Maisa Kasanga, Márió Gajdács, Walter Muleya, Odion O Ikhimiukor, Steward Mudenda, Maika Kasanga, Joseph Chizimu, Doreen Mainza Shempela, Benjamin Bisesa Solochi, Mark John Mwikisa, Kaunda Yamba, Cheryl P Andam, Raphael Chanda, Duncan Chanda, Geoffrey Kwenda
{"title":"赞比亚卢萨卡人、动物和环境中产扩展谱 β-乳酰胺酶大肠埃希氏菌的基因型特征和抗菌药耐药性：公共卫生影响和统一健康监测。","authors":"Maisa Kasanga, Márió Gajdács, Walter Muleya, Odion O Ikhimiukor, Steward Mudenda, Maika Kasanga, Joseph Chizimu, Doreen Mainza Shempela, Benjamin Bisesa Solochi, Mark John Mwikisa, Kaunda Yamba, Cheryl P Andam, Raphael Chanda, Duncan Chanda, Geoffrey Kwenda","doi":"10.3390/antibiotics13100951","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Extended-spectrum β-lactamases (ESBL) in <i>Escherichia coli</i> are a serious concern due to their role in developing multidrug resistance (MDR) and difficult-to-treat infections.</p><p><strong>Objective: </strong>This study aimed to identify ESBL-carrying <i>E. coli</i> strains from both clinical and environmental sources in Lusaka District, Zambia.</p><p><strong>Methods: </strong>This cross-sectional study included 58 ESBL-producing <i>E. coli</i> strains from hospital inpatients, outpatients, and non-hospital environments. Antimicrobial susceptibility was assessed using the Kirby-Bauer disk diffusion method and the VITEK<sup>®</sup> 2 Compact System, while genotypic analyses utilised the Illumina NextSeq 2000 sequencing platform.</p><p><strong>Results: </strong>Among the strains isolated strains, phylogroup B2 was the most common, with resistant MLST sequence types including ST131, ST167, ST156, and ST69. ESBL genes such as <i>bla</i><sub>TEM-1B</sub>, <i>bla</i><sub>CTX-M,</sub><i>bla</i><sub>OXA-1</sub>, <i>bla</i><sub>NDM-5</sub>, and <i>bla</i><sub>CMY</sub> were identified, with ST131 and ST410 being the most common. ST131 exhibited a high prevalence of <i>bla</i><sub>CTX-M-15</sub> and resistance to fluoroquinolones. Clinical and environmental isolates carried <i>bla</i><sub>NDM-5</sub> (3.4%), with clinical isolates showing a higher risk of carbapenemase resistance genes and the frequent occurrence of <i>bla</i><sub>CTX-M</sub> and <i>bla</i><sub>TEM</sub> variants, especially <i>bla</i><sub>CTX-M-15</sub> in ST131.</p><p><strong>Conclusions: </strong>This study underscores the public health risks of <i>bla</i><sub>CTX-M-15</sub>- and <i>bla</i><sub>NDM-5</sub>-carrying <i>E. coli</i>. The strengthening antimicrobial stewardship programmes and the continuous surveillance of AMR in clinical and environmental settings are recommended to mitigate the spread of resistant pathogens.</p>","PeriodicalId":54246,"journal":{"name":"Antibiotics-Basel","volume":"13 10","pages":""},"PeriodicalIF":4.3000,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11505244/pdf/","citationCount":"0","resultStr":"{\"title\":\"Genotypic Characterisation and Antimicrobial Resistance of Extended-Spectrum β-Lactamase-Producing <i>Escherichia coli</i> in Humans, Animals, and the Environment from Lusaka, Zambia: Public Health Implications and One Health Surveillance.\",\"authors\":\"Maisa Kasanga, Márió Gajdács, Walter Muleya, Odion O Ikhimiukor, Steward Mudenda, Maika Kasanga, Joseph Chizimu, Doreen Mainza Shempela, Benjamin Bisesa Solochi, Mark John Mwikisa, Kaunda Yamba, Cheryl P Andam, Raphael Chanda, Duncan Chanda, Geoffrey Kwenda\",\"doi\":\"10.3390/antibiotics13100951\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Extended-spectrum β-lactamases (ESBL) in <i>Escherichia coli</i> are a serious concern due to their role in developing multidrug resistance (MDR) and difficult-to-treat infections.</p><p><strong>Objective: </strong>This study aimed to identify ESBL-carrying <i>E. coli</i> strains from both clinical and environmental sources in Lusaka District, Zambia.</p><p><strong>Methods: </strong>This cross-sectional study included 58 ESBL-producing <i>E. coli</i> strains from hospital inpatients, outpatients, and non-hospital environments. Antimicrobial susceptibility was assessed using the Kirby-Bauer disk diffusion method and the VITEK<sup>®</sup> 2 Compact System, while genotypic analyses utilised the Illumina NextSeq 2000 sequencing platform.</p><p><strong>Results: </strong>Among the strains isolated strains, phylogroup B2 was the most common, with resistant MLST sequence types including ST131, ST167, ST156, and ST69. ESBL genes such as <i>bla</i><sub>TEM-1B</sub>, <i>bla</i><sub>CTX-M,</sub><i>bla</i><sub>OXA-1</sub>, <i>bla</i><sub>NDM-5</sub>, and <i>bla</i><sub>CMY</sub> were identified, with ST131 and ST410 being the most common. ST131 exhibited a high prevalence of <i>bla</i><sub>CTX-M-15</sub> and resistance to fluoroquinolones. Clinical and environmental isolates carried <i>bla</i><sub>NDM-5</sub> (3.4%), with clinical isolates showing a higher risk of carbapenemase resistance genes and the frequent occurrence of <i>bla</i><sub>CTX-M</sub> and <i>bla</i><sub>TEM</sub> variants, especially <i>bla</i><sub>CTX-M-15</sub> in ST131.</p><p><strong>Conclusions: </strong>This study underscores the public health risks of <i>bla</i><sub>CTX-M-15</sub>- and <i>bla</i><sub>NDM-5</sub>-carrying <i>E. coli</i>. The strengthening antimicrobial stewardship programmes and the continuous surveillance of AMR in clinical and environmental settings are recommended to mitigate the spread of resistant pathogens.</p>\",\"PeriodicalId\":54246,\"journal\":{\"name\":\"Antibiotics-Basel\",\"volume\":\"13 10\",\"pages\":\"\"},\"PeriodicalIF\":4.3000,\"publicationDate\":\"2024-10-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11505244/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Antibiotics-Basel\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.3390/antibiotics13100951\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Antibiotics-Basel","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3390/antibiotics13100951","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
Genotypic Characterisation and Antimicrobial Resistance of Extended-Spectrum β-Lactamase-Producing Escherichia coli in Humans, Animals, and the Environment from Lusaka, Zambia: Public Health Implications and One Health Surveillance.
Background: Extended-spectrum β-lactamases (ESBL) in Escherichia coli are a serious concern due to their role in developing multidrug resistance (MDR) and difficult-to-treat infections.
Objective: This study aimed to identify ESBL-carrying E. coli strains from both clinical and environmental sources in Lusaka District, Zambia.
Methods: This cross-sectional study included 58 ESBL-producing E. coli strains from hospital inpatients, outpatients, and non-hospital environments. Antimicrobial susceptibility was assessed using the Kirby-Bauer disk diffusion method and the VITEK® 2 Compact System, while genotypic analyses utilised the Illumina NextSeq 2000 sequencing platform.
Results: Among the strains isolated strains, phylogroup B2 was the most common, with resistant MLST sequence types including ST131, ST167, ST156, and ST69. ESBL genes such as blaTEM-1B, blaCTX-M,blaOXA-1, blaNDM-5, and blaCMY were identified, with ST131 and ST410 being the most common. ST131 exhibited a high prevalence of blaCTX-M-15 and resistance to fluoroquinolones. Clinical and environmental isolates carried blaNDM-5 (3.4%), with clinical isolates showing a higher risk of carbapenemase resistance genes and the frequent occurrence of blaCTX-M and blaTEM variants, especially blaCTX-M-15 in ST131.
Conclusions: This study underscores the public health risks of blaCTX-M-15- and blaNDM-5-carrying E. coli. The strengthening antimicrobial stewardship programmes and the continuous surveillance of AMR in clinical and environmental settings are recommended to mitigate the spread of resistant pathogens.
Antibiotics-BaselPharmacology, Toxicology and Pharmaceutics-General Pharmacology, Toxicology and Pharmaceutics
CiteScore
7.30
自引率
14.60%
发文量
1547
审稿时长
11 weeks
期刊介绍：
Antibiotics (ISSN 2079-6382) is an open access, peer reviewed journal on all aspects of antibiotics. Antibiotics is a multi-disciplinary journal encompassing the general fields of biochemistry, chemistry, genetics, microbiology and pharmacology. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on the length of papers.
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