{"title":"具有有序大孔的手性金属有机框架薄膜用于蛋白质的对映选择性分析","authors":"Ji Yang, Qinyi Song, Tong Zhang, Yilun Yan, Chen Yuan, Yong Cui, Xiandeng Hou","doi":"10.1021/acs.analchem.4c03558","DOIUrl":null,"url":null,"abstract":"Chiral film-based sensors show great promise for discriminating between enantiomers due to their miniaturization and low power consumption. However, their practical use is hindered by the trade-off between enantioselectivity and mass transfer capability, especially concerning biomacromolecules such as proteins. In this work, we present an effective and straightforward method for creating highly organized macropores within crystalline chiral metal–organic framework (CMOF) films. This approach harnesses the shaping influence of a polystyrene nanosphere template and the crystallization induced by the liquid dielectric barrier discharge plasma. The resultant highly ordered macro–microporous structures improve mass diffusion and access to chiral active sites in the hierarchical CMOF films. Coupled with their inherent chirality, strong fluorescence emission, high crystallinity, and exceptional stability, these attributes endow these CMOF films with enhanced sensing capabilities for chiral molecules. Particularly, the macro–microporous structure facilitates efficient protein recognition, overcoming a significant challenge encountered by MOFs due to protein dimensions surpassing MOF pore sizes. These films exhibit increased enantioselectivity, better limits of detection, and wider linear ranges compared with purely microporous CMOF films. This study thus provides a powerful synthetic approach for hierarchical CMOF films, addressing the limitations of traditional thin film sensors and opening an avenue for efficient chiral sensing of large biomacromolecules.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":null,"pages":null},"PeriodicalIF":6.7000,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Chiral Metal–Organic Framework Films with Ordered Macropores for Enantioselective Analysis of Proteins\",\"authors\":\"Ji Yang, Qinyi Song, Tong Zhang, Yilun Yan, Chen Yuan, Yong Cui, Xiandeng Hou\",\"doi\":\"10.1021/acs.analchem.4c03558\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Chiral film-based sensors show great promise for discriminating between enantiomers due to their miniaturization and low power consumption. However, their practical use is hindered by the trade-off between enantioselectivity and mass transfer capability, especially concerning biomacromolecules such as proteins. In this work, we present an effective and straightforward method for creating highly organized macropores within crystalline chiral metal–organic framework (CMOF) films. This approach harnesses the shaping influence of a polystyrene nanosphere template and the crystallization induced by the liquid dielectric barrier discharge plasma. The resultant highly ordered macro–microporous structures improve mass diffusion and access to chiral active sites in the hierarchical CMOF films. Coupled with their inherent chirality, strong fluorescence emission, high crystallinity, and exceptional stability, these attributes endow these CMOF films with enhanced sensing capabilities for chiral molecules. Particularly, the macro–microporous structure facilitates efficient protein recognition, overcoming a significant challenge encountered by MOFs due to protein dimensions surpassing MOF pore sizes. These films exhibit increased enantioselectivity, better limits of detection, and wider linear ranges compared with purely microporous CMOF films. This study thus provides a powerful synthetic approach for hierarchical CMOF films, addressing the limitations of traditional thin film sensors and opening an avenue for efficient chiral sensing of large biomacromolecules.\",\"PeriodicalId\":27,\"journal\":{\"name\":\"Analytical Chemistry\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":6.7000,\"publicationDate\":\"2024-10-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Analytical Chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1021/acs.analchem.4c03558\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1021/acs.analchem.4c03558","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Chiral Metal–Organic Framework Films with Ordered Macropores for Enantioselective Analysis of Proteins
Chiral film-based sensors show great promise for discriminating between enantiomers due to their miniaturization and low power consumption. However, their practical use is hindered by the trade-off between enantioselectivity and mass transfer capability, especially concerning biomacromolecules such as proteins. In this work, we present an effective and straightforward method for creating highly organized macropores within crystalline chiral metal–organic framework (CMOF) films. This approach harnesses the shaping influence of a polystyrene nanosphere template and the crystallization induced by the liquid dielectric barrier discharge plasma. The resultant highly ordered macro–microporous structures improve mass diffusion and access to chiral active sites in the hierarchical CMOF films. Coupled with their inherent chirality, strong fluorescence emission, high crystallinity, and exceptional stability, these attributes endow these CMOF films with enhanced sensing capabilities for chiral molecules. Particularly, the macro–microporous structure facilitates efficient protein recognition, overcoming a significant challenge encountered by MOFs due to protein dimensions surpassing MOF pore sizes. These films exhibit increased enantioselectivity, better limits of detection, and wider linear ranges compared with purely microporous CMOF films. This study thus provides a powerful synthetic approach for hierarchical CMOF films, addressing the limitations of traditional thin film sensors and opening an avenue for efficient chiral sensing of large biomacromolecules.
期刊介绍:
Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.