基于 TMT 的 IBDV 感染 CEF 细胞定量蛋白质组分析揭示了鸡 IRF10 通过抑制 I 型干扰素表达在 IBDV 复制中的有利作用。

IF 3.8 1区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Poultry Science Pub Date : 2024-10-13 DOI:10.1016/j.psj.2024.104421

Shujun Zhang , Jiaxin Li , Hui Gao , Yongqiang Wang , Hong Cao , Xiaoqi Li , Li Gao , Shijun J. Zheng

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引用次数: 0

摘要

传染性法氏囊病（IBD）是由传染性法氏囊病病毒（IBDV）引起的一种急性、高度接触性传染病，给全球家禽业造成了巨大的经济损失。目前，新出现的 IBDV 变异株和新的重组株在许多国家流行，对家禽业的发展构成了严重威胁。阐明 IBDV 感染的发病机理将对开发疫苗控制 IBDV 感染大有帮助。本研究采用液相色谱串联质谱（LC-MS/MS）结合串联质谱标记（TMT）技术测定了感染 IBDV 24 h 后鸡胚成纤维细胞（CEF）细胞核蛋白的表达。我们的数据显示，在 IBDV 感染细胞与模拟感染对照细胞中，共有 236 种细胞核蛋白表达存在差异，其中 171 种蛋白显著上调，65 种蛋白下调。生物信息学分析表明，差异表达蛋白（DEPs）主要参与免疫反应、DNA复制、错配修复和RIG-I样受体（RLR）信号转导。一致的是，经实时定量 PCR（qRT-PCR）检测，10 个选定的上调基因（IRF10、IRF7、IRF1、STAT1、ATF3、GTF3A、CSRP3、RARB、BASP1 和 NF-κB1）的表达明显增加。此外，经 Western Blot 检测，IRF10 在 DF-1 细胞的细胞质和细胞核中的表达均上调。此外，IRF10的敲除通过促进IFN-I反应显著抑制了IBDV的复制，而IRF10的过表达则显著抑制了模拟细胞和IBDV感染细胞中I型干扰素和ISGs的表达，这表明IRF10是宿主抗病毒反应的负调控因子。这些结果为进一步研究宿主与 IBDV 的相互作用以及 IBDV 感染的潜在机制提供了线索。

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TMT-based quantitative proteomic analysis of IBDV-infected CEF cells reveals a favorable role of chicken IRF10 in IBDV replication via suppressing type-I interferon expression

Infectious bursal disease (IBD) is an acute, highly contagious disease caused by infectious bursal disease virus (IBDV), causing huge economic losses to the poultry industry worldwide. Currently, the emerging variant strains of IBDV and new recombinants in the field are circulating in many countries and poses severe threats to the development of poultry industry. Elucidation of the pathogenesis of IBDV infection will be of great help to the development of vaccines for control of IBDV infection. In this study, liquid chromatography tandem-mass spectrometry (LC–MS/MS) combined with tandem mass tag (TMT) labeling was performed to determine the expressions of nucleus proteins in IBDV-infected chicken embryonic fibroblast (CEF) cells 24 h post-infection (hpi). Our data show that a total of 236 nucleus proteins were differentially expressed in IBDV-infected cells vs mock-infected controls, and that among those proteins, 171 were significantly upregulated while 65 downregulated. Bioinformatics analysis reveals that the differentially expressed proteins (DEPs) were mainly involved in immune response, DNA replication, mismatch repair, and RIG-I-like receptor (RLR) signaling. Consistently, the expression of ten selected upregulated genes (IRF10, IRF7, IRF1, STAT1, ATF3, GTF3A, CSRP3, RARB, BASP1, and NF-κB1) markedly increased as examined by quantitative real‐time PCR (qRT-PCR). Furthermore, the expression of IRF10 was upregulated both in the cytoplasm and nucleus of DF-1 cells as examined by Western Blot. Moreover, knockdown of IRF10 remarkably inhibited IBDV replication via promoting IFN-I response, and overexpression of IRF10 significantly suppressed type I interferon and ISGs expression in both mock and IBDV-infected cells, suggesting that IRF10 serve as a negative regulator for host antiviral response. These results provide clues to further investigation into host–IBDV interactions and the underlying mechanisms of IBDV infection.

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来源期刊

Poultry Science 农林科学-奶制品与动物科学

CiteScore

7.60

自引率

15.90%

发文量

审稿时长

94 days

期刊介绍： First self-published in 1921, Poultry Science is an internationally renowned monthly journal, known as the authoritative source for a broad range of poultry information and high-caliber research. The journal plays a pivotal role in the dissemination of preeminent poultry-related knowledge across all disciplines. As of January 2020, Poultry Science will become an Open Access journal with no subscription charges, meaning authors who publish here can make their research immediately, permanently, and freely accessible worldwide while retaining copyright to their work. Papers submitted for publication after October 1, 2019 will be published as Open Access papers. An international journal, Poultry Science publishes original papers, research notes, symposium papers, and reviews of basic science as applied to poultry. This authoritative source of poultry information is consistently ranked by ISI Impact Factor as one of the top 10 agriculture, dairy and animal science journals to deliver high-caliber research. Currently it is the highest-ranked (by Impact Factor and Eigenfactor) journal dedicated to publishing poultry research. Subject areas include breeding, genetics, education, production, management, environment, health, behavior, welfare, immunology, molecular biology, metabolism, nutrition, physiology, reproduction, processing, and products.