关于曲霉胃蛋白酶在不同酶解和热处理条件下稳定葡萄汁和葡萄酒蛋白质的研究

IF 2.6 Q2 FOOD SCIENCE & TECHNOLOGY
Leif Seidel, Katrin Runkel, Wendell Albuquerque, Katharina Happel, Parviz Ghezellou, Bernhard Spengler, Holger Zorn, Martin Gand, Maximilian Freund, Frank Will, Ralf Schweiggert
{"title":"关于曲霉胃蛋白酶在不同酶解和热处理条件下稳定葡萄汁和葡萄酒蛋白质的研究","authors":"Leif Seidel, Katrin Runkel, Wendell Albuquerque, Katharina Happel, Parviz Ghezellou, Bernhard Spengler, Holger Zorn, Martin Gand, Maximilian Freund, Frank Will, Ralf Schweiggert","doi":"10.1021/acsfoodscitech.4c00308","DOIUrl":null,"url":null,"abstract":"Protein removal with aspergillopepsin in combination with short-time heating has recently emerged as an opportunity for winemakers to prevent protein haze in wine. In this study, we first treated a protein-rich must and wine with two different aspergillopepsin preparations along an identical control treatment without enzyme addition on a pilot plant scale. While treating wines was rather ineffective, treating musts was effective in reducing protein concentrations by up to 86%, leading to haze-stable wines according to the common heat test. Varying thermal loads in further experiments, a rather mild heat treatment of 65 °C for 30 s compared to those in earlier studies was found sufficient for enzymatic protein degradation. Treating must from a fungus-resistant variety (cv. Fidelio) was as successful as treating must from a blend of conventional varieties (cv. Gewürztraminer/Müller-Thurgau). Triangle sensory tests found no clear evidence of an effect on the quality by heat treatments with and without enzyme dosage.","PeriodicalId":72048,"journal":{"name":"ACS food science & technology","volume":null,"pages":null},"PeriodicalIF":2.6000,"publicationDate":"2024-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Investigations into the Protein Stabilization of Musts and Wines by Aspergillopepsin under Different Enzymatic and Thermal Treatments\",\"authors\":\"Leif Seidel, Katrin Runkel, Wendell Albuquerque, Katharina Happel, Parviz Ghezellou, Bernhard Spengler, Holger Zorn, Martin Gand, Maximilian Freund, Frank Will, Ralf Schweiggert\",\"doi\":\"10.1021/acsfoodscitech.4c00308\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Protein removal with aspergillopepsin in combination with short-time heating has recently emerged as an opportunity for winemakers to prevent protein haze in wine. In this study, we first treated a protein-rich must and wine with two different aspergillopepsin preparations along an identical control treatment without enzyme addition on a pilot plant scale. While treating wines was rather ineffective, treating musts was effective in reducing protein concentrations by up to 86%, leading to haze-stable wines according to the common heat test. Varying thermal loads in further experiments, a rather mild heat treatment of 65 °C for 30 s compared to those in earlier studies was found sufficient for enzymatic protein degradation. Treating must from a fungus-resistant variety (cv. Fidelio) was as successful as treating must from a blend of conventional varieties (cv. Gewürztraminer/Müller-Thurgau). Triangle sensory tests found no clear evidence of an effect on the quality by heat treatments with and without enzyme dosage.\",\"PeriodicalId\":72048,\"journal\":{\"name\":\"ACS food science & technology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2024-08-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS food science & technology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1021/acsfoodscitech.4c00308\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"FOOD SCIENCE & TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS food science & technology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1021/acsfoodscitech.4c00308","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

最近,使用曲霉胃蛋白酶去除蛋白质并结合短时间加热成为酿酒师防止葡萄酒中蛋白质混浊的一个机会。在这项研究中,我们首先用两种不同的曲霉胃蛋白酶制剂处理富含蛋白质的葡萄汁和葡萄酒,同时在试验工厂规模上进行不添加酶的相同对照处理。虽然对葡萄酒的处理效果不佳,但对葡萄汁的处理却能有效降低蛋白质浓度,降幅高达 86%,从而使葡萄酒在普通的热试验中具有稳定的雾度。在进一步的实验中,由于热负荷的不同,与之前的研究相比,65 °C 30 秒的温和热处理足以酶解蛋白质。处理抗真菌品种(Fidelio)的葡萄汁与处理传统品种(Gewürztraminer/Müller-Thurgau)混合的葡萄汁一样成功。三角感官测试没有发现明显的证据表明加酶和不加酶的热处理会影响葡萄汁的质量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Investigations into the Protein Stabilization of Musts and Wines by Aspergillopepsin under Different Enzymatic and Thermal Treatments

Investigations into the Protein Stabilization of Musts and Wines by Aspergillopepsin under Different Enzymatic and Thermal Treatments
Protein removal with aspergillopepsin in combination with short-time heating has recently emerged as an opportunity for winemakers to prevent protein haze in wine. In this study, we first treated a protein-rich must and wine with two different aspergillopepsin preparations along an identical control treatment without enzyme addition on a pilot plant scale. While treating wines was rather ineffective, treating musts was effective in reducing protein concentrations by up to 86%, leading to haze-stable wines according to the common heat test. Varying thermal loads in further experiments, a rather mild heat treatment of 65 °C for 30 s compared to those in earlier studies was found sufficient for enzymatic protein degradation. Treating must from a fungus-resistant variety (cv. Fidelio) was as successful as treating must from a blend of conventional varieties (cv. Gewürztraminer/Müller-Thurgau). Triangle sensory tests found no clear evidence of an effect on the quality by heat treatments with and without enzyme dosage.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
3.30
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信