{"title":"南非草药茶的化学分析、酶抑制活性和抗氧化能力:Buddleja saligna、Lippia javanica、L. scaberrima 和 Phyla dulcis。","authors":"Nélida Nina, Alberto Burgos-Edwards, Cristina Theoduloz, Satyajit Tripathy, Motlalepula Gilbert Matsabisa, Guillermo Schmeda-Hirschmann","doi":"10.3390/antiox13101219","DOIUrl":null,"url":null,"abstract":"<p><p>Herbal teas are used in South Africa as digestives to lower glycaemia and for other indications. However, the chemical composition of the infusions and their effect on enzymes related to metabolic syndrome is poorly known. The composition of infusions and methanol extracts of <i>B. saligna</i> (Scrophulariaceae), <i>Lippia javanica</i>, <i>L. scaberrima</i>, and <i>Phyla dulcis</i> (Verbenaceae) was assessed and the effect of the infusions and extract was determined towards α-glucosidase, α-amylase, and pancreatic lipase. The commercial herbal products were extracted separately with MeOH or hot water to obtain the extract/infusion for comparative studies. Total phenolic, total flavonoid and antioxidant capacity were assessed. The fingerprints of the MeOH extracts and infusions were compared by HPLD-DAD. The extract constituents were tentatively identified by HPLC-MS/MS and NMR analyses. From the extracts/infusions, 57 compounds were identified, including iridoids, phenylpropanoid glycosides, flavonoids, and caffeic acid derivatives, among others. The MeOH extracts and infusions showed strong inhibition towards α-glucosidase with IC<sub>50</sub> in the range of 0.13-0.84 µg/mL for the phenolic-enriched infusion extract (PEI) and 0.47-0.50 µg/mL for the MeOH extracts, respectively. The <i>P. dulcis</i> PEI showed higher inhibition towards α-glucosidase, and the MeOH extract of <i>L. scaberrima</i> was better than the PEI. At 100 µg/mL, the PEI from the four herbal teas reduces the activity of α-amylase by 23.03-28.61%, with <i>L. javanica</i> as the most active tea. Three of the species are high in phenylpropanoid glycosides, while <i>P. dulcis</i> contains rosmarinic acid. Some 26 compounds were identified in the infusion from <i>B. saligna</i>, 28 from <i>L. scaberrima</i>, and 21 from <i>P. dulcis</i>. Four of them are common in all the teas, namely decaffeoylverbascoside, verbascoside, isoverbascoside, and tuberonic acid hexoside. Ten compounds occur in two of the teas and seventeen, fifteen, and eleven compounds were detected only in <i>B. saligna</i>, <i>L. scaberrima</i>, and <i>P. dulcis</i>, respectively. Most of the compounds are reported for the first time from the crude drug infusions. The results give some support for the traditional use of herbal teas as digestives and/or indications for diabetes. The chemical fingerprints set the basis for quality control of the crude drugs, based on the main constituents and differential compounds occurring in the samples.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":null,"pages":null},"PeriodicalIF":6.0000,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11505134/pdf/","citationCount":"0","resultStr":"{\"title\":\"Chemical Profiling, Enzyme Inhibitory Activity and Antioxidant Capacity of South African Herbal Teas: <i>Buddleja saligna</i>, <i>Lippia javanica</i>, <i>L. scaberrima</i> and <i>Phyla dulcis</i>.\",\"authors\":\"Nélida Nina, Alberto Burgos-Edwards, Cristina Theoduloz, Satyajit Tripathy, Motlalepula Gilbert Matsabisa, Guillermo Schmeda-Hirschmann\",\"doi\":\"10.3390/antiox13101219\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Herbal teas are used in South Africa as digestives to lower glycaemia and for other indications. 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The MeOH extracts and infusions showed strong inhibition towards α-glucosidase with IC<sub>50</sub> in the range of 0.13-0.84 µg/mL for the phenolic-enriched infusion extract (PEI) and 0.47-0.50 µg/mL for the MeOH extracts, respectively. The <i>P. dulcis</i> PEI showed higher inhibition towards α-glucosidase, and the MeOH extract of <i>L. scaberrima</i> was better than the PEI. At 100 µg/mL, the PEI from the four herbal teas reduces the activity of α-amylase by 23.03-28.61%, with <i>L. javanica</i> as the most active tea. Three of the species are high in phenylpropanoid glycosides, while <i>P. dulcis</i> contains rosmarinic acid. Some 26 compounds were identified in the infusion from <i>B. saligna</i>, 28 from <i>L. scaberrima</i>, and 21 from <i>P. dulcis</i>. Four of them are common in all the teas, namely decaffeoylverbascoside, verbascoside, isoverbascoside, and tuberonic acid hexoside. 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引用次数: 0
摘要
在南非,草药茶被用作消化剂,以降低血糖并用于其他用途。然而,人们对草药茶的化学成分及其对代谢综合征相关酶的影响知之甚少。我们评估了 B. saligna(景天科)、Lippia javanica、L. scaberrima 和 Phyla dulcis(马鞭草科)的输液和甲醇提取物的成分,并确定了输液和提取物对 α-葡萄糖苷酶、α-淀粉酶和胰脂肪酶的影响。商用草药产品分别用 MeOH 或热水提取,得到提取物/浸泡液,用于比较研究。对总酚、总黄酮和抗氧化能力进行了评估。通过 HPLD-DAD 比较了 MeOH 提取物和输液的指纹图谱。通过 HPLC-MS/MS 和 NMR 分析,对提取物成分进行了初步鉴定。从提取物/浸液中鉴定出 57 种化合物,包括虹彩类、苯丙苷类、黄酮类和咖啡酸衍生物等。MeOH提取物和输液对α-葡萄糖苷酶有很强的抑制作用,酚类富集输液提取物(PEI)的IC50范围为0.13-0.84 µg/mL,MeOH提取物的IC50范围为0.47-0.50 µg/mL。P. dulcis PEI 对α-葡萄糖苷酶的抑制率较高,而 L. scaberrima 的 MeOH 提取物的抑制率优于 PEI。在 100 µg/mL 的浓度下,四种凉茶中的 PEI 可使 α 淀粉酶的活性降低 23.03%-28.61%,其中 L. javanica 的活性最高。其中三个品种含有大量苯丙甙,而枳实含有迷迭香酸。在 B. saligna、L. scaberrima 和 P. dulcis 的浸泡液中分别发现了 26 种、28 种和 21 种化合物。其中四种化合物在所有茶叶中都很常见,分别是去咖啡因长链苷,马鞭草苷,异长链苷和块茎酸六甙。有 10 种化合物出现在两种茶叶中,17、15 和 11 种化合物分别只在 B. saligna、L. scaberrima 和 P. dulcis 中检测到。大多数化合物都是首次从粗制药物中检测到的。这些结果为传统上将花草茶用作消化剂和/或糖尿病适应症提供了一些支持。根据样本中出现的主要成分和差异化合物,化学指纹为粗制药物的质量控制奠定了基础。
Chemical Profiling, Enzyme Inhibitory Activity and Antioxidant Capacity of South African Herbal Teas: Buddleja saligna, Lippia javanica, L. scaberrima and Phyla dulcis.
Herbal teas are used in South Africa as digestives to lower glycaemia and for other indications. However, the chemical composition of the infusions and their effect on enzymes related to metabolic syndrome is poorly known. The composition of infusions and methanol extracts of B. saligna (Scrophulariaceae), Lippia javanica, L. scaberrima, and Phyla dulcis (Verbenaceae) was assessed and the effect of the infusions and extract was determined towards α-glucosidase, α-amylase, and pancreatic lipase. The commercial herbal products were extracted separately with MeOH or hot water to obtain the extract/infusion for comparative studies. Total phenolic, total flavonoid and antioxidant capacity were assessed. The fingerprints of the MeOH extracts and infusions were compared by HPLD-DAD. The extract constituents were tentatively identified by HPLC-MS/MS and NMR analyses. From the extracts/infusions, 57 compounds were identified, including iridoids, phenylpropanoid glycosides, flavonoids, and caffeic acid derivatives, among others. The MeOH extracts and infusions showed strong inhibition towards α-glucosidase with IC50 in the range of 0.13-0.84 µg/mL for the phenolic-enriched infusion extract (PEI) and 0.47-0.50 µg/mL for the MeOH extracts, respectively. The P. dulcis PEI showed higher inhibition towards α-glucosidase, and the MeOH extract of L. scaberrima was better than the PEI. At 100 µg/mL, the PEI from the four herbal teas reduces the activity of α-amylase by 23.03-28.61%, with L. javanica as the most active tea. Three of the species are high in phenylpropanoid glycosides, while P. dulcis contains rosmarinic acid. Some 26 compounds were identified in the infusion from B. saligna, 28 from L. scaberrima, and 21 from P. dulcis. Four of them are common in all the teas, namely decaffeoylverbascoside, verbascoside, isoverbascoside, and tuberonic acid hexoside. Ten compounds occur in two of the teas and seventeen, fifteen, and eleven compounds were detected only in B. saligna, L. scaberrima, and P. dulcis, respectively. Most of the compounds are reported for the first time from the crude drug infusions. The results give some support for the traditional use of herbal teas as digestives and/or indications for diabetes. The chemical fingerprints set the basis for quality control of the crude drugs, based on the main constituents and differential compounds occurring in the samples.
AntioxidantsBiochemistry, Genetics and Molecular Biology-Physiology
CiteScore
10.60
自引率
11.40%
发文量
2123
审稿时长
16.3 days
期刊介绍:
Antioxidants (ISSN 2076-3921), provides an advanced forum for studies related to the science and technology of antioxidants. It publishes research papers, reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.