基于深共晶溶剂的废弃无花果幼果多酚提取、表征和体外生物活性研究

IF 6 2区医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Antioxidants Pub Date : 2024-09-04 DOI:10.3390/antiox13091084

Qinqiu Zhang, Yue Peng, Yi Xu, Fan Li, Shuxiang Liu, Danka Bukvicki, Qing Zhang, Shang Lin, Miaomiao Wang, Tianyi Zhang, Dingtao Wu, Wen Qin

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引用次数: 0

摘要

(1) 背景：被丢弃的无花果幼果（DYFFs）会造成疏果和残果等资源浪费，目前还没有关于无花果幼果中酚类化合物与生物活性之间关系的研究 (2) 方法：采用不同的深共晶溶剂（DES）和80%乙醇制备DYFFs提取物，比较DYFFs提取物中多酚的提取效率和生物活性。(3) 结果：绿原酸、芦丁、8-C-叶黄素葡萄糖苷和表儿茶素是 DYFFs 中的主要多酚，尤其是绿原酸，含量为 2720-7980 mg/kg。阿魏酸、咖啡酸、表儿茶素、（+）-儿茶素、8-C-叶黄素葡萄糖苷、芦丁、橙皮素和绿原酸与体外抗氧化活性呈不同程度的相关性。此外，氯化胆碱-乙二醇（氯化胆碱：乙二醇=1:2）提取物中的总酚含量最高，为 8.88 毫克 GAE/g DW，各种 DES 和 80% 乙醇中所有定量分析的酚类化合物含量都很高。80% 乙醇和氯化胆碱（ChCl）溶剂系统的抗氧化性最强，氯化胆碱-尿素（氯化胆碱：尿素 = 1:2）提取物对 DYFFs 的抑制活性最高。(4) 结论：DESs 具有作为绿色溶剂的潜力，尤其是氯化胆碱溶剂体系，它有助于提取多酚。

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Extraction, Characterization, and In Vitro Biological Activity of Polyphenols from Discarded Young Fig Fruits Based on Deep Eutectic Solvents

(1) Background: Discarded young fig fruits (DYFFs) result in a waste of resources, such as sparse fruits and residual fruits, and there has been no research on the relationship between phenolic compounds and biological activity in DYFFs (2) Methods: Different deep eutectic solvents (DESs) and 80% ethanol were used to prepare DYFF extracts, and polyphenol extraction efficiency and bioactivities in the DYFFs extracts were compared. (3) Results: More than 1700 phytochemicals were identified in DYFFs, and thirteen of these typical phenolic compounds were analyzed quantitatively; chlorogenic acid, rutin, luteolin 8-C-glucoside, and epicatechin are the main polyphenols in DYFFs, especially chlorogenic acid with 2720–7980 mg/kg. Ferulic acid, caffeic acid, epicatechin, (+)-catechin, luteolin 8-C-glucoside, rutin, hesperetin, and chlorogenic acid showed different degrees of correlation with in vitro antioxidant activity. Moreover, the highest total phenol content found in the extracts of ChCl-Ethylene glycol (Choline chloride:Ethylene glycol = 1:2) was 8.88 mg GAE/g DW, and all quantitatively analyzed phenolic compounds had high levels in various DESs and 80% ethanol. The 80% ethanol and Choline chloride (ChCl) solvent system showed the greatest antioxidant properties, and the Choline chloride-Urea (Choline chloride: Urea = 1:2) extract of DYFFs exhibited the highest inhibitory activity. (4) Conclusions: DESs have demonstrated potential as promising green solvents, especially the ChCl solvent system, which facilitates the extraction of polyphenols.

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来源期刊

Antioxidants Biochemistry, Genetics and Molecular Biology-Physiology

CiteScore

10.60

自引率

11.40%

发文量

2123

审稿时长

16.3 days

期刊介绍： Antioxidants (ISSN 2076-3921), provides an advanced forum for studies related to the science and technology of antioxidants. It publishes research papers, reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.