通过水线给药的双菌株益生菌能在肉鸡面临菌群失调挑战时,有效调节回肠和盲肠微生物群、sIgA 和急性期蛋白水平以及肉鸡的生长性能。

IF 3.8 1区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
S A S van der Klein, S S Arora, S Haldar, A K Dhara, K Gibbs
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引用次数: 0

摘要

由于饲料中抗生素用量的减少,肉鸡肠道菌痢的发病率正在上升。本研究测试了在三步微生物挑战过程中,每天在水线上添加由嗜酸乳杆菌 AG01 和动物双歧杆菌亚种乳杆菌 AG02 组成的双菌株益生菌对生长性能和肠道健康的影响。在完全随机设计中,总共有 900 只罗斯 308 雄鸡被分配到 36 个地板围栏(25 只/围栏,12 个围栏/处理)。饲喂以玉米、小麦和豆粕为基础的饲料。日粮分 3 个阶段配制(开食期:1 至 10 日龄;生长期:11 至 24 日龄;育成期:25 至 42 日龄)。处理包括无挑战对照组(NC)、有挑战对照组(CC)和添加 1 × 108 菌落总数(CFU)/只/天益生菌的对照组(CC+益生菌)。挑战包括第 7 天 1 × 108 CFU/只禽的禽致病性大肠杆菌、第 15 天 4,000 个卵囊/只禽的艾美耳菌以及第 18、19 和 20 天 1 × 109 CFU/只禽的产气荚膜杆菌。在 42 天内监测生长性能,收集血液样本和消化液,并进行肠道菌群失调评分。与 NC 禽类相比,CC 禽类表现出体重下降(所有阶段)、采食量减少(开食期和生长期)、FCR 增加(生长期和总体;P < 0.05)、回肠乳酸菌浓度降低(第 24 天和第 42 天)和盲肠大肠杆菌增加(第 24 天;P < 0.05)。与 CC 鸡相比,CC+益生菌鸡在生长期的体重、增重和采食量均有所增加(P < 0.05),第 24 天和第 42 天回肠乳酸菌增加,第 24 天回肠产气荚膜杆菌减少,第 42 天粘膜分泌型 IgA 增加,血清甲型 1-酸糖蛋白减少。CC+益生菌禽类的总体生长性能与NC禽类相当。这些结果证实了双菌株益生菌在减轻多种微生物挑战的负面影响、改善肠道健康和生长性能方面的功效。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A dual strain probiotic administered via the waterline beneficially modulates the ileal and cecal microbiome, sIgA and acute phase protein levels, and growth performance of broilers during a dysbacteriosis challenge.

Intestinal dysbacteriosis is increasing in broilers due to the reduced use of antibiotics in feed. This study tested the effect of daily waterline administration of a dual-strain probiotic comprising Lactobacillus acidophilus AG01 and Bifidobacterium animalis subspecies lactis AG02, on growth performance and intestinal health during a 3-step microbial challenge. In total, 900 Ross 308 males were assigned to 36 floor pens (25 birds/pen, 12 pens/treatment) in a completely randomized design. Birds were fed a corn, wheat and soybean-meal based diet. Diets were formulated in 3 phases (starter: 1 to 10; grower: 11 to 24; finisher: 25 to 42 d of age). Treatments comprised a non-challenged control (NC), challenged control (CC), and the CC supplemented with 1 × 108 colony forming units (CFU)/bird/day of the probiotic (CC+Probiotic). The challenge comprised 1 × 108 CFU/bird of Avian Pathogenic Escherichia coli on d 7, 4,000 oocysts/bird of Eimeria on d 15 and 1 × 109 CFU/bird of C. perfringens on d 18, 19 and 20. Growth performance was monitored over 42 d, blood samples, and digesta were collected and intestinal dysbacteriosis scoring was performed. Compared to NC birds, CC birds exhibited reduced BW (all phases), reduced feed intake (starter and grower phase), increased FCR (grower phase and overall; P < 0.05), reduced ileal lactic acid bacteria concentrations (d 24 and 42), and increased cecal E. coli (d 24; P < 0.05). Compared to CC birds, CC+Probiotic birds exhibited increased BW, BW gain and feed intake during grower phase (P < 0.05), increased ileal lactic acid bacteria at d 24 and 42 and reduced ileal C. perfringens at d 24, increased mucosal secretory IgA and reduced serum alpha-1-acid-glycoprotein at d 42. The overall growth performance of CC+Probiotic birds was equivalent to NC birds. These results confirm the efficacy of the dual strain probiotic for mitigating the negative effects of a multi-microbial challenge, improving gut health and growth performance in commercial broilers under dysbacteriosis challenge.

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来源期刊
Poultry Science
Poultry Science 农林科学-奶制品与动物科学
CiteScore
7.60
自引率
15.90%
发文量
0
审稿时长
94 days
期刊介绍: First self-published in 1921, Poultry Science is an internationally renowned monthly journal, known as the authoritative source for a broad range of poultry information and high-caliber research. The journal plays a pivotal role in the dissemination of preeminent poultry-related knowledge across all disciplines. As of January 2020, Poultry Science will become an Open Access journal with no subscription charges, meaning authors who publish here can make their research immediately, permanently, and freely accessible worldwide while retaining copyright to their work. Papers submitted for publication after October 1, 2019 will be published as Open Access papers. An international journal, Poultry Science publishes original papers, research notes, symposium papers, and reviews of basic science as applied to poultry. This authoritative source of poultry information is consistently ranked by ISI Impact Factor as one of the top 10 agriculture, dairy and animal science journals to deliver high-caliber research. Currently it is the highest-ranked (by Impact Factor and Eigenfactor) journal dedicated to publishing poultry research. Subject areas include breeding, genetics, education, production, management, environment, health, behavior, welfare, immunology, molecular biology, metabolism, nutrition, physiology, reproduction, processing, and products.
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