{"title":"用纳米氧化铈提高葡萄(Vitis vinifera L., cv. Superior Seedless)的耐旱性:农艺学和分子研究","authors":"Selda Daler","doi":"10.1016/j.scienta.2024.113606","DOIUrl":null,"url":null,"abstract":"<div><p>Drought stress poses a significant threat to global food security, impacting grapevine growth and development through physiological, biochemical, and molecular alterations. Cerium oxide nanoparticles (CeO<sub>2</sub> NPs) have recently gained attention as a potential solution to environmental challenges, yet their application in grapevines remains understudied. This study examined the interaction between foliar-applied CeO<sub>2</sub> NPs (at concentrations of 0, 5, 50, and 500 mg L<sup>−1</sup>) and drought stress (at 30% and 70% of field capacity humidity) in grapevine saplings. Among the different concentrations tested, 50 mg L<sup>−1</sup> CeO<sub>2</sub> NPs significantly improved the agronomic traits (e.g., shoot length, leaf area, shoot and root dry weights), photosynthetic parameters (stomatal conductance, chlorophyll a and chlorophyll <em>b</em>) and RWC of the grapevine saplings under drought stress. In parallel, CeO<sub>2</sub> NPs significantly induced the activity of compatible solutes and SOD, CAT and APX under drought stress conditions. Moreover, the availability of CeO<sub>2</sub> NPs alleviated drought stress-induced damage in grapevine saplings, as evidenced by decreased H<sub>2</sub>O<sub>2</sub> (32.63%), EL (40.35%) and MDA (50.63%) levels. The molecular results revealed that CeO<sub>2</sub> NPs caused significant changes in gene expression under drought stress by reducing the expression of the <em>VvCLH1, VvCu/ZnSOD, VvRD29A</em> and <em>VvRBOHA</em> genes by 4.54-fold, 27.73-fold, 14.6-fold and 46.12-fold, respectively. These findings suggest that CeO<sub>2</sub> NPs applied via leaves enhance grapevine sapling resistance to drought-induced oxidative stress through cooperative enzymatic and nonenzymatic antioxidant mechanisms, influencing gene regulation. As a result, these findings revealed that CeO<sub>2</sub> NPs could be promising elicitor candidates for alleviating drought stress in grapevines.</p></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"338 ","pages":"Article 113606"},"PeriodicalIF":3.9000,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Improving grapevine (Vitis vinifera L., cv. Superior Seedless) drought tolerance with cerium oxide nanoparticles: Agronomic and molecular insights\",\"authors\":\"Selda Daler\",\"doi\":\"10.1016/j.scienta.2024.113606\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Drought stress poses a significant threat to global food security, impacting grapevine growth and development through physiological, biochemical, and molecular alterations. Cerium oxide nanoparticles (CeO<sub>2</sub> NPs) have recently gained attention as a potential solution to environmental challenges, yet their application in grapevines remains understudied. This study examined the interaction between foliar-applied CeO<sub>2</sub> NPs (at concentrations of 0, 5, 50, and 500 mg L<sup>−1</sup>) and drought stress (at 30% and 70% of field capacity humidity) in grapevine saplings. Among the different concentrations tested, 50 mg L<sup>−1</sup> CeO<sub>2</sub> NPs significantly improved the agronomic traits (e.g., shoot length, leaf area, shoot and root dry weights), photosynthetic parameters (stomatal conductance, chlorophyll a and chlorophyll <em>b</em>) and RWC of the grapevine saplings under drought stress. In parallel, CeO<sub>2</sub> NPs significantly induced the activity of compatible solutes and SOD, CAT and APX under drought stress conditions. Moreover, the availability of CeO<sub>2</sub> NPs alleviated drought stress-induced damage in grapevine saplings, as evidenced by decreased H<sub>2</sub>O<sub>2</sub> (32.63%), EL (40.35%) and MDA (50.63%) levels. The molecular results revealed that CeO<sub>2</sub> NPs caused significant changes in gene expression under drought stress by reducing the expression of the <em>VvCLH1, VvCu/ZnSOD, VvRD29A</em> and <em>VvRBOHA</em> genes by 4.54-fold, 27.73-fold, 14.6-fold and 46.12-fold, respectively. These findings suggest that CeO<sub>2</sub> NPs applied via leaves enhance grapevine sapling resistance to drought-induced oxidative stress through cooperative enzymatic and nonenzymatic antioxidant mechanisms, influencing gene regulation. As a result, these findings revealed that CeO<sub>2</sub> NPs could be promising elicitor candidates for alleviating drought stress in grapevines.</p></div>\",\"PeriodicalId\":21679,\"journal\":{\"name\":\"Scientia Horticulturae\",\"volume\":\"338 \",\"pages\":\"Article 113606\"},\"PeriodicalIF\":3.9000,\"publicationDate\":\"2024-09-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Scientia Horticulturae\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0304423824007593\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"HORTICULTURE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Scientia Horticulturae","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0304423824007593","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"HORTICULTURE","Score":null,"Total":0}
Improving grapevine (Vitis vinifera L., cv. Superior Seedless) drought tolerance with cerium oxide nanoparticles: Agronomic and molecular insights
Drought stress poses a significant threat to global food security, impacting grapevine growth and development through physiological, biochemical, and molecular alterations. Cerium oxide nanoparticles (CeO2 NPs) have recently gained attention as a potential solution to environmental challenges, yet their application in grapevines remains understudied. This study examined the interaction between foliar-applied CeO2 NPs (at concentrations of 0, 5, 50, and 500 mg L−1) and drought stress (at 30% and 70% of field capacity humidity) in grapevine saplings. Among the different concentrations tested, 50 mg L−1 CeO2 NPs significantly improved the agronomic traits (e.g., shoot length, leaf area, shoot and root dry weights), photosynthetic parameters (stomatal conductance, chlorophyll a and chlorophyll b) and RWC of the grapevine saplings under drought stress. In parallel, CeO2 NPs significantly induced the activity of compatible solutes and SOD, CAT and APX under drought stress conditions. Moreover, the availability of CeO2 NPs alleviated drought stress-induced damage in grapevine saplings, as evidenced by decreased H2O2 (32.63%), EL (40.35%) and MDA (50.63%) levels. The molecular results revealed that CeO2 NPs caused significant changes in gene expression under drought stress by reducing the expression of the VvCLH1, VvCu/ZnSOD, VvRD29A and VvRBOHA genes by 4.54-fold, 27.73-fold, 14.6-fold and 46.12-fold, respectively. These findings suggest that CeO2 NPs applied via leaves enhance grapevine sapling resistance to drought-induced oxidative stress through cooperative enzymatic and nonenzymatic antioxidant mechanisms, influencing gene regulation. As a result, these findings revealed that CeO2 NPs could be promising elicitor candidates for alleviating drought stress in grapevines.
期刊介绍:
Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.