基于设计质量原则开发和验证测定菊花中黄曲霉毒素的 DLLME-HPLC-FLD 方法

IF 3 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Phytochemical Analysis Pub Date : 2024-09-12 DOI:10.1002/pca.3445

Linlin Wu, Meixu Chen, Xinyi Huo, Qilin Xu, Xianggang Yin, Xiao Han Zhao, Yifeng Zhou, Jun Huang

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引用次数: 0

摘要

引言黄曲霉毒素是由曲霉菌产生的强致癌物质，对健康构成重大威胁，通常会污染菊花等草药产品。由于中草药基质的复杂性和不同样品中毒素含量的波动性，检测菊花中的黄曲霉毒素具有挑战性。本研究旨在根据设计质量原则，利用分散液-液微萃取结合高效液相色谱-荧光检测法，开发并优化检测菊花中黄曲霉毒素的新方法。采用蒙特卡罗模拟建立设计空间，并通过实验验证。结果所开发的方法在一定浓度范围内对黄曲霉毒素 B1、B2、G1 和 G2 具有良好的线性关系（R2 > 0.9991），回收率在 85.52% 至 102.01% 之间。本研究成功地建立了一种可靠有效的方法来检测大花蝉蜕中的黄曲霉毒素，强调了严格的贮藏条件对减少黄曲霉毒素污染的重要性。通过设计质量框架，该方法表现出了稳健性和较高的分析性能，适用于中草药产品的常规质量控制。

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Development and validation of a DLLME‐HPLC‐FLD method for determination of aflatoxins in Chrysanthemum morifolium based on quality by design principles

IntroductionAflatoxins, potent carcinogens produced by Aspergillus species, present significant health risks and commonly contaminate herbal products such as Chrysanthemum morifolium. Detecting these toxins in C. morifolium proves challenging due to the complex nature of the herbal matrix and the fluctuating levels of toxins found in different samples.ObjectivesThis study aimed to develop and optimize a novel method for the detection of aflatoxins in C. morifolium using dispersive liquid–liquid microextraction combined with high‐performance liquid chromatography‐fluorescence detection based on quality by design principles.MethodologyThe method involved determining critical method attributes and parameters through the Plackett–Burman design, followed by optimization using the Box–Behnken design. Monte Carlo simulation was employed to establish a design space, which was experimentally verified. Method validation was performed to confirm accuracy, precision, and stability.ResultsThe developed method exhibited excellent linearity (R2 > 0.9991) for aflatoxins B1, B2, G1, and G2 across a range of concentrations, with recovery rates between 85.52% and 102.01%. The validated method effectively quantified aflatoxins in C. morifolium under different storage conditions, highlighting the impact of temperature and storage time on aflatoxin production.ConclusionThis study successfully established a reliable and effective method for the detection of aflatoxins in C. morifolium, highlighting the importance of strict storage conditions to reduce aflatoxin contamination. Using a quality by design framework, the method demonstrated robustness and high analytical performance, making it suitable for routine quality control of herbal products.

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来源期刊

Phytochemical Analysis 生物-分析化学

CiteScore

6.00

自引率

6.10%

发文量

审稿时长

1.7 months

期刊介绍： Phytochemical Analysis is devoted to the publication of original articles concerning the development, improvement, validation and/or extension of application of analytical methodology in the plant sciences. The spectrum of coverage is broad, encompassing methods and techniques relevant to the detection (including bio-screening), extraction, separation, purification, identification and quantification of compounds in plant biochemistry, plant cellular and molecular biology, plant biotechnology, the food sciences, agriculture and horticulture. The Journal publishes papers describing significant novelty in the analysis of whole plants (including algae), plant cells, tissues and organs, plant-derived extracts and plant products (including those which have been partially or completely refined for use in the food, agrochemical, pharmaceutical and related industries). All forms of physical, chemical, biochemical, spectroscopic, radiometric, electrometric, chromatographic, metabolomic and chemometric investigations of plant products (monomeric species as well as polymeric molecules such as nucleic acids, proteins, lipids and carbohydrates) are included within the remit of the Journal. Papers dealing with novel methods relating to areas such as data handling/ data mining in plant sciences will also be welcomed.