胆汁酸紊乱和肠道屏障功能障碍与蛋鸡脂肪肝的形成有关

IF 3.8 1区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Poultry Science Pub Date : 2024-10-13 DOI:10.1016/j.psj.2024.104422

Lihua Zhao, Qiuyu Jiang, Jiaqi Lei, Jian Cui, Xianjie Pan, Yuan Yue, Bingkun Zhang

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引用次数: 0

摘要

脂肪肝的发病机制错综复杂。近年来，肠肝轴在脂肪肝发病过程中的作用已得到越来越多的认可。本研究旨在探讨胆汁酸信号转导和肠道屏障在脂肪肝发病机制中的作用。本研究共使用了 100 只 56 周龄的 "京挺 6 号 "蛋鸡，饲喂基础日粮至 60 周龄。实验结束后，根据肝脏脂肪变性程度挑选出 30 只个体。肝脏脂肪变性程度轻微（< 5 %）的蛋鸡被选为健康对照组，而肝脏脂肪变性程度严重（> 33 %）的蛋鸡被列为脂肪肝组。脂肪肝蛋鸡与健康对照组在体重、肝脏指数、腹脂比、饲料转化率（FCR）、白蛋白高度、Haugh单位和生化指标等方面存在显著差异。胆汁酸代谢组学研究结果显示，脂肪肝组与健康对照组的肝脏胆汁酸谱存在明显差异，脂肪肝组多种次级胆汁酸减少，表明胆汁酸代谢紊乱。此外，脂肪肝母鸡的肝脏和回肠末端法尼类固醇 X 受体（FXR）和胆汁酸转运相关基因的 mRNA 水平均显著下降。此外，脂肪肝蛋鸡的回肠隐窝深度、鹅口疮细胞数量和紧密连接相关蛋白的 mRNA 表达均显著下降，同时回肠通透性显著增加。这些发现共同表明，胆汁酸紊乱、FXR介导的信号传导受抑制以及肠道屏障功能受损是促进脂肪肝发生的潜在因素。这些发现表明，在不久的将来，调节胆汁酸和增强肠屏障功能可能会成为预防和治疗脂肪肝的新策略。

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Bile acid disorders and intestinal barrier dysfunction are involved in the development of fatty liver in laying hens

The pathogenesis of fatty liver is highly intricate. The role of the gut-liver axis in the development of fatty liver has gained increasing recognition in recent years. This study was conducted to explore the role of bile acid signaling and gut barrier in the pathogenesis of fatty liver. A total of 100 “Jing Tint 6” laying hens, 56-week-old, were used and fed basal diets until 60 weeks of age. At the end of the experiment, thirty individuals were selected based on the degree of hepatic steatosis. The hens with minimal hepatic steatosis (< 5 %) were chosen as healthy controls, while those with severe steatosis (> 33 %) in the liver were classified as the fatty liver group. Laying hens with fatty liver and healthy controls showed significant differences in body weight, liver index, abdominal fat ratio, feed conversion ratio (FCR), albumin height, Haugh unit, and biochemical indexes. The results of bile acid metabolomics revealed a clear separation in hepatic bile acid profiles between the fatty liver group and healthy controls, and multiple secondary bile acids were decreased in the fatty liver group, indicating disordered bile acid metabolism. Additionally, the mRNA levels of farnesoid X receptor (FXR) and genes related to bile acid transport were significantly decreased in both the liver and terminal ileum of hens with fatty liver. Moreover, the laying hens with fatty liver exhibited significant decreases in ileal crypt depth, the number of goblet cells, and the mRNA expression of tight junction-related proteins, alongside a significant increase in ileal permeability. Collectively, these findings suggest that disordered bile acids, suppressed FXR-mediated signaling, and impaired intestinal barrier function are potential factors promoting the development of fatty liver. These insights indicate that regulating bile acids and enhancing intestinal barrier function may become new preventive and therapeutic strategies for fatty liver in the near future.

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来源期刊

Poultry Science 农林科学-奶制品与动物科学

CiteScore

7.60

自引率

15.90%

发文量

审稿时长

94 days

期刊介绍： First self-published in 1921, Poultry Science is an internationally renowned monthly journal, known as the authoritative source for a broad range of poultry information and high-caliber research. The journal plays a pivotal role in the dissemination of preeminent poultry-related knowledge across all disciplines. As of January 2020, Poultry Science will become an Open Access journal with no subscription charges, meaning authors who publish here can make their research immediately, permanently, and freely accessible worldwide while retaining copyright to their work. Papers submitted for publication after October 1, 2019 will be published as Open Access papers. An international journal, Poultry Science publishes original papers, research notes, symposium papers, and reviews of basic science as applied to poultry. This authoritative source of poultry information is consistently ranked by ISI Impact Factor as one of the top 10 agriculture, dairy and animal science journals to deliver high-caliber research. Currently it is the highest-ranked (by Impact Factor and Eigenfactor) journal dedicated to publishing poultry research. Subject areas include breeding, genetics, education, production, management, environment, health, behavior, welfare, immunology, molecular biology, metabolism, nutrition, physiology, reproduction, processing, and products.