利用基于液相色谱-质谱联用技术的代谢组学和分子网络，比较分析芦笋不同溶剂提取物的代谢物特征

IF 3 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Phytochemical Analysis Pub Date : 2024-09-10 DOI:10.1002/pca.3446

Pfano W. Maphari, Mthokozisi B. C. Simelane, Ntakadzeni E. Madala, Msizi I. Mhlongo

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引用次数: 0

摘要

引言芦笋物种自然分布于世界各地，因其药理特性而闻名，可治疗各种疾病。本研究旨在利用不同的溶剂萃取方法对三种天门冬的代谢组进行分析和比较。方法采用基于超高效液相色谱-四极杆飞行时间质谱的代谢组学和分子网络方法，研究不同极性溶剂（乙酸乙酯、甲醇和氯仿）对从三种芦笋（Asparagus falcatus、Asparagus plumosus 和 Asparagus densiflorus 'Meyersii'）中提取代谢物和鉴定生物活性化合物的影响。结果多变量统计分析（主要是主成分分析）显示，三种溶剂和三种芦笋之间存在明显的差异，表明新陈代谢存在明显的不同。用不同溶剂萃取的三个物种共鉴定出 118 种代谢物，其中甲醇和氯仿萃取物比乙酸乙酯萃取物含有更多的代谢物。经鉴定，这些代谢物属于黄酮类、肉桂酸类、有机氧化合物类、类固醇类、脂肪酸类、苯类和甘油磷脂类化合物。此外，这些化合物类别在三个物种中的分布也不同，这表明比较物种之间存在化学/化合作用差异。建议使用氯仿和甲醇作为最佳溶剂，以从芦笋节片中获得高含量的植物化学化合物。

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Comparative metabolites profiling of different solvent extracts of Asparagus species cladodes using liquid chromatography–mass spectrometry‐based metabolomics and molecular networking

IntroductionAsparagus species are naturally distributed worldwide and are known for their pharmacological properties that offer cures for various ailments. However, the metabolic choreography of these Asparagus species is not well characterized, and the compounds contributing to their bioactivities remain unknown.ObjectiveThis study aimed to profile and compare the metabolomes of three Asparagus species cladodes using different solvent extractions.MethodsAn ultra‐high performance liquid chromatography–quadrupole time‐of‐flight mass spectrometry‐based metabolomics and molecular networking approach was used to study the effects of different solvents (ethyl acetate, methanol, and chloroform) with varying polarity on metabolites extraction and identification of bioactive compounds from three Asparagus species cladodes (Asparagus falcatus, Asparagus plumosus, and Asparagus densiflorus ‘Meyersii’).ResultsMultivariate statistical analyses (mainly principal component analysis) revealed a significant separation between the three solvents and the three species, indicating notable metabolic differences. A total of 118 metabolites were identified in the three species extracted with the different solvents, with methanolic and chloroform extracts containing more metabolites compared with ethyl acetate extracts. These metabolites were identified as belonging to the flavonoids, cinnamic acids, organooxygen compounds, steroids, fatty acids, benzenes, and glycerophospholipids compound classes. Furthermore, these compounds classes were differentially distributed among the three species, indicating chemical/chemotaxis differences between the compared species. Chloroform and methanol are recommended as the optimal solvents to obtain a high content of phytochemical compounds from Asparagus species cladodes.

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来源期刊

Phytochemical Analysis 生物-分析化学

CiteScore

6.00

自引率

6.10%

发文量

审稿时长

1.7 months

期刊介绍： Phytochemical Analysis is devoted to the publication of original articles concerning the development, improvement, validation and/or extension of application of analytical methodology in the plant sciences. The spectrum of coverage is broad, encompassing methods and techniques relevant to the detection (including bio-screening), extraction, separation, purification, identification and quantification of compounds in plant biochemistry, plant cellular and molecular biology, plant biotechnology, the food sciences, agriculture and horticulture. The Journal publishes papers describing significant novelty in the analysis of whole plants (including algae), plant cells, tissues and organs, plant-derived extracts and plant products (including those which have been partially or completely refined for use in the food, agrochemical, pharmaceutical and related industries). All forms of physical, chemical, biochemical, spectroscopic, radiometric, electrometric, chromatographic, metabolomic and chemometric investigations of plant products (monomeric species as well as polymeric molecules such as nucleic acids, proteins, lipids and carbohydrates) are included within the remit of the Journal. Papers dealing with novel methods relating to areas such as data handling/ data mining in plant sciences will also be welcomed.