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引用次数: 0
摘要
现有技术在实现高效 DNA 链置换反应(SDR)方面面临的一个主要挑战是,dsDNA 的连接和解离交替繁琐,导致动力学性能较差。在这项工作中,我们设计了一种新型多驱动 SDR,它由趾hold引发剂、链牵引和点击化学协同作用。入侵链(O)可与 dsDNA 中的基链(M)杂交,释放出置换链(P)。因此,与传统方法相比,P 的杂交速率和解离程度都有很大提高,并显示出接近 6 倍的期望置换率,为生物传感、临床诊断和 DNA 纳米技术的潜在应用提供了一种新的高效 SDR 策略。有鉴于此,我们将多驱动 SDR(MSDR)与无废物 DNA 多循环扩增相结合,构建了一个实用的生物传感平台,用于快速、超灵敏地电化学检测与癌症相关的 miRNA-21。利用所提出的 MSDR,目标触发的无废 DNA 多循环输出的大量 DNA 作为入侵链(O),可以在电极上高效释放信号探针(Fc)标记的置换链(P),从而获得低于 106.8 aM 的低检测限。
Efficient Multidriven Strand Displacement Reaction for Biosensing
A key challenge for achieving high-efficient DNA strand displacement reaction (SDR) with existing technologies is the inferior kinetic performance due to the alternately cumbersome conjunction and dissociation of dsDNA. In this work, a novel multidriven SDR collaborated by toehold initiator, strand towing, and click chemistry is engineered. The invasion strand (O) endows the hybridization with a basal strand (M) in dsDNA for releasing a displacement strand (P), which can be significantly boosted by the towing of a helper strand and impetus from the click reaction. Accordingly, the hybridization rate and dissociation extent of P can be largely improved and showed a desiring displacement rate close to 6-fold compared with the traditional method, providing a newly high-efficient SDR strategy for potential application in biosensing, clinical diagnostics, and DNA nanotechnology. In view of this, a practical biosensing platform by combining the multidriven SDR (MSDR) with waste-free DNA multi-cycle amplification is constructed for the rapid and ultrasensitive electrochemical detection of cancer-related miRNA-21. The substantial output DNA as an invasion strand (O) from target-triggered waste-free DNA multicycle can high-efficiently release a signal probe (Fc)-labeled displacement strand (P) on an electrode by using the proposed MSDR, obtaining a low detection limit below 106.8 aM.
期刊介绍:
Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.