硫氨基酸,在饲料添加剂、营养保健品、药品甚至化妆品行业中都具有重要意义。然而,由于蛋氨酸的生物合成途径复杂以及发酵过程中质粒的丢失,实现蛋氨酸的高效微生物生产仍具有挑战性。在本文中,通过加强琥珀酰-CoA的供应、引入直接硫化途径和削弱l-苏氨酸支链途径,改进了l-蛋氨酸的生物合成。在 5 升生物反应器中,工程菌株产生了 21.55 克/升的蛋氨酸,葡萄糖产量为 0.14 克/克。为了消除对抗生素的需求并尽量减少质粒的损失,质粒中加入了 hok/sok 系统。由此产生的质粒 pAm10 使菌株 M2 thrBA1G 在 5 升饲料批量培养中无需抗生素即可生产出 20.39 克/升的蛋氨酸,比对照组提高了 42.58%。这项研究凸显了基于质粒的无抗生素发酵法在高效、低成本生产蛋氨酸以及其他氨基酸或化学品方面的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Antibiotic-Free High-Level l-Methionine Production in Engineered Escherichia coli

Antibiotic-Free High-Level l-Methionine Production in Engineered Escherichia coli
l-Methionine, a valuable sulfur-containing amino acid, holds great significance as a feed additive, nutraceutical, pharmaceutical, or even in the cosmetic industry. However, achieving efficient microbial production of l-methionine remains challenging due to its complex biosynthetic pathway and plasmid loss during fermentation. Herein, l-methionine biosynthesis was improved by enhancing succinyl-CoA supply, introducing a direct-sulfurylation pathway, and weakening the l-threonine branched pathway. The engineered strain produced 21.55 g/L l-methionine with a yield of 0.14 g/g glucose in a 5 L bioreactor. To eliminate the need for antibiotics and minimize plasmid loss, the hok/sok system was incorporated into the plasmid. The resulting plasmid pAm10 enabled strain M2 thrBA1G to produce 20.39 g/L of l-methionine without antibiotics in 5 L of fed-batch cultivation, a 42.58% increase compared to the control. This study highlights the potential of plasmid-based antibiotic-free fermentation for efficient and cost-effective production of l-methionine, as well as other amino acids or chemicals.
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来源期刊
Journal of Agricultural and Food Chemistry
Journal of Agricultural and Food Chemistry 农林科学-农业综合
CiteScore
9.90
自引率
8.20%
发文量
1375
审稿时长
2.3 months
期刊介绍: The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.
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