突尼斯小麦新品种 Aus26670 新的成株叶锈病抗性基因座的检测

IF 2.5 3区 农林科学 Q2 Agricultural and Biological Sciences
Deepak Baranwal, Urmil Bansal, Harbans Bariana
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引用次数: 0

摘要

将叶片抗性基因转入精英品系有助于开发未来的小麦栽培品种,并减轻叶锈病病原体 Puccinia triticina Erikss.(Pt)。之前报道的一些叶锈病抗性 QTL 受到了侵袭性 Pt 病型的挑战。本实验旨在利用突尼斯的一个陆地品种 Aus26670 来检测遗传多样性的抗性 QTL,该品种对澳大利亚的 Pt 病原型具有较高的成株叶锈病抗性。Aus26670 与易感品系 Avocet 'S' (AvS)杂交后,产生了 19 个 F7 重组近交系(RIL)。Aus26670/AvS RIL 群体在田间条件下针对五种 Pt 病型的混合物进行了为期两年的评估。同一 RIL 群体还在温室对照条件下单独使用三种 Pt 病原型进行了筛选。对幼苗叶锈病对 Pt104-2、3、6、(7)反应的遗传分析证实了全阶段抗性(ASR)基因 Lr13 的存在。利用定向基因分型测序法(tGBS)对 RIL 群体进行了基因分型,并将 Lr13 定位在 2BS 染色体的 153.9-182.1 Mb 区域。QTL分析表明,QLr.sun-1BL/Lr46、QLr.sun-5DL和QLr.sun-7DS这三个基因组区域/成株抗性基因(APR)参与了叶锈病抗性的控制。幼苗测定数据、已知标记调查以及本研究中检测到的基因组区域与之前报道的 APR 的比较表明,QLr.sun-5DL(559.7 Mb)和 QLr.sun-7DS(11 Mb)具有独特性。这两个 QTL 是 APR 工具箱中的新成员。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Detection of new adult plant leaf rust resistance loci in a Tunisian wheat landrace Aus26670

Detection of new adult plant leaf rust resistance loci in a Tunisian wheat landrace Aus26670

Transfer of leaf resistance genes into elite lines can assist in developing future wheat cultivars and mitigate economic losses caused by the leaf rust pathogen, Puccinia triticina Erikss. (Pt). Some previously reported leaf rust resistance QTL have been challenged by aggressive Pt pathotypes. This experiment aims to detect genetically diverse resistance QTL using a Tunisian landrace, Aus26670, which confers a high level of adult plant leaf rust resistance against Australian Pt pathotypes. One hundred nineteen F7 recombinant inbred lines (RILs) were generated after crossing Aus26670 and a susceptible line Avocet ‘S’ (AvS). The Aus26670/AvS RIL population was evaluated against mixture of five Pt pathotypes under field conditions for two years. The same RIL population was also screened using three Pt pathotypes individually in the greenhouse under controlled conditions. Genetic analysis of the seedling leaf rust response against Pt104-2,3,6, (7) confirmed the presence of an all-stage resistance (ASR) gene, Lr13. The RIL population was genotyped using a targeted genotyping-by-sequencing (tGBS) assay, and Lr13 was mapped in the 153.9–182.1 Mb region in chromosome 2BS. QTL analysis suggested the involvement of three genomic regions/adult plant resistance genes (APR) namely QLr.sun-1BL/Lr46, QLr.sun-5DL and QLr.sun-7DS, in controlling leaf rust resistance. Data of seedling assays, known marker survey, and comparison of genomic regions detected in this study with previously reported APR indicated the uniqueness of QLr.sun-5DL (559.7 Mb) and QLr.sun-7DS (11 Mb). Both QTL represent new additions to the APR toolbox.

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来源期刊
Tropical Plant Pathology
Tropical Plant Pathology PLANT SCIENCES-
CiteScore
4.50
自引率
4.00%
发文量
61
审稿时长
6-12 weeks
期刊介绍: Tropical Plant Pathology is an international journal devoted to publishing a wide range of research on fundamental and applied aspects of plant diseases of concern to agricultural, forest and ornamental crops from tropical and subtropical environments.  Submissions must report original research that provides new insights into the etiology and epidemiology of plant disease as well as population biology of plant pathogens, host-pathogen interactions, physiological and molecular plant pathology, and strategies to promote crop protection. The journal considers for publication: original articles, short communications, reviews and letters to the editor. For more details please check the submission guidelines. Founded in 1976, the journal is the official publication of the Brazilian Phytopathology Society.
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