{"title":"突尼斯小麦新品种 Aus26670 新的成株叶锈病抗性基因座的检测","authors":"Deepak Baranwal, Urmil Bansal, Harbans Bariana","doi":"10.1007/s40858-024-00675-z","DOIUrl":null,"url":null,"abstract":"<p>Transfer of leaf resistance genes into elite lines can assist in developing future wheat cultivars and mitigate economic losses caused by the leaf rust pathogen, <i>Puccinia triticina</i> Erikss<i>.</i> (Pt). Some previously reported leaf rust resistance QTL have been challenged by aggressive Pt pathotypes. This experiment aims to detect genetically diverse resistance QTL using a Tunisian landrace, Aus26670, which confers a high level of adult plant leaf rust resistance against Australian Pt pathotypes. One hundred nineteen F<sub>7</sub> recombinant inbred lines (RILs) were generated after crossing Aus26670 and a susceptible line Avocet ‘S’ (AvS). The Aus26670/AvS RIL population was evaluated against mixture of five Pt pathotypes under field conditions for two years. The same RIL population was also screened using three Pt pathotypes individually in the greenhouse under controlled conditions. Genetic analysis of the seedling leaf rust response against Pt104-2,3,6, (7) confirmed the presence of an all-stage resistance (ASR) gene, <i>Lr13</i>. The RIL population was genotyped using a targeted genotyping-by-sequencing (tGBS) assay, and <i>Lr13</i> was mapped in the 153.9–182.1 Mb region in chromosome 2BS. QTL analysis suggested the involvement of three genomic regions/adult plant resistance genes (APR) namely <i>QLr.sun-1BL/Lr46</i>, <i>QLr.sun-5DL</i> and <i>QLr.sun-7DS,</i> in controlling leaf rust resistance. Data of seedling assays, known marker survey, and comparison of genomic regions detected in this study with previously reported APR indicated the uniqueness of <i>QLr.sun-5DL</i> (559.7 Mb) and<i> QLr.sun-7DS</i> (11 Mb). Both QTL represent new additions to the APR toolbox.</p>","PeriodicalId":23354,"journal":{"name":"Tropical Plant Pathology","volume":"6 1","pages":""},"PeriodicalIF":2.5000,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Detection of new adult plant leaf rust resistance loci in a Tunisian wheat landrace Aus26670\",\"authors\":\"Deepak Baranwal, Urmil Bansal, Harbans Bariana\",\"doi\":\"10.1007/s40858-024-00675-z\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Transfer of leaf resistance genes into elite lines can assist in developing future wheat cultivars and mitigate economic losses caused by the leaf rust pathogen, <i>Puccinia triticina</i> Erikss<i>.</i> (Pt). Some previously reported leaf rust resistance QTL have been challenged by aggressive Pt pathotypes. This experiment aims to detect genetically diverse resistance QTL using a Tunisian landrace, Aus26670, which confers a high level of adult plant leaf rust resistance against Australian Pt pathotypes. One hundred nineteen F<sub>7</sub> recombinant inbred lines (RILs) were generated after crossing Aus26670 and a susceptible line Avocet ‘S’ (AvS). The Aus26670/AvS RIL population was evaluated against mixture of five Pt pathotypes under field conditions for two years. The same RIL population was also screened using three Pt pathotypes individually in the greenhouse under controlled conditions. Genetic analysis of the seedling leaf rust response against Pt104-2,3,6, (7) confirmed the presence of an all-stage resistance (ASR) gene, <i>Lr13</i>. The RIL population was genotyped using a targeted genotyping-by-sequencing (tGBS) assay, and <i>Lr13</i> was mapped in the 153.9–182.1 Mb region in chromosome 2BS. QTL analysis suggested the involvement of three genomic regions/adult plant resistance genes (APR) namely <i>QLr.sun-1BL/Lr46</i>, <i>QLr.sun-5DL</i> and <i>QLr.sun-7DS,</i> in controlling leaf rust resistance. Data of seedling assays, known marker survey, and comparison of genomic regions detected in this study with previously reported APR indicated the uniqueness of <i>QLr.sun-5DL</i> (559.7 Mb) and<i> QLr.sun-7DS</i> (11 Mb). Both QTL represent new additions to the APR toolbox.</p>\",\"PeriodicalId\":23354,\"journal\":{\"name\":\"Tropical Plant Pathology\",\"volume\":\"6 1\",\"pages\":\"\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2024-08-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Tropical Plant Pathology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1007/s40858-024-00675-z\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tropical Plant Pathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s40858-024-00675-z","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
Detection of new adult plant leaf rust resistance loci in a Tunisian wheat landrace Aus26670
Transfer of leaf resistance genes into elite lines can assist in developing future wheat cultivars and mitigate economic losses caused by the leaf rust pathogen, Puccinia triticina Erikss. (Pt). Some previously reported leaf rust resistance QTL have been challenged by aggressive Pt pathotypes. This experiment aims to detect genetically diverse resistance QTL using a Tunisian landrace, Aus26670, which confers a high level of adult plant leaf rust resistance against Australian Pt pathotypes. One hundred nineteen F7 recombinant inbred lines (RILs) were generated after crossing Aus26670 and a susceptible line Avocet ‘S’ (AvS). The Aus26670/AvS RIL population was evaluated against mixture of five Pt pathotypes under field conditions for two years. The same RIL population was also screened using three Pt pathotypes individually in the greenhouse under controlled conditions. Genetic analysis of the seedling leaf rust response against Pt104-2,3,6, (7) confirmed the presence of an all-stage resistance (ASR) gene, Lr13. The RIL population was genotyped using a targeted genotyping-by-sequencing (tGBS) assay, and Lr13 was mapped in the 153.9–182.1 Mb region in chromosome 2BS. QTL analysis suggested the involvement of three genomic regions/adult plant resistance genes (APR) namely QLr.sun-1BL/Lr46, QLr.sun-5DL and QLr.sun-7DS, in controlling leaf rust resistance. Data of seedling assays, known marker survey, and comparison of genomic regions detected in this study with previously reported APR indicated the uniqueness of QLr.sun-5DL (559.7 Mb) and QLr.sun-7DS (11 Mb). Both QTL represent new additions to the APR toolbox.
期刊介绍:
Tropical Plant Pathology is an international journal devoted to publishing a wide range of research on fundamental and applied aspects of plant diseases of concern to agricultural, forest and ornamental crops from tropical and subtropical environments.
Submissions must report original research that provides new insights into the etiology and epidemiology of plant disease as well as population biology of plant pathogens, host-pathogen interactions, physiological and molecular plant pathology, and strategies to promote crop protection.
The journal considers for publication: original articles, short communications, reviews and letters to the editor. For more details please check the submission guidelines.
Founded in 1976, the journal is the official publication of the Brazilian Phytopathology Society.