微生物组和代谢组分析揭示植物乳杆菌-硫酸软骨素复合物的铅毒性缓解作用

IF 5.1 1区 农林科学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Food & Function Pub Date : 2024-09-10 DOI:10.1039/D4FO02815E
Feng Chen, Jiani Pan, Leilei Yu, Chuan Zhang, Jianxin Zhao, Fengwei Tian, Qixiao Zhai and Wei Chen
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引用次数: 0

摘要

铅(Pb)是一种对人体无生理功能的剧毒金属,会通过食物摄入在体内蓄积,并导致肠道微生物群紊乱和其他危害。在本研究中,我们考察了硫酸软骨素和植物乳杆菌 CCFM8661(CCFM8661+CS)组合对铅暴露小鼠的组织铅积累、肝脏和肾脏病理损伤、肠道微生物群和粪便代谢物的疗效。给暴露于铅的小鼠口服 CCFM8661+CS 可减少铅在肝脏、肾脏和骨骼组织中的积累(分别从 3.70、14.11 和 121.20 毫克/克湿组织降至 2.26、8.72 和 65.57 毫克/克湿组织),并提高肝脏和肾脏的总抗氧化能力、超氧化物歧化酶和谷胱甘肽。此外,肠道微生物组分析表明,CCFM8661+CS干预措施减轻了铅对肠道微生物组的干扰,改变了Faecalibaculum、Ruminococcaceae UCG 014、Anaerostipes和Enterorhabdus等细菌的丰度。非靶向代谢组学分析表明,CCFM8661+CS 可显著增加粪便中的肉桂酰甘氨酸、马尿酸和马尿醇(分别为基线的 31.24、28.77 和 20.13 倍);减少鸟嘌呤和 4-香豆酸(分别为基线的 0.30 和 0.09 倍);影响嘌呤和氨基酸代谢等途径。进一步分析表明,促进铅排泄和恢复受铅损害的肠道微生物群及其代谢可能是CCFM8661+CS减轻铅毒性的重要因素。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Lead toxicity mitigation effect of a Lactiplantibacillus plantarum–chondroitin sulfate complex revealed by microbiome and metabolomic analyses†

Lead toxicity mitigation effect of a Lactiplantibacillus plantarum–chondroitin sulfate complex revealed by microbiome and metabolomic analyses†

Lead (Pb) is a highly toxic metal with no physiological function in humans, accumulates in the body through food intake, and causes gut microbiome disorders and other hazards. In the present study, we examined the efficacy of a combination of chondroitin sulfate and Lactiplantibacillus plantarum CCFM8661 (CCFM8661 + CS) on tissue Pb accumulation and pathological damage to the liver and kidneys, gut microbiota, and fecal metabolites in Pb-exposed mice. Oral administration of CCFM8661 + CS to Pb-exposed mice reduced Pb accumulation in the liver, kidney, and bone tissues (from 3.70, 14.11 and 121.20 mg g−1 wet tissue to 2.26, 8.72 and 65.57 mg g−1 wet tissue, respectively) and increased total antioxidant capacity, superoxide dismutase, and glutathione in the liver and kidneys. Additionally, gut microbiome analysis showed that CCFM8661 + CS intervention attenuated Pb-induced perturbation in gut microbiota, altering the abundance of bacteria such as Faecalibaculum, Ruminococcaceae UCG 014, Anaerostipes, and Enterorhabdus. Untargeted metabolomics analyses showed that CCFM8661 + CS significantly increased cinnamoylglycine, hippuric acid, and equol (to 31.24, 28.77 and 20.13 times the baseline, respectively) and decreased guanine and 4-coumaric acid (0.30 and 0.09 times the baseline, respectively) in the feces, affecting pathways such as purine and amino acid metabolism. Further analyses showed that promoting Pb excretion and restoring the Pb-impaired gut microbiome and its metabolism may be important contributors to CCFM8661 + CS alleviation of Pb toxicity.

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来源期刊
Food & Function
Food & Function BIOCHEMISTRY & MOLECULAR BIOLOGY-FOOD SCIENCE & TECHNOLOGY
CiteScore
10.10
自引率
6.60%
发文量
957
审稿时长
1.8 months
期刊介绍: Food & Function provides a unique venue for physicists, chemists, biochemists, nutritionists and other food scientists to publish work at the interface of the chemistry, physics and biology of food. The journal focuses on food and the functions of food in relation to health.
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