多层纳米乳液对β-胡萝卜素体外消化和抗氧化活性的影响

IF 6 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Mei Zi Sun, Do-Yeong Kim, Youjin Baek, Hyeon Gyu Lee
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引用次数: 0

摘要

本研究的目的是采用逐层技术设计多层水包油型纳米乳液,以提高β-胡萝卜素的稳定性,并评估其对体外释放和抗氧化活性的影响。为了制备β-胡萝卜素负载型多层纳米乳液(NEs),在一级NE(PRI-NE)中使用了吐温20,在二级NE(SEC-CS)中使用了壳聚糖(CS),在两种三级NE(TER-DS、TER-SA)中使用了硫酸葡聚糖(DS)和海藻酸钠(SA)。多层 NE 的粒径范围在 92 到 110 nm 之间,具有很高的包埋效率(92%-99%)。在模拟胃肠道模型中培养后,游离脂肪酸的释放率在包覆 CS、DS 和 SA 后略有下降。PRI-NE、SEC-CS、TER-DS 和 TER-SA 的 β 胡萝卜素生物可及性分别为 7.02%、7.96%、10.88% 和 10.25%。与 PRI-NE 相比,多层 NE 的 2,2-二苯基-1-苦基肼自由基清除能力提高了 1.2 倍。此外,TER-DS 的细胞抗氧化能力(87.24%)比 PRI-NE 的细胞抗氧化能力(48.36%)提高了 1.8 倍。因此,多层纳米乳液是提高β-胡萝卜素的稳定性、体外消化和抗氧化活性的一种有潜在价值的技术。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Effect of Multilayer Nanoemulsion on the In Vitro Digestion and Antioxidant Activity of β-Carotene.

The objectives of this study were to design multilayer oil-in-water nanoemulsions using a layer-by-layer technique to enhance the stability of β-carotene and evaluate its effect on in vitro release and antioxidant activity. To prepare β-carotene-loaded multilayer nanoemulsions (NEs), a primary NE (PRI-NE) using Tween 20 was coated with chitosan (CS) for the secondary NE (SEC-CS), and with dextran sulfate (DS) and sodium alginate (SA) for the two types of tertiary NEs (TER-DS, TER-SA). The multilayer NEs ranged in particle size from 92 to 110 nm and exhibited high entrapment efficiency (92-99%). After incubation in a simulated gastrointestinal tract model, the release rate of free fatty acids decreased slightly after coating with CS, DS, and SA. The bioaccessibility of β-carotene was 7.02% for the PRI-NE, 7.96% for the SEC-CS, 10.88% for the TER-DS, and 10.25% for the TER-SA. The 2,2-diphenyl-1-picrylhydrazyl radical scavenging abilities increased by 1.2 times for the multilayer NEs compared to the PRI-NE. In addition, the cellular antioxidant abilities improved by 1.8 times for the TER-DS (87.24%) compared to the PRI-NE (48.36%). Therefore, multilayer nanoemulsions are potentially valuable techniques to improve the stability, in vitro digestion, and antioxidant activity of β-carotene.

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来源期刊
Antioxidants
Antioxidants Biochemistry, Genetics and Molecular Biology-Physiology
CiteScore
10.60
自引率
11.40%
发文量
2123
审稿时长
16.3 days
期刊介绍: Antioxidants (ISSN 2076-3921), provides an advanced forum for studies related to the science and technology of antioxidants. It publishes research papers, reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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