大嘴鲈（Micropterus nigricans）反季节繁殖中睾丸的延迟繁殖、损伤和再生

IF 6 2区医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Antioxidants Pub Date : 2024-09-04 DOI:10.3390/antiox13091077

Kuo He, Yi Yang, Zhihong Li, Haoxiao Yan, Kaige Song, Qiao Liu, Liulan Zhao, Song Yang

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引用次数: 0

摘要

反季节繁殖是解决大口鲈鱼（LMB）等水产养殖物种鱼苗季节性短缺问题的有效方法，可实现全年生产。将养殖的大口鲈鱼置于长期低温条件下，可实现大口鲈鱼的反季节繁殖；但这会改变繁殖节律，影响其精子质量，导致繁殖效率下降。因此，研究反季节繁殖期间低温胁迫对睾丸的损伤及其相关机制等问题至关重要。在本实验中，我们通过比较繁殖节律、睾丸组织形态学、超微结构、抗氧化能力和细胞凋亡，评估了雌雄睾丸在此期间的变化。我们综合测量了来自三个相同处理的水泥池塘的鱲鱼和暴露在13-16 °C水温下的鱼类，以评估睾丸的变化。结果表明：（1）反季节繁殖延迟了鱲鱼精子的产生并促进了精子的再发育，各种激素水平随着时间的推移发生了变化（如LH、FSH和T）。(2）LMB精子头部质膜分离，中间线粒体肿胀。(3）抗氧化酶（cat、sod 和 gpx）表达水平上调，LMB 出现氧化应激。(4) 细胞凋亡基因（如 bax、bcl2 和 caspase3）的表达水平上调，淡季养殖导致 LMB 细胞凋亡。此外，线粒体凋亡途径的重要基因（bid、CYT-C）上调，表明LMB的精子凋亡可能是通过线粒体凋亡途径实现的。这些结果表明了 LMB 睾丸的延迟、损伤和再生。我们的研究结果为了解鱼类反季节繁殖过程中引发精子质量变化的分子机制提供了新的视角。

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Delayed Reproduction, Injury, and Regeneration of Testes in Out-of-Season Breeding of Largemouth Bass (Micropterus nigricans)

Out-of-season breeding is an effective method for addressing seasonal shortages of fry in aquaculture species such as largemouth bass (LMB) for year-round production. Off-season breeding of LMB can be achieved by subjecting breeding LMB to prolonged low-temperature conditions; however, this can alter reproductive rhythms, affecting the quality of their sperm and leading to a decrease in reproductive efficiency. Therefore, it is crucial to investigate issues such as the damage to the testes and the related mechanisms caused by low-temperature stress during out-of-season breeding. In this experiment, we assessed the changes in the testes during this time in LMB by comparing reproductive rhythms, testicular histomorphology, ultrastructure, antioxidant capacity and apoptosis. We synthesized measurements of LMB from three identically treated cement ponds and fish exposed to water temperatures of 13–16 °C to assess the changes in the testes. The results showed that (1) out-of-season reproduction delayed sperm production and promoted sperm redevelopment in LMB, various hormone levels have changed over time (e.g., LH, FSH, and T). (2) The head plasma membrane of LMB spermatozoa was separated, and the middle mitochondria were swollen. (3) The expression levels of antioxidant enzymes (cat, sod, and gpx) were upregulated, and oxidative stress occurred in LMB. (4) The expression levels of apoptosis genes (e.g., bax, bcl2, and caspase3) were upregulated, and apoptosis occurred in LMB due to off-season breeding. Moreover, important genes of the mitochondrial apoptosis pathway (bid, CYT-C) were upregulated, indicating that spermatozoan apoptosis in LMB was probably achieved through the mitochondrial apoptosis pathway. These results suggest the delays, damage, and regeneration of LMB testes. Our findings provide new insights into the molecular mechanisms that trigger changes in sperm quality during out-of-season breeding in fish.

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来源期刊

Antioxidants Biochemistry, Genetics and Molecular Biology-Physiology

CiteScore

10.60

自引率

11.40%

发文量

2123

审稿时长

16.3 days

期刊介绍： Antioxidants (ISSN 2076-3921), provides an advanced forum for studies related to the science and technology of antioxidants. It publishes research papers, reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.