Mengqi Chen , Miaoxi Peng , Muyun Yuan , Chengdong Huang , Jingwen Liu , Zuqing Wu , Wenrui Chen , Songqing Hu , Qing Liu , Jie Dong , Li Ling
{"title":"利用靶向质谱法检测食品中的肠炎沙门氏菌","authors":"Mengqi Chen , Miaoxi Peng , Muyun Yuan , Chengdong Huang , Jingwen Liu , Zuqing Wu , Wenrui Chen , Songqing Hu , Qing Liu , Jie Dong , Li Ling","doi":"10.1016/j.foodchem.2024.141985","DOIUrl":null,"url":null,"abstract":"<div><div>The high prevalence of <em>Salmonella enterica</em> necessitates rapid and efficient detection methods. Targeted mass spectrometry (MS) using multiple reaction monitoring (MRM) and parallel reaction monitoring (PRM) has become a promising technique with improved specificity and sensitivity. We develop a novel targeted MS method for detecting <em>S. enterica</em> in food based on peptide biomarkers. Using a combination of four peptide biomarkers, this newly developed method could accurately distinguish <em>S. enterica</em> from other conventional food-borne pathogens. When combined with buoyant density centrifugation (BDC), <em>Salmonella</em> was efficiently separated from food matrices. Based on this discovery, this method was successfully applied to detect <em>S. enterica</em> in both artificially and naturally contaminated food samples, comparable to the culture method. These results demonstrate the potential of the targeted MS method in various food categories and are expected to be an alternative approach for <em>S. enterica</em> detection in food.</div></div>","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"465 ","pages":"Article 141985"},"PeriodicalIF":8.5000,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Detection of Salmonella enterica in food using targeted mass spectrometry\",\"authors\":\"Mengqi Chen , Miaoxi Peng , Muyun Yuan , Chengdong Huang , Jingwen Liu , Zuqing Wu , Wenrui Chen , Songqing Hu , Qing Liu , Jie Dong , Li Ling\",\"doi\":\"10.1016/j.foodchem.2024.141985\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The high prevalence of <em>Salmonella enterica</em> necessitates rapid and efficient detection methods. Targeted mass spectrometry (MS) using multiple reaction monitoring (MRM) and parallel reaction monitoring (PRM) has become a promising technique with improved specificity and sensitivity. We develop a novel targeted MS method for detecting <em>S. enterica</em> in food based on peptide biomarkers. Using a combination of four peptide biomarkers, this newly developed method could accurately distinguish <em>S. enterica</em> from other conventional food-borne pathogens. When combined with buoyant density centrifugation (BDC), <em>Salmonella</em> was efficiently separated from food matrices. Based on this discovery, this method was successfully applied to detect <em>S. enterica</em> in both artificially and naturally contaminated food samples, comparable to the culture method. These results demonstrate the potential of the targeted MS method in various food categories and are expected to be an alternative approach for <em>S. enterica</em> detection in food.</div></div>\",\"PeriodicalId\":318,\"journal\":{\"name\":\"Food Chemistry\",\"volume\":\"465 \",\"pages\":\"Article 141985\"},\"PeriodicalIF\":8.5000,\"publicationDate\":\"2024-11-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Food Chemistry\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0308814624036355\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, APPLIED\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Chemistry","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0308814624036355","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
Detection of Salmonella enterica in food using targeted mass spectrometry
The high prevalence of Salmonella enterica necessitates rapid and efficient detection methods. Targeted mass spectrometry (MS) using multiple reaction monitoring (MRM) and parallel reaction monitoring (PRM) has become a promising technique with improved specificity and sensitivity. We develop a novel targeted MS method for detecting S. enterica in food based on peptide biomarkers. Using a combination of four peptide biomarkers, this newly developed method could accurately distinguish S. enterica from other conventional food-borne pathogens. When combined with buoyant density centrifugation (BDC), Salmonella was efficiently separated from food matrices. Based on this discovery, this method was successfully applied to detect S. enterica in both artificially and naturally contaminated food samples, comparable to the culture method. These results demonstrate the potential of the targeted MS method in various food categories and are expected to be an alternative approach for S. enterica detection in food.
期刊介绍:
Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.