{"title":"转录组和代谢组分析揭示了中等浓度氯化钠处理对含油红酵母 Rhodosporidiobolus odoratus XQR 产生 β-胡萝卜素、香豌豆苷和香豌豆苷的积极影响","authors":"","doi":"10.1016/j.fochms.2024.100221","DOIUrl":null,"url":null,"abstract":"<div><div>Carotenoids, a family of lipid-soluble pigments, have garnered growing interest for their health-promoting benefits and are widely utilized in the food, feed, pharmaceutical, and cosmetic industries. <em>Rhodosporidiobolus odoratu</em>s, a representative oleaginous red yeast, is considered a promising alternative for producing high-value carotenoids including β-carotene, torulene, and torularhodin. Here, the impact of varying concentrations of NaCl treatments on carotenoid contents in <em>R. odoratus</em> XQR after 120 h of incubation was examined. The results indicated that, as compared to the control (59.37 μg/g<sub>dw</sub>), the synthesis of total carotenoids was significantly increased and entirely suppressed under low-to-moderate (0.25 mol/L: 68.06 μg/g<sub>dw</sub>, 0.5 mol/L: 67.62 μg/g<sub>dw</sub>, and 0.75 mol/L: 146.47 μg/g<sub>dw</sub>) and high (1.0, 1.25, and 1.5 mol/L: 0 μg/g<sub>dw</sub>) concentrations of NaCl treatments, respectively. Moreover, the maximum production of β-carotene (117.62 μg/g<sub>dw</sub>), torulene (21.81 μg/g<sub>dw</sub>), and torularhodin (7.04 μg/g<sub>dw</sub>) was achieved with a moderate concentration (0.75 mol/L) of NaCl treatment. Transcriptomic and metabolomic analyses suggested that the increase in β-carotene, torulene, and torularhodin production might be primarily attributed to the up-regulation of some key protein-coding genes involved in the terpenoid backbone biosynthesis (<em>atoB</em>, <em>HMGCS</em>, and <em>mvaD</em>), carotenoid biosynthesis (<em>crtYB</em> and <em>crtI</em>), and TCA cycle (<em>pckA</em>, <em>DLAT, pyc, MDH1</em>, <em>gltA</em>, <em>acnA</em>, <em>IDH1/2</em>, <em>IDH3</em>, <em>sucA</em>, <em>sucB</em>, <em>sucD</em>, <em>LSC1</em>, <em>SDHA</em>, and <em>fumA/fumB</em>). The present study not only demonstrates a viable method to concurrently increase the production of β-carotene, torulene, torularhodin, and total carotenoids in <em>R. odoratus</em> XQR, but it also establishes a molecular foundation for further enhancing their production through genetic engineering.</div></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":null,"pages":null},"PeriodicalIF":4.1000,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Transcriptomic and metabolomic analyses reveal the positive effect of moderate concentration of sodium chloride treatment on the production of β-carotene, torulene, and torularhodin in oleaginous red yeast Rhodosporidiobolus odoratus XQR\",\"authors\":\"\",\"doi\":\"10.1016/j.fochms.2024.100221\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Carotenoids, a family of lipid-soluble pigments, have garnered growing interest for their health-promoting benefits and are widely utilized in the food, feed, pharmaceutical, and cosmetic industries. <em>Rhodosporidiobolus odoratu</em>s, a representative oleaginous red yeast, is considered a promising alternative for producing high-value carotenoids including β-carotene, torulene, and torularhodin. Here, the impact of varying concentrations of NaCl treatments on carotenoid contents in <em>R. odoratus</em> XQR after 120 h of incubation was examined. The results indicated that, as compared to the control (59.37 μg/g<sub>dw</sub>), the synthesis of total carotenoids was significantly increased and entirely suppressed under low-to-moderate (0.25 mol/L: 68.06 μg/g<sub>dw</sub>, 0.5 mol/L: 67.62 μg/g<sub>dw</sub>, and 0.75 mol/L: 146.47 μg/g<sub>dw</sub>) and high (1.0, 1.25, and 1.5 mol/L: 0 μg/g<sub>dw</sub>) concentrations of NaCl treatments, respectively. Moreover, the maximum production of β-carotene (117.62 μg/g<sub>dw</sub>), torulene (21.81 μg/g<sub>dw</sub>), and torularhodin (7.04 μg/g<sub>dw</sub>) was achieved with a moderate concentration (0.75 mol/L) of NaCl treatment. Transcriptomic and metabolomic analyses suggested that the increase in β-carotene, torulene, and torularhodin production might be primarily attributed to the up-regulation of some key protein-coding genes involved in the terpenoid backbone biosynthesis (<em>atoB</em>, <em>HMGCS</em>, and <em>mvaD</em>), carotenoid biosynthesis (<em>crtYB</em> and <em>crtI</em>), and TCA cycle (<em>pckA</em>, <em>DLAT, pyc, MDH1</em>, <em>gltA</em>, <em>acnA</em>, <em>IDH1/2</em>, <em>IDH3</em>, <em>sucA</em>, <em>sucB</em>, <em>sucD</em>, <em>LSC1</em>, <em>SDHA</em>, and <em>fumA/fumB</em>). The present study not only demonstrates a viable method to concurrently increase the production of β-carotene, torulene, torularhodin, and total carotenoids in <em>R. odoratus</em> XQR, but it also establishes a molecular foundation for further enhancing their production through genetic engineering.</div></div>\",\"PeriodicalId\":34477,\"journal\":{\"name\":\"Food Chemistry Molecular Sciences\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.1000,\"publicationDate\":\"2024-08-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Food Chemistry Molecular Sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2666566224000285\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"FOOD SCIENCE & TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Chemistry Molecular Sciences","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2666566224000285","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
Transcriptomic and metabolomic analyses reveal the positive effect of moderate concentration of sodium chloride treatment on the production of β-carotene, torulene, and torularhodin in oleaginous red yeast Rhodosporidiobolus odoratus XQR
Carotenoids, a family of lipid-soluble pigments, have garnered growing interest for their health-promoting benefits and are widely utilized in the food, feed, pharmaceutical, and cosmetic industries. Rhodosporidiobolus odoratus, a representative oleaginous red yeast, is considered a promising alternative for producing high-value carotenoids including β-carotene, torulene, and torularhodin. Here, the impact of varying concentrations of NaCl treatments on carotenoid contents in R. odoratus XQR after 120 h of incubation was examined. The results indicated that, as compared to the control (59.37 μg/gdw), the synthesis of total carotenoids was significantly increased and entirely suppressed under low-to-moderate (0.25 mol/L: 68.06 μg/gdw, 0.5 mol/L: 67.62 μg/gdw, and 0.75 mol/L: 146.47 μg/gdw) and high (1.0, 1.25, and 1.5 mol/L: 0 μg/gdw) concentrations of NaCl treatments, respectively. Moreover, the maximum production of β-carotene (117.62 μg/gdw), torulene (21.81 μg/gdw), and torularhodin (7.04 μg/gdw) was achieved with a moderate concentration (0.75 mol/L) of NaCl treatment. Transcriptomic and metabolomic analyses suggested that the increase in β-carotene, torulene, and torularhodin production might be primarily attributed to the up-regulation of some key protein-coding genes involved in the terpenoid backbone biosynthesis (atoB, HMGCS, and mvaD), carotenoid biosynthesis (crtYB and crtI), and TCA cycle (pckA, DLAT, pyc, MDH1, gltA, acnA, IDH1/2, IDH3, sucA, sucB, sucD, LSC1, SDHA, and fumA/fumB). The present study not only demonstrates a viable method to concurrently increase the production of β-carotene, torulene, torularhodin, and total carotenoids in R. odoratus XQR, but it also establishes a molecular foundation for further enhancing their production through genetic engineering.