Enzytec™ 液体柠檬酸用于酶法检测部分食品和饮料中柠檬酸含量的验证:第一次行动 2024.02。

Markus Lacorn, Thomas Hektor
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引用次数: 0

摘要

背景:柠檬酸具有优异的酸化、抗氧化和防腐特性,因此被添加到各种食品和饮料中或成为其中的一种成分。为确保符合食品质量规格,必须对柠檬酸进行测量:验证 Enzytec™ 液体柠檬酸检测试剂盒在测定葡萄酒、果汁和番茄制品等食品和饮料中柠檬酸含量方面的性能:该试剂盒包含两种即用型成分,操作非常简单,适合自动化操作。柠檬酸在柠檬酸裂解酶的作用下裂解为草酰乙酸和乙酸。草酰乙酸在 L-苹果酸脱氢酶和还原型烟酰胺腺嘌呤二核苷酸(NADH)的作用下生成 L-苹果酸。由草酰乙酸自发生成的丙酮酸在 L-乳酸脱氢酶的作用下转化为 L-乳酸。消耗的 NADH 与转化的柠檬酸量相当,在 20 分钟内以 340 纳米波长进行测量:结果:该检测方法对柠檬酸具有特异性,无相关干扰。检测体积为 100 µL 时,检测限和定量限分别为 15 mg/L 和 40 mg/L。柠檬酸的线性测量范围为 40 至 1000 毫克/升。使用来自 FAPAS、NIST、LGC 的材料和德国葡萄酒分析师协会的两种对照葡萄酒进行了真实性评估。通过添加番茄酱、番茄糊、橙汁和碳酸饮料对基质干扰进行了评估,结果回收率约为 100%。对于提取基质,中间精度在 6.2% 至 8.5% 之间,而对于直接测量的基质,中间精度低于 4%。线性范围为 8 mg/L 至 5 g/L(取决于测试体积):结论:该方法可靠、准确,可用于人工和自动化应用。该方法已被批准为 AOAC 官方分析方法℠:该检测试剂盒的即用型成分自生产日期起保质期至少为 24 个月。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Validation of Enzytec™ Liquid Citric Acid for Enzymatic Determination of Citric Acid in Selected Foods and Beverages: First Action 2024.02.

Background: Due to its excellent acidifier, antioxidant, and preservative properties, citric acid is added to or is a constituent in a broad range of foods and beverages. Its measurement should be performed to assure the food quality specifications are met.

Objective: To validate the performance of the Enzytec™ Liquid Citric acid test kit for the determination in food and beverages such as wines, juices, and tomato products.

Methods: The kit contains two ready-to-use components, which makes handling very easy and suitable for automation. Citrate is cleaved into oxaloacetate and acetate by citrate lyase. Oxaloacetate reacts to L-malate by L-malate dehydrogenase and reduced nicotinamide adenine dinucleotide (NADH). Pyruvate, spontaneously formed from oxaloacetate, is converted by L-lactate dehydrogenase to L-lactate. The NADH consumed is equivalent to the amount of citric acid converted and is measured at a wavelength of 340 nm within 20 min.

Results: The test is specific to citric acid and shows no relevant interferences. Limit of detection and limit of quantification are 15 and 40 mg/L, respectively when using a test volume of 100 µL. The linear measurement range is from 40 to 1000 mg/L citric acid. Trueness was evaluated using materials from FAPAS, NIST, LGC, and two control wines from the German Wine Analysts. Matrix interference was evaluated by spiking tomato ketchup, tomato paste, orange juice, and carbonated beverages and resulted in recoveries around 100%. Intermediate precision is between 6.2 and 8.5% for matrixes with extraction and below 4% for matrixes measured directly. For automation, three applications with different test volumes and different measurement ranges were validated. Linearity is given from 8 mg/L up to 5 g/L (depending on the test volume).

Conclusions: The method is robust and accurate for manual and automated applications. The method was approved as an AOAC Official Method of Analysis℠.

Highlights: The ready-to-use components of the test kit have a shelf life of at least 24 months from the date of manufacture.

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