{"title":"Aronia melanocarpa 提取物通过丝裂原活化蛋白激酶 1 延长秀丽隐杆线虫的寿命和健康寿命。","authors":"Huan Zhang, Zhigang Zhu, Wenjing Wei, Zekun Liu, Huiji Zhou, Yueling Gong, Xinlei Yan, Jun Du, Houkai Li, Liang Chen, Lili Sheng","doi":"10.1039/d4fo02479f","DOIUrl":null,"url":null,"abstract":"<p><p>Aging is a highly complex process and one of the largest risk factors for many chronic diseases. <i>Aronia melanocarpa</i> (AM) is rich in bioactive phytochemicals with antioxidant, anti-inflammatory, and anticancer properties. However, little is known about its effects on aging. The objective of this study was to evaluate the effects of AM extract on lifespan and health-span using <i>Caenorhabditis elegans</i> as a representative model. The mechanisms of its effects were explored using transcriptomics and untargeted metabolomics. Results showed that the lifespan of <i>C. elegans</i> was significantly extended by 22.2% after high-dose AM treatment. AM improved the behavior and physiological functions of <i>C. elegans</i> by increasing the pharyngeal pumping rate, decreasing lipofuscin accumulation and the reactive oxygen species level, enhancing resistance to oxidative stress, and increasing the activities of superoxide dismutase and catalase. Transcriptome analysis showed that the <i>pmk-1</i> gene (mitogen-activated protein kinase 1), which is involved in the MAPK signaling pathway, was the gene with the largest fold change after AM intervention. However, in the <i>C. elegans pmk-1(km25)</i> mutant, the beneficial effect of AM in improving nematode senescence disappeared. An untargeted metabolomics study showed that the levels of 4-hydroxyproline, rhamnose, and cysteine were increased after AM supplementation, and their extending effect on the lifespan and health-span of <i>C. elegans</i> were partly dependent on the <i>pmk-1</i> gene. In conclusion, our results revealed that AM can promote the lifespan and health-span of <i>C. elegans via</i> the PMK-1 pathway, highlighting the potential of AM as a dietary supplement to delay aging.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":null,"pages":null},"PeriodicalIF":5.1000,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"<i>Aronia melanocarpa</i> extract extends the lifespan and health-span of <i>Caenorhabditis elegans via</i> mitogen-activated protein kinase 1.\",\"authors\":\"Huan Zhang, Zhigang Zhu, Wenjing Wei, Zekun Liu, Huiji Zhou, Yueling Gong, Xinlei Yan, Jun Du, Houkai Li, Liang Chen, Lili Sheng\",\"doi\":\"10.1039/d4fo02479f\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Aging is a highly complex process and one of the largest risk factors for many chronic diseases. <i>Aronia melanocarpa</i> (AM) is rich in bioactive phytochemicals with antioxidant, anti-inflammatory, and anticancer properties. However, little is known about its effects on aging. The objective of this study was to evaluate the effects of AM extract on lifespan and health-span using <i>Caenorhabditis elegans</i> as a representative model. The mechanisms of its effects were explored using transcriptomics and untargeted metabolomics. Results showed that the lifespan of <i>C. elegans</i> was significantly extended by 22.2% after high-dose AM treatment. AM improved the behavior and physiological functions of <i>C. elegans</i> by increasing the pharyngeal pumping rate, decreasing lipofuscin accumulation and the reactive oxygen species level, enhancing resistance to oxidative stress, and increasing the activities of superoxide dismutase and catalase. Transcriptome analysis showed that the <i>pmk-1</i> gene (mitogen-activated protein kinase 1), which is involved in the MAPK signaling pathway, was the gene with the largest fold change after AM intervention. However, in the <i>C. elegans pmk-1(km25)</i> mutant, the beneficial effect of AM in improving nematode senescence disappeared. An untargeted metabolomics study showed that the levels of 4-hydroxyproline, rhamnose, and cysteine were increased after AM supplementation, and their extending effect on the lifespan and health-span of <i>C. elegans</i> were partly dependent on the <i>pmk-1</i> gene. In conclusion, our results revealed that AM can promote the lifespan and health-span of <i>C. elegans via</i> the PMK-1 pathway, highlighting the potential of AM as a dietary supplement to delay aging.</p>\",\"PeriodicalId\":77,\"journal\":{\"name\":\"Food & Function\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":5.1000,\"publicationDate\":\"2024-10-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Food & Function\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1039/d4fo02479f\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food & Function","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1039/d4fo02479f","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
衰老是一个非常复杂的过程,也是许多慢性疾病的最大风险因素之一。Aronia melanocarpa(AM)富含生物活性植物化学物质,具有抗氧化、抗炎和抗癌特性。然而,人们对它对衰老的影响知之甚少。本研究的目的是以 elegans(秀丽隐杆线虫)为代表模型,评估 AM 提取物对寿命和健康寿命的影响。研究使用转录组学和非靶向代谢组学探讨了其作用机制。结果表明,高剂量 AM 处理后,秀丽隐杆线虫的寿命显著延长了 22.2%。AM通过提高咽泵率、减少脂褐质积累和活性氧水平、增强对氧化应激的抵抗力以及提高超氧化物歧化酶和过氧化氢酶的活性,改善了秀丽隐杆线虫的行为和生理功能。转录组分析表明,参与 MAPK 信号通路的 pmk-1 基因(丝裂原活化蛋白激酶 1)是 AM 干预后折叠变化最大的基因。然而,在秀丽隐杆线虫 pmk-1(km25)突变体中,AM 对改善线虫衰老的有益作用消失了。一项非靶向代谢组学研究表明,补充AM后,4-羟基脯氨酸、鼠李糖和半胱氨酸的含量增加,它们对延长秀丽隐杆线虫寿命和健康寿命的作用部分依赖于pmk-1基因。总之,我们的研究结果表明,AM可通过PMK-1途径促进秀丽隐杆线虫的寿命和健康寿命,凸显了AM作为膳食补充剂延缓衰老的潜力。
Aronia melanocarpa extract extends the lifespan and health-span of Caenorhabditis elegans via mitogen-activated protein kinase 1.
Aging is a highly complex process and one of the largest risk factors for many chronic diseases. Aronia melanocarpa (AM) is rich in bioactive phytochemicals with antioxidant, anti-inflammatory, and anticancer properties. However, little is known about its effects on aging. The objective of this study was to evaluate the effects of AM extract on lifespan and health-span using Caenorhabditis elegans as a representative model. The mechanisms of its effects were explored using transcriptomics and untargeted metabolomics. Results showed that the lifespan of C. elegans was significantly extended by 22.2% after high-dose AM treatment. AM improved the behavior and physiological functions of C. elegans by increasing the pharyngeal pumping rate, decreasing lipofuscin accumulation and the reactive oxygen species level, enhancing resistance to oxidative stress, and increasing the activities of superoxide dismutase and catalase. Transcriptome analysis showed that the pmk-1 gene (mitogen-activated protein kinase 1), which is involved in the MAPK signaling pathway, was the gene with the largest fold change after AM intervention. However, in the C. elegans pmk-1(km25) mutant, the beneficial effect of AM in improving nematode senescence disappeared. An untargeted metabolomics study showed that the levels of 4-hydroxyproline, rhamnose, and cysteine were increased after AM supplementation, and their extending effect on the lifespan and health-span of C. elegans were partly dependent on the pmk-1 gene. In conclusion, our results revealed that AM can promote the lifespan and health-span of C. elegans via the PMK-1 pathway, highlighting the potential of AM as a dietary supplement to delay aging.
期刊介绍:
Food & Function provides a unique venue for physicists, chemists, biochemists, nutritionists and other food scientists to publish work at the interface of the chemistry, physics and biology of food. The journal focuses on food and the functions of food in relation to health.