{"title":"为分析水牛肉中的零容忍抗菌素开发一种有针对性的 LC-MS/MS 方法","authors":"","doi":"10.1016/j.jfca.2024.106858","DOIUrl":null,"url":null,"abstract":"<div><div>A simple, affordable, and efficient multiresidue analytical method was developed and validated using liquid chromatography-tandem mass spectrometry (LC-MS/MS) to accurately identify and quantify a prioritized zero-tolerance antimicrobial residues in buffalo meat. Analyzing zero-tolerance antimicrobials such as chloramphenicol, metronidazole, and nitrofurans involves detecting even trace levels of residues in food products to safeguard consumer safety and public health. A modified QuEChERS method with 2-nitrobenzaldehyde derivatization using ethyl acetate was employed for the extraction of the antimicrobial compounds, followed by a suitable clean-up. Liquid chromatographic conditions were optimized on a reverse phase C18 analytical column over a runtime of 12 min, and the eluted analytes were observed in selected reaction monitoring mode (SRM). The method's performance was validated in accordance with Regulation (EU) 2019/1871 and Commission Implementing Regulation (EU) 2021/810 guidelines. The linearity of the calibration curves was > 0.99, and the mean recoveries of the analytes ranged from 81.92 % to 110 %. The intra-day and inter-day precisions were less than 9 %. The limits of detection (LOD) and limits of quantification (LOQ) were in the range of 0.04–0.11 µg/kg and 0.13–0.37 µg/kg, respectively. This is well below the MRLs set by the European Commission for zero-tolerance substances.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":null,"pages":null},"PeriodicalIF":4.0000,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Development of a targeted LC-MS/MS method for the analysis of zero tolerance antimicrobials in buffalo meat\",\"authors\":\"\",\"doi\":\"10.1016/j.jfca.2024.106858\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>A simple, affordable, and efficient multiresidue analytical method was developed and validated using liquid chromatography-tandem mass spectrometry (LC-MS/MS) to accurately identify and quantify a prioritized zero-tolerance antimicrobial residues in buffalo meat. Analyzing zero-tolerance antimicrobials such as chloramphenicol, metronidazole, and nitrofurans involves detecting even trace levels of residues in food products to safeguard consumer safety and public health. A modified QuEChERS method with 2-nitrobenzaldehyde derivatization using ethyl acetate was employed for the extraction of the antimicrobial compounds, followed by a suitable clean-up. Liquid chromatographic conditions were optimized on a reverse phase C18 analytical column over a runtime of 12 min, and the eluted analytes were observed in selected reaction monitoring mode (SRM). The method's performance was validated in accordance with Regulation (EU) 2019/1871 and Commission Implementing Regulation (EU) 2021/810 guidelines. The linearity of the calibration curves was > 0.99, and the mean recoveries of the analytes ranged from 81.92 % to 110 %. The intra-day and inter-day precisions were less than 9 %. The limits of detection (LOD) and limits of quantification (LOQ) were in the range of 0.04–0.11 µg/kg and 0.13–0.37 µg/kg, respectively. This is well below the MRLs set by the European Commission for zero-tolerance substances.</div></div>\",\"PeriodicalId\":15867,\"journal\":{\"name\":\"Journal of Food Composition and Analysis\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.0000,\"publicationDate\":\"2024-10-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Food Composition and Analysis\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0889157524008925\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, APPLIED\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Food Composition and Analysis","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0889157524008925","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
Development of a targeted LC-MS/MS method for the analysis of zero tolerance antimicrobials in buffalo meat
A simple, affordable, and efficient multiresidue analytical method was developed and validated using liquid chromatography-tandem mass spectrometry (LC-MS/MS) to accurately identify and quantify a prioritized zero-tolerance antimicrobial residues in buffalo meat. Analyzing zero-tolerance antimicrobials such as chloramphenicol, metronidazole, and nitrofurans involves detecting even trace levels of residues in food products to safeguard consumer safety and public health. A modified QuEChERS method with 2-nitrobenzaldehyde derivatization using ethyl acetate was employed for the extraction of the antimicrobial compounds, followed by a suitable clean-up. Liquid chromatographic conditions were optimized on a reverse phase C18 analytical column over a runtime of 12 min, and the eluted analytes were observed in selected reaction monitoring mode (SRM). The method's performance was validated in accordance with Regulation (EU) 2019/1871 and Commission Implementing Regulation (EU) 2021/810 guidelines. The linearity of the calibration curves was > 0.99, and the mean recoveries of the analytes ranged from 81.92 % to 110 %. The intra-day and inter-day precisions were less than 9 %. The limits of detection (LOD) and limits of quantification (LOQ) were in the range of 0.04–0.11 µg/kg and 0.13–0.37 µg/kg, respectively. This is well below the MRLs set by the European Commission for zero-tolerance substances.
期刊介绍:
The Journal of Food Composition and Analysis publishes manuscripts on scientific aspects of data on the chemical composition of human foods, with particular emphasis on actual data on composition of foods; analytical methods; studies on the manipulation, storage, distribution and use of food composition data; and studies on the statistics, use and distribution of such data and data systems. The Journal''s basis is nutrient composition, with increasing emphasis on bioactive non-nutrient and anti-nutrient components. Papers must provide sufficient description of the food samples, analytical methods, quality control procedures and statistical treatments of the data to permit the end users of the food composition data to evaluate the appropriateness of such data in their projects.
The Journal does not publish papers on: microbiological compounds; sensory quality; aromatics/volatiles in food and wine; essential oils; organoleptic characteristics of food; physical properties; or clinical papers and pharmacology-related papers.