北京鸭长链脂肪酸（C≥16）伸长和脱饱和关键基因的转录活性和变异分析

IF 3.8 1区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Poultry Science Pub Date : 2024-10-18 DOI:10.1016/j.psj.2024.104437

Dandan Sun , Yongtong Liu , Xiaoqin Li, Mengqi Ge, Meiyi Zhu, Yuqin He, Zhuocheng Hou

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引用次数: 0

摘要

为了加深我们对鸭体内长链脂肪酸碳链伸长和脱饱和的了解，本研究系统分析了关键基因启动子的转录活性，包括 ELOVLs、FADSs 和 SCDs。预测建模和统计分析揭示了转录因子结合基序的普遍性，特别是那些与 Sp1、NF-1 和 C/EBPalpha 相关的基序。此外，通过对野鸭和家鸭（特别是野鸭和北京鸭）的重测序数据进行变异分析，发现了 ELOVL2、ELOVL5 和 ELOVL6 核心启动子区域的定向诱变。值得注意的是，ELOVL2 中 -56 G>C 位置、ELOVL5 中 -52 T>C 位置和 ELOVL6 中 -513 T>C 位置的突变显著降低了转录活性。这些发现大大加深了我们对鸭子长链脂肪酸生物合成的分子调控机制的理解，并支持了未来旨在培育营养价值更高的北京鸭品种的遗传选育计划。

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Transcriptional activity and variation analysis of genes critical for long-chain fatty acid (C≥16) elongation and desaturation in Pekin ducks

To deepen our understanding of long-chain fatty acid carbon chain elongation and desaturation in ducks, this study systematically analyzed the transcriptional activities of key gene promoters, including ELOVLs, FADSs, and SCDs. Predictive modeling coupled with statistical analysis revealed a prevalence of binding motifs for transcription factors, particularly those associated with Sp1, NF-1, and C/EBPalpha. Moreover, variation analysis of resequencing data from both wild and domestic ducks, specifically mallards and Pekin ducks, informed targeted mutagenesis within the core promoter regions of ELOVL2, ELOVL5, and ELOVL6. Notably, mutations at positions -56 G>C in ELOVL2, -52 T>C in ELOVL5, and -513 T>C in ELOVL6 significantly diminished transcriptional activity. These findings substantially enhance our understanding of the molecular mechanisms regulating the biosynthesis of long-chain fatty acids in ducks and support future genetic selection initiatives aimed at developing Pekin duck breeds with enhanced nutritional value.

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来源期刊

Poultry Science 农林科学-奶制品与动物科学

CiteScore

7.60

自引率

15.90%

发文量

审稿时长

94 days

期刊介绍： First self-published in 1921, Poultry Science is an internationally renowned monthly journal, known as the authoritative source for a broad range of poultry information and high-caliber research. The journal plays a pivotal role in the dissemination of preeminent poultry-related knowledge across all disciplines. As of January 2020, Poultry Science will become an Open Access journal with no subscription charges, meaning authors who publish here can make their research immediately, permanently, and freely accessible worldwide while retaining copyright to their work. Papers submitted for publication after October 1, 2019 will be published as Open Access papers. An international journal, Poultry Science publishes original papers, research notes, symposium papers, and reviews of basic science as applied to poultry. This authoritative source of poultry information is consistently ranked by ISI Impact Factor as one of the top 10 agriculture, dairy and animal science journals to deliver high-caliber research. Currently it is the highest-ranked (by Impact Factor and Eigenfactor) journal dedicated to publishing poultry research. Subject areas include breeding, genetics, education, production, management, environment, health, behavior, welfare, immunology, molecular biology, metabolism, nutrition, physiology, reproduction, processing, and products.