鉴定骨干网干细胞因子及其定位机制

IF 44.7 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Science Pub Date : 2024-11-07 DOI:10.1126/science.adj8752

Gugan Eswaran, Xixi Zhang, Jacob Pieter Rutten, Jingyi Han, Hiroyuki Iida, Jennifer López Ortiz, Riikka Mäkilä, Brecht Wybouw, Benjamin Planterose Jiménez, Leo Vainio, Alexis Porcher, Marina Leal Gavarron, Jing Zhang, Tiina Blomster, Xin Wang, David Dolan, Ondřej Smetana, Siobhán M. Brady, Melis Kucukoglu Topcu, Kirsten ten Tusscher, J. Peter Etchells, Ari Pekka Mähönen

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引用次数: 0

摘要

木材是陆地生物量的最大储存库。木材由木质部组成，来自维管凸起的一侧，这是一个双面干细胞龛，在对立面也产生韧皮部。目前还不知道哪些分子因子赋予了韧皮部干细胞的特性。在这里，我们展示了TRACHEARY ELEMENT DIFFERENTIATION INHIBITORY FACTOR (TDIF)配体激活的PHLOEM INTERCALATED WITH XYLEM (PXY)受体可促进CAMBIUM-EXPRESSED AINTEGUMENTA-LIKE(CAIL)转录因子的表达，从而确定拟南芥根部的韧皮部干细胞特征。木质部表达的 PXY 通过封存韧皮部起源的 TDIF，限制了 TDIF 信号转导前沿，导致 CAIL 的表达被激活，干细胞特征只在一个狭窄的区域内表达。我们的研究结果表明，来自对侧细胞的信号如何确保双面干细胞层的稳健、动态可调定位。

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Identification of cambium stem cell factors and their positioning mechanism

Wood constitutes the largest reservoir of terrestrial biomass. Composed of xylem, it arises from one side of the vascular cambium, a bifacial stem cell niche that also produces phloem on the opposing side. It is currently unknown which molecular factors endow cambium stem cell identity. Here we show that TRACHEARY ELEMENT DIFFERENTIATION INHIBITORY FACTOR (TDIF) ligand–activated PHLOEM INTERCALATED WITH XYLEM (PXY) receptors promote the expression of CAMBIUM-EXPRESSED AINTEGUMENTA-LIKE (CAIL) transcription factors to define cambium stem cell identity in the Arabidopsis root. By sequestrating the phloem-originated TDIF, xylem-expressed PXY confines the TDIF signaling front, resulting in the activation of CAIL expression and stem cell identity in only a narrow domain. Our findings show how signals emanating from cells on opposing sides ensure robust yet dynamically adjustable positioning of a bifacial stem cell layer.

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来源期刊

Science 综合性期刊-综合性期刊

CiteScore

61.10

自引率

0.90%

发文量

审稿时长

2.1 months

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