Inhibitory effect of tea flower polysaccharides on oxidative stress and microglial oxidative damage in aging mice by regulating gut microbiota.

Tea flower polysaccharides (TFPS) have prominent anti-aging effect. In this study, we used an animal model of aging induced by D-galactose in mice to investigate the effect of TFPS on reducing inflammatory factors, lowering oxidative stress levels, and inhibiting oxidative damage to microglia from the perspective of regulating gut microbiota. The results showed that TFPS could improve the homeostasis of gut microbiota in aging mice, reduce the ratio of Firmicutes to Bacteroidota, and significantly increase the abundance of Lactobacillus. At the same time, TFPS reduced the excessive activation of hippocampal microglia in aging mice, significantly down-regulated the levels of pro-inflammatory factors IL-6, IL-1β, TNF-α, and nuclear transcription factor NF-κB, increased the activity of antioxidant enzymes SOD, CAT, and POD, and reduced the content of MDA. Our research results indicate that TFPS can improve the disorder of gut microbiota, alleviate oxidative damage to glial cells, alleviate neuroinflammation, and play a role in delaying aging.