膳食有机锌通过 RORγ 和表观遗传学调控改变暴露于高温应激的肉鸡回肠中的氧化基因

IF 6 2区医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Antioxidants Pub Date : 2024-09-04 DOI:10.3390/antiox13091079

Saber Y. Adam, Madesh Muniyappan, Hao Huang, Wael Ennab, Hao-Yu Liu, Abdelkareem A. Ahmed, Ming-an Sun, Tadelle Dessie, In Ho Kim, Yun Hu, Xugang Luo, Demin Cai

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引用次数: 0

摘要

热应激（HS）是肉鸡面临的一个重大问题，在家禽养殖这个充满活力的领域，肉鸡对全球肉类供应至关重要。热应激对回肠的影响及其对鸡体内氧化还原平衡基因的影响仍不清楚。我们假设，在热应激肉鸡的饲料中添加锌可提高它们对热应激的抵抗力。然而，本研究旨在探讨在 HS 条件下补充有机锌对肉鸡肠道组织学和 HS 指数基因调控的影响。本研究将 512 只雪山鸡分为四组：车辆组、HS 组、60 毫克/千克锌组和 HS + 60 毫克/千克锌组。研究结果表明，与 HS 组相比，补锌可使肉鸡回肠中的 VH 和 VH：CD 正向增加，而补锌和 HS+Zn 组肉鸡回肠中的 CD 和 VW 下降。与 HS 组相比，补锌可明显增加超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽（GSH）的含量，降低活性氧（ROS）和丙二醛（MDA）的酶活性。此外，与 HS 组相比，锌可明显提高 ATP、复合体 I、III 和 V 的相对酶活性。此外，与 HS 组相比，HS+Zn 组中酰基-CoA 合成酶长链家族成员 4（ACSL4）、乳酸转运体 3（LPCAT3）、过氧化物歧化酶（PRX）和转铁蛋白受体（TFRC）蛋白质水平的表达极度下调。与 HS 组相比，锌的供给明显降低了 RORγ、P300 和 SRC1 在 ACSL4、LPCAT3 和 PRX 靶基因座上的富集。与 HS 组相比，HS+Zn 组组蛋白活性标记 H3K9ac、H3K18ac、H3K27ac、H3K4me1 和 H3K18bhb 在 ACSL4 和 LPCAT3 基因座上的占有率明显下降。此外，与 HS 组相比，HS+Zn 组 ACSL4 和 LPCAT3 基因座上的 H3K9la 和 H3K18la 明显减少。本研究强调，有机锌是通过核受体RORγ调控和组蛋白修饰调节鸡回肠中氧化基因ACSL4、LPCAT3、PRX和TFRC的一种潜在策略。

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Dietary Organic Zinc Supplementation Modifies the Oxidative Genes via RORγ and Epigenetic Regulations in the Ileum of Broiler Chickens Exposed to High-Temperature Stress

Heat stress (HS) is a significant concern in broiler chickens, which is vital for global meat supply in the dynamic field of poultry farming. The impact of heat stress on the ileum and its influence on the redox homeostatic genes in chickens remains unclear. We hypothesized that adding zinc to the feed of heat-stressed broilers would improve their resilience to heat stress. However, this study aimed to explore the effects of organic zinc supplementation under HS conditions on broiler chickens’ intestinal histology and regulation of HS index genes. In this study, 512 Xueshan chickens were divided into four groups: vehicle, HS, 60 mg/kg zinc, and HS + 60 mg/kg zinc groups. Findings revealed that zinc supply positively increased the VH and VH: CD in the ileum of the broilers compared to the HS group, while CD and VW decreased in Zn and HS+Zn supplemented broilers. Zn administration significantly increased superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), and decreased the enzymatic activities of reactive oxygen species (ROS) and malondialdehyde (MDA) compared to the HS group. In addition, Zn administration significantly increased relative ATP, complex I, III, and V enzyme activity compared to the HS group. Furthermore, the expression of acyl-CoA synthetase long-chain family member 4 (ACSL4), lactate transporter 3 (LPCAT3), peroxiredoxin (PRX), and transferrin receptor (TFRC) in the protein levels was extremely downregulated in HS+Zn compared to the HS group. Zn supply significantly decreased the enrichment of RORγ, P300, and SRC1 at target loci of ACSL4, LPCAT3, and PRX compared to the HS group. The occupancies of histone active marks H3K9ac, H3K18ac, H3K27ac, H3K4me1, and H3K18bhb at the locus of ACSL4 and LPCAT3 were significantly decreased in HS+Zn compared to the HS group. Moreover, H3K9la and H3K18la at the locus of ACSL4 and LPCAT3 were significantly decreased in HS+Zn compared to the HS group. This study emphasizes that organic Zn is a potential strategy for modulating the oxidative genes ACSL4, LPCAT3, PRX, and TFRC in the ileum of chickens via nuclear receptor RORγ regulation and histone modifications.

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来源期刊

Antioxidants Biochemistry, Genetics and Molecular Biology-Physiology

CiteScore

10.60

自引率

11.40%

发文量

2123

审稿时长

16.3 days

期刊介绍： Antioxidants (ISSN 2076-3921), provides an advanced forum for studies related to the science and technology of antioxidants. It publishes research papers, reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.