用纳米氧化铈提高葡萄(Vitis vinifera L., cv. Superior Seedless)的耐旱性:农艺学和分子研究

IF 3.9 2区 农林科学 Q1 HORTICULTURE
Selda Daler
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引用次数: 0

摘要

干旱胁迫对全球粮食安全构成重大威胁,通过生理、生化和分子变化影响葡萄的生长和发育。氧化铈纳米颗粒(CeO2 NPs)作为解决环境挑战的潜在方法最近受到了关注,但其在葡萄树中的应用仍未得到充分研究。本研究考察了叶面喷施 CeO2 NPs(浓度为 0、5、50 和 500 mg L-1)与葡萄树苗干旱胁迫(田间湿度为 30% 和 70%)之间的相互作用。在测试的不同浓度中,50 mg L-1 CeO2 NPs 能显著改善干旱胁迫下葡萄树幼苗的农艺性状(如芽长、叶面积、芽和根的干重)、光合参数(气孔导度、叶绿素 a 和叶绿素 b)和 RWC。同时,在干旱胁迫条件下,CeO2 NPs 能显著提高相容性溶质、SOD、CAT 和 APX 的活性。此外,CeO2 NPs 的存在减轻了干旱胁迫对葡萄树苗造成的损害,表现为 H2O2(32.63%)、EL(40.35%)和 MDA(50.63%)水平的降低。分子研究结果表明,CeO2 NPs 能显著改变干旱胁迫下的基因表达,使 VvCLH1、VvCu/ZnSOD、VvRD29A 和 VvRBOHA 基因的表达量分别降低 4.54 倍、27.73 倍、14.6 倍和 46.12 倍。这些研究结果表明,通过叶片施用 CeO2 NPs 可增强葡萄树苗对干旱诱导的氧化胁迫的抵抗力,其作用机制是通过酶和非酶抗氧化机制影响基因调控。因此,这些研究结果表明,CeO2 NPs可能是缓解葡萄树干旱胁迫的候选诱导剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Improving grapevine (Vitis vinifera L., cv. Superior Seedless) drought tolerance with cerium oxide nanoparticles: Agronomic and molecular insights

Drought stress poses a significant threat to global food security, impacting grapevine growth and development through physiological, biochemical, and molecular alterations. Cerium oxide nanoparticles (CeO2 NPs) have recently gained attention as a potential solution to environmental challenges, yet their application in grapevines remains understudied. This study examined the interaction between foliar-applied CeO2 NPs (at concentrations of 0, 5, 50, and 500 mg L−1) and drought stress (at 30% and 70% of field capacity humidity) in grapevine saplings. Among the different concentrations tested, 50 mg L−1 CeO2 NPs significantly improved the agronomic traits (e.g., shoot length, leaf area, shoot and root dry weights), photosynthetic parameters (stomatal conductance, chlorophyll a and chlorophyll b) and RWC of the grapevine saplings under drought stress. In parallel, CeO2 NPs significantly induced the activity of compatible solutes and SOD, CAT and APX under drought stress conditions. Moreover, the availability of CeO2 NPs alleviated drought stress-induced damage in grapevine saplings, as evidenced by decreased H2O2 (32.63%), EL (40.35%) and MDA (50.63%) levels. The molecular results revealed that CeO2 NPs caused significant changes in gene expression under drought stress by reducing the expression of the VvCLH1, VvCu/ZnSOD, VvRD29A and VvRBOHA genes by 4.54-fold, 27.73-fold, 14.6-fold and 46.12-fold, respectively. These findings suggest that CeO2 NPs applied via leaves enhance grapevine sapling resistance to drought-induced oxidative stress through cooperative enzymatic and nonenzymatic antioxidant mechanisms, influencing gene regulation. As a result, these findings revealed that CeO2 NPs could be promising elicitor candidates for alleviating drought stress in grapevines.

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来源期刊
Scientia Horticulturae
Scientia Horticulturae 农林科学-园艺
CiteScore
8.60
自引率
4.70%
发文量
796
审稿时长
47 days
期刊介绍: Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.
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