枸杞糖肽和异绿原酸 A 对 RAW264.7 细胞的协同免疫调节活性。

IF 3.5 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2024-10-22 DOI:10.1002/jsfa.13972

Ge Xu, Zhipeng Yu, Wenzhu Zhao

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引用次数: 0

摘要

背景：调节免疫系统以维持机体平衡已成为研究的重点，而多组分复合物的协同作用将有效提高免疫调节活性。本研究旨在探讨异绿原酸 A（IAA）与枸杞糖肽（LbGp）的相互作用及协同免疫调节活性：结果表明，分子间的非共价相互作用形成了 LbGp-IAA 复合物，结合率为 135.15 mg g-1。LbGp-IAA 复合物的形成改变了 LbGp 的构象，IAA 主要通过范德华力和氢键与 LbGp 结合。此外，LbGp-IAA 还能促进 RAW264.7 细胞的增殖。IAA与LbGp的相互作用对促进吞噬作用和一氧化氮、肿瘤坏死因子-α和白细胞介素-1β的表达有协同作用，从而提高了LbGp的免疫调节作用。此外，LbGp 和 IAA 的结合还能协同抑制脂多糖诱导的炎症反应：总之，IAA的结合增强了LbGp的免疫调节活性，协调了免疫反应，而没有引发炎症反应，这可能是由于IAA的结合改变了LbGp的空间结构。这些结果为研究糖肽与多酚的相互作用提供了新的视角。© 2024 化学工业协会。

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The synergistic immunomodulatory activity of Lycium barbarum glycopeptide and isochlorogenic acid A on RAW264.7 cells

BACKGROUND

Regulation of the immune system to maintain homeostasis in the organism has become a focus of research, and the synergistic effect of multi-component complexes will effectively improve the immunomodulatory activity. The present study aimed to investigate the interaction and synergistic immunomodulatory activity of isochlorogenic acid A (IAA) and Lycium barbarum glycopeptide (LbGp).

RESULTS

The results obtained indicated that non-covalent intermolecular interactions were employed to form the LbGp–IAA complex, with a binding ratio of 135.15 mg g⁻¹. The formation of LbGp–IAA complex altered the conformation of LbGp, and IAA was mainly bound to LbGp by van der Waals forces and hydrogen bonds. In addition, LbGp–IAA promoted the proliferation of RAW264.7 cells. The IAA and LbGp interaction had a synergistic effect on the promotion of phagocytosis and the expression of nitric oxide, tumor necrosis faction-α and interleukin-1β, which improved the immunomodulatory effect of LbGp. Furthermore, the combination of LbGp and IAA synergistically inhibited lipopolysaccharide-induced inflammatory response.

CONCLUSION

In summary, the binding of IAA enhanced the immunomodulatory activity of LbGp and coordinated the immune response, and did not trigger an inflammatory response, which was potentially attributed to the alteration of spatial structure of LbGp through the binding of IAA. The results provide new perspectives for the study of glycopeptide–polyphenol interactions. © 2024 Society of Chemical Industry.

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

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