S A S van der Klein, S S Arora, S Haldar, A K Dhara, K Gibbs
{"title":"通过水线给药的双菌株益生菌能在肉鸡面临菌群失调挑战时,有效调节回肠和盲肠微生物群、sIgA 和急性期蛋白水平以及肉鸡的生长性能。","authors":"S A S van der Klein, S S Arora, S Haldar, A K Dhara, K Gibbs","doi":"10.1016/j.psj.2024.104462","DOIUrl":null,"url":null,"abstract":"<p><p>Intestinal dysbacteriosis is increasing in broilers due to the reduced use of antibiotics in feed. This study tested the effect of daily waterline administration of a dual-strain probiotic comprising Lactobacillus acidophilus AG01 and Bifidobacterium animalis subspecies lactis AG02, on growth performance and intestinal health during a 3-step microbial challenge. In total, 900 Ross 308 males were assigned to 36 floor pens (25 birds/pen, 12 pens/treatment) in a completely randomized design. Birds were fed a corn, wheat and soybean-meal based diet. Diets were formulated in 3 phases (starter: 1 to 10; grower: 11 to 24; finisher: 25 to 42 d of age). Treatments comprised a non-challenged control (NC), challenged control (CC), and the CC supplemented with 1 × 10<sup>8</sup> colony forming units (CFU)/bird/day of the probiotic (CC+Probiotic). The challenge comprised 1 × 10<sup>8</sup> CFU/bird of Avian Pathogenic Escherichia coli on d 7, 4,000 oocysts/bird of Eimeria on d 15 and 1 × 10<sup>9</sup> CFU/bird of C. perfringens on d 18, 19 and 20. Growth performance was monitored over 42 d, blood samples, and digesta were collected and intestinal dysbacteriosis scoring was performed. Compared to NC birds, CC birds exhibited reduced BW (all phases), reduced feed intake (starter and grower phase), increased FCR (grower phase and overall; P < 0.05), reduced ileal lactic acid bacteria concentrations (d 24 and 42), and increased cecal E. coli (d 24; P < 0.05). Compared to CC birds, CC+Probiotic birds exhibited increased BW, BW gain and feed intake during grower phase (P < 0.05), increased ileal lactic acid bacteria at d 24 and 42 and reduced ileal C. perfringens at d 24, increased mucosal secretory IgA and reduced serum alpha-1-acid-glycoprotein at d 42. The overall growth performance of CC+Probiotic birds was equivalent to NC birds. These results confirm the efficacy of the dual strain probiotic for mitigating the negative effects of a multi-microbial challenge, improving gut health and growth performance in commercial broilers under dysbacteriosis challenge.</p>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":null,"pages":null},"PeriodicalIF":3.8000,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A dual strain probiotic administered via the waterline beneficially modulates the ileal and cecal microbiome, sIgA and acute phase protein levels, and growth performance of broilers during a dysbacteriosis challenge.\",\"authors\":\"S A S van der Klein, S S Arora, S Haldar, A K Dhara, K Gibbs\",\"doi\":\"10.1016/j.psj.2024.104462\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Intestinal dysbacteriosis is increasing in broilers due to the reduced use of antibiotics in feed. This study tested the effect of daily waterline administration of a dual-strain probiotic comprising Lactobacillus acidophilus AG01 and Bifidobacterium animalis subspecies lactis AG02, on growth performance and intestinal health during a 3-step microbial challenge. In total, 900 Ross 308 males were assigned to 36 floor pens (25 birds/pen, 12 pens/treatment) in a completely randomized design. Birds were fed a corn, wheat and soybean-meal based diet. Diets were formulated in 3 phases (starter: 1 to 10; grower: 11 to 24; finisher: 25 to 42 d of age). Treatments comprised a non-challenged control (NC), challenged control (CC), and the CC supplemented with 1 × 10<sup>8</sup> colony forming units (CFU)/bird/day of the probiotic (CC+Probiotic). The challenge comprised 1 × 10<sup>8</sup> CFU/bird of Avian Pathogenic Escherichia coli on d 7, 4,000 oocysts/bird of Eimeria on d 15 and 1 × 10<sup>9</sup> CFU/bird of C. perfringens on d 18, 19 and 20. Growth performance was monitored over 42 d, blood samples, and digesta were collected and intestinal dysbacteriosis scoring was performed. Compared to NC birds, CC birds exhibited reduced BW (all phases), reduced feed intake (starter and grower phase), increased FCR (grower phase and overall; P < 0.05), reduced ileal lactic acid bacteria concentrations (d 24 and 42), and increased cecal E. coli (d 24; P < 0.05). Compared to CC birds, CC+Probiotic birds exhibited increased BW, BW gain and feed intake during grower phase (P < 0.05), increased ileal lactic acid bacteria at d 24 and 42 and reduced ileal C. perfringens at d 24, increased mucosal secretory IgA and reduced serum alpha-1-acid-glycoprotein at d 42. The overall growth performance of CC+Probiotic birds was equivalent to NC birds. These results confirm the efficacy of the dual strain probiotic for mitigating the negative effects of a multi-microbial challenge, improving gut health and growth performance in commercial broilers under dysbacteriosis challenge.</p>\",\"PeriodicalId\":20459,\"journal\":{\"name\":\"Poultry Science\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.8000,\"publicationDate\":\"2024-10-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Poultry Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1016/j.psj.2024.104462\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Poultry Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1016/j.psj.2024.104462","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
A dual strain probiotic administered via the waterline beneficially modulates the ileal and cecal microbiome, sIgA and acute phase protein levels, and growth performance of broilers during a dysbacteriosis challenge.
Intestinal dysbacteriosis is increasing in broilers due to the reduced use of antibiotics in feed. This study tested the effect of daily waterline administration of a dual-strain probiotic comprising Lactobacillus acidophilus AG01 and Bifidobacterium animalis subspecies lactis AG02, on growth performance and intestinal health during a 3-step microbial challenge. In total, 900 Ross 308 males were assigned to 36 floor pens (25 birds/pen, 12 pens/treatment) in a completely randomized design. Birds were fed a corn, wheat and soybean-meal based diet. Diets were formulated in 3 phases (starter: 1 to 10; grower: 11 to 24; finisher: 25 to 42 d of age). Treatments comprised a non-challenged control (NC), challenged control (CC), and the CC supplemented with 1 × 108 colony forming units (CFU)/bird/day of the probiotic (CC+Probiotic). The challenge comprised 1 × 108 CFU/bird of Avian Pathogenic Escherichia coli on d 7, 4,000 oocysts/bird of Eimeria on d 15 and 1 × 109 CFU/bird of C. perfringens on d 18, 19 and 20. Growth performance was monitored over 42 d, blood samples, and digesta were collected and intestinal dysbacteriosis scoring was performed. Compared to NC birds, CC birds exhibited reduced BW (all phases), reduced feed intake (starter and grower phase), increased FCR (grower phase and overall; P < 0.05), reduced ileal lactic acid bacteria concentrations (d 24 and 42), and increased cecal E. coli (d 24; P < 0.05). Compared to CC birds, CC+Probiotic birds exhibited increased BW, BW gain and feed intake during grower phase (P < 0.05), increased ileal lactic acid bacteria at d 24 and 42 and reduced ileal C. perfringens at d 24, increased mucosal secretory IgA and reduced serum alpha-1-acid-glycoprotein at d 42. The overall growth performance of CC+Probiotic birds was equivalent to NC birds. These results confirm the efficacy of the dual strain probiotic for mitigating the negative effects of a multi-microbial challenge, improving gut health and growth performance in commercial broilers under dysbacteriosis challenge.
期刊介绍:
First self-published in 1921, Poultry Science is an internationally renowned monthly journal, known as the authoritative source for a broad range of poultry information and high-caliber research. The journal plays a pivotal role in the dissemination of preeminent poultry-related knowledge across all disciplines. As of January 2020, Poultry Science will become an Open Access journal with no subscription charges, meaning authors who publish here can make their research immediately, permanently, and freely accessible worldwide while retaining copyright to their work. Papers submitted for publication after October 1, 2019 will be published as Open Access papers.
An international journal, Poultry Science publishes original papers, research notes, symposium papers, and reviews of basic science as applied to poultry. This authoritative source of poultry information is consistently ranked by ISI Impact Factor as one of the top 10 agriculture, dairy and animal science journals to deliver high-caliber research. Currently it is the highest-ranked (by Impact Factor and Eigenfactor) journal dedicated to publishing poultry research. Subject areas include breeding, genetics, education, production, management, environment, health, behavior, welfare, immunology, molecular biology, metabolism, nutrition, physiology, reproduction, processing, and products.