Huijuan Tian, Shuqi Ding, Dan Zhang, Jinbin Wang, Mengting Hu, Kaizhi Yang, Ying Hao, Nan Qiao, Wentao Du, Ruifeng Li, Xudong Yang, Ruohang Xu
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Various analytical methods—correlation analysis, principal component analysis, subordinate function analysis, cluster analysis, stepwise discriminant analysis, and ridge regression analysis—were used to assess the seedling alkali tolerance of these maize germplasm resources. The physiological indices of six tested maize varieties were analyzed in greater detail. (3) The findings revealed complex correlations among traits, particularly strong negative associations between conductivity and root traits such as length, volume, surface area, diameter, and number of branches. The 15 evaluation indices were reduced to 7 principal components, explaining 77.89% of the variance. By applying affiliation functions and weights, we derived a comprehensive evaluation of maize seedling alkali tolerance. Notably, three germplasms—Liang Yu 99, Bi Xiang 638, and Gan Xin 2818—demonstrated significant comprehensive seedling alkali tolerance. Cluster analysis grouped the 65 maize germplasm resources into four distinct categories (I, II, III, and IV). The results of the cluster analysis were confirmed by multiclass stepwise discriminant analysis, which achieved a correct classification rate of 92.3% for 60 maize genotypes regarding alkalinity tolerance. Using principal component and ridge regression analyses, we formulated a regression equation for alkali tolerance: <i>D</i>-value = −1.369 + 0.002 * relative root volume + 0.003 * relative number of root forks + 0.006 * relative chlorophyll SPAD + 0.005 * relative stem thickness + 0.005 * relative plant height + 0.001 * relative conductivity + 0.002 * relative dry weight of underground parts. Under sodium bicarbonate stress, morphological indices and germination rates were significantly reduced, germination was inhibited, photosynthetic pigment levels in maize leaves decreased to varying degrees, and the activities of peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) significantly increased. Alkali stress markedly enhanced the antioxidant enzyme activities in maize varieties, with alkali-resistant varieties exhibiting a greater increase in antioxidant enzyme activities than alkali-sensitive varieties under such stress. (4) By screening for alkali tolerance in maize seedlings, the identified alkali-tolerant genotypes can be further utilized as suitable donor parents, thereby enhancing the use of alkali-tolerant germplasm resources and providing theoretical guidance for maize cultivation in saline-alkaline environments.</p>","PeriodicalId":54283,"journal":{"name":"Food and Energy Security","volume":null,"pages":null},"PeriodicalIF":4.0000,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fes3.70013","citationCount":"0","resultStr":"{\"title\":\"Sodium Bicarbonate Tolerance During Seedling Stages of Maize (Zea mays L.) 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Various analytical methods—correlation analysis, principal component analysis, subordinate function analysis, cluster analysis, stepwise discriminant analysis, and ridge regression analysis—were used to assess the seedling alkali tolerance of these maize germplasm resources. The physiological indices of six tested maize varieties were analyzed in greater detail. (3) The findings revealed complex correlations among traits, particularly strong negative associations between conductivity and root traits such as length, volume, surface area, diameter, and number of branches. The 15 evaluation indices were reduced to 7 principal components, explaining 77.89% of the variance. By applying affiliation functions and weights, we derived a comprehensive evaluation of maize seedling alkali tolerance. Notably, three germplasms—Liang Yu 99, Bi Xiang 638, and Gan Xin 2818—demonstrated significant comprehensive seedling alkali tolerance. 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Sodium Bicarbonate Tolerance During Seedling Stages of Maize (Zea mays L.) Lines
(1) Soil alkalinization and salinization represent a growing global challenge. Maize (Zea mays L.), with its relatively low tolerance to salt and alkali, is increasingly vulnerable to saline-alkali stress. Identifying maize genotypes that can withstand salinity and alkalinity is crucial to broaden the base of salt-alkali-tolerant maize germplasm. (2) In this study, we screened 65 maize germplasm resources for alkali stress using a 60 mM NaHCO3 solution. We measured fifteen morphological and physiological indices, including seedling height, stem thickness, and leaf area. Various analytical methods—correlation analysis, principal component analysis, subordinate function analysis, cluster analysis, stepwise discriminant analysis, and ridge regression analysis—were used to assess the seedling alkali tolerance of these maize germplasm resources. The physiological indices of six tested maize varieties were analyzed in greater detail. (3) The findings revealed complex correlations among traits, particularly strong negative associations between conductivity and root traits such as length, volume, surface area, diameter, and number of branches. The 15 evaluation indices were reduced to 7 principal components, explaining 77.89% of the variance. By applying affiliation functions and weights, we derived a comprehensive evaluation of maize seedling alkali tolerance. Notably, three germplasms—Liang Yu 99, Bi Xiang 638, and Gan Xin 2818—demonstrated significant comprehensive seedling alkali tolerance. Cluster analysis grouped the 65 maize germplasm resources into four distinct categories (I, II, III, and IV). The results of the cluster analysis were confirmed by multiclass stepwise discriminant analysis, which achieved a correct classification rate of 92.3% for 60 maize genotypes regarding alkalinity tolerance. Using principal component and ridge regression analyses, we formulated a regression equation for alkali tolerance: D-value = −1.369 + 0.002 * relative root volume + 0.003 * relative number of root forks + 0.006 * relative chlorophyll SPAD + 0.005 * relative stem thickness + 0.005 * relative plant height + 0.001 * relative conductivity + 0.002 * relative dry weight of underground parts. Under sodium bicarbonate stress, morphological indices and germination rates were significantly reduced, germination was inhibited, photosynthetic pigment levels in maize leaves decreased to varying degrees, and the activities of peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) significantly increased. Alkali stress markedly enhanced the antioxidant enzyme activities in maize varieties, with alkali-resistant varieties exhibiting a greater increase in antioxidant enzyme activities than alkali-sensitive varieties under such stress. (4) By screening for alkali tolerance in maize seedlings, the identified alkali-tolerant genotypes can be further utilized as suitable donor parents, thereby enhancing the use of alkali-tolerant germplasm resources and providing theoretical guidance for maize cultivation in saline-alkaline environments.
期刊介绍:
Food and Energy Security seeks to publish high quality and high impact original research on agricultural crop and forest productivity to improve food and energy security. It actively seeks submissions from emerging countries with expanding agricultural research communities. Papers from China, other parts of Asia, India and South America are particularly welcome. The Editorial Board, headed by Editor-in-Chief Professor Martin Parry, is determined to make FES the leading publication in its sector and will be aiming for a top-ranking impact factor.
Primary research articles should report hypothesis driven investigations that provide new insights into mechanisms and processes that determine productivity and properties for exploitation. Review articles are welcome but they must be critical in approach and provide particularly novel and far reaching insights.
Food and Energy Security offers authors a forum for the discussion of the most important advances in this field and promotes an integrative approach of scientific disciplines. Papers must contribute substantially to the advancement of knowledge.
Examples of areas covered in Food and Energy Security include:
• Agronomy
• Biotechnological Approaches
• Breeding & Genetics
• Climate Change
• Quality and Composition
• Food Crops and Bioenergy Feedstocks
• Developmental, Physiology and Biochemistry
• Functional Genomics
• Molecular Biology
• Pest and Disease Management
• Post Harvest Biology
• Soil Science
• Systems Biology