膳食血红素富集棒状杆菌提取物通过重塑肠道微生物群对健康有益

IF 4.8 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY
{"title":"膳食血红素富集棒状杆菌提取物通过重塑肠道微生物群对健康有益","authors":"","doi":"10.1016/j.fbio.2024.105062","DOIUrl":null,"url":null,"abstract":"<div><p>We previously reported that a heme-enriched <em>Corynebacterium</em> extract (hSCP) alleviated weight gain and lowered subcutaneous fat mass in a murine model. To understand the mechanisms underlying the beneficial effects of dietary hSCP, we investigated the histological and microbiological changes in the guts of mice after hSCP administration in this study. Dietary hSCP lowered serum triglyceride and cholesterol levels and restrained adipose tissue expansion in mice fed a normal diet (ND) or a high-fat diet (HFD). The colonic mucosa was prominently lined with acidic and neutral mucin-enriched goblet cells and Muc2 transcription was increased by dietary hSCP in HFD-fed mice. Given the nutritional content of hSCP, microbial shifts triggered by dietary hSCP were dissected from the cecum and colon of the mice. Bacterial community compositions in the luminal contents were significantly reshaped by hSCP supplementation in HFD-fed mice and also differed between intestinal loci, that is, the cecum and colon. Dietary hSCP enriched short-chain fatty acid-producing bacterial genera in ND-fed mice, while attenuating other mucin-degrading bacteria in HFD-fed mice. The addition of hSCP to a co-culture of a defined microbiota community promoted <em>Lactobacillus gasseri</em> proliferation compared with nine other species. An <em>in vitro</em> culture medium supplemented with hSCP increased bile salt hydrolase activity and cholesterol assimilation by <em>L. gasseri</em>, which is reminiscent of the beneficial effects of <em>Lactobacillus</em> spp. as cholesterol-lowering probiotics. Our findings demonstrate the potential of hSCP as a dietary supplement with beneficial effects on lipid metabolism and gut health.</p></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":null,"pages":null},"PeriodicalIF":4.8000,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2212429224014925/pdfft?md5=88d9a89574782eebe783672b90c48b74&pid=1-s2.0-S2212429224014925-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Dietary heme-enriched Corynebacterium extract exerts health benefits by reshaping gut microbiota\",\"authors\":\"\",\"doi\":\"10.1016/j.fbio.2024.105062\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>We previously reported that a heme-enriched <em>Corynebacterium</em> extract (hSCP) alleviated weight gain and lowered subcutaneous fat mass in a murine model. To understand the mechanisms underlying the beneficial effects of dietary hSCP, we investigated the histological and microbiological changes in the guts of mice after hSCP administration in this study. Dietary hSCP lowered serum triglyceride and cholesterol levels and restrained adipose tissue expansion in mice fed a normal diet (ND) or a high-fat diet (HFD). The colonic mucosa was prominently lined with acidic and neutral mucin-enriched goblet cells and Muc2 transcription was increased by dietary hSCP in HFD-fed mice. Given the nutritional content of hSCP, microbial shifts triggered by dietary hSCP were dissected from the cecum and colon of the mice. Bacterial community compositions in the luminal contents were significantly reshaped by hSCP supplementation in HFD-fed mice and also differed between intestinal loci, that is, the cecum and colon. Dietary hSCP enriched short-chain fatty acid-producing bacterial genera in ND-fed mice, while attenuating other mucin-degrading bacteria in HFD-fed mice. The addition of hSCP to a co-culture of a defined microbiota community promoted <em>Lactobacillus gasseri</em> proliferation compared with nine other species. An <em>in vitro</em> culture medium supplemented with hSCP increased bile salt hydrolase activity and cholesterol assimilation by <em>L. gasseri</em>, which is reminiscent of the beneficial effects of <em>Lactobacillus</em> spp. as cholesterol-lowering probiotics. Our findings demonstrate the potential of hSCP as a dietary supplement with beneficial effects on lipid metabolism and gut health.</p></div>\",\"PeriodicalId\":12409,\"journal\":{\"name\":\"Food Bioscience\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.8000,\"publicationDate\":\"2024-09-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2212429224014925/pdfft?md5=88d9a89574782eebe783672b90c48b74&pid=1-s2.0-S2212429224014925-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Food Bioscience\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2212429224014925\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"FOOD SCIENCE & TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Bioscience","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2212429224014925","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

我们曾报道过一种富含血红素的棒状杆菌提取物(hSCP)能减轻小鼠模型的体重增加并降低皮下脂肪量。为了了解膳食 hSCP 的有益作用机制,我们在本研究中调查了服用 hSCP 后小鼠内脏的组织学和微生物学变化。在正常饮食(ND)或高脂饮食(HFD)喂养的小鼠中,膳食 hSCP 可降低血清甘油三酯和胆固醇水平,抑制脂肪组织扩张。在高脂饮食小鼠中,结肠粘膜显著衬有富含酸性和中性粘蛋白的鹅口疮细胞,饮食中的 hSCP 增加了 Muc2 的转录。鉴于 hSCP 的营养成分,研究人员对小鼠盲肠和结肠进行了解剖,发现饮食 hSCP 引发的微生物变化。补充 hSCP 后,HFD 小鼠肠腔内容物中的细菌群落组成发生了显著变化,而且不同肠道部位(即盲肠和结肠)的细菌群落组成也有所不同。膳食中的 hSCP 使 ND 饲料小鼠体内产生短链脂肪酸的细菌属更加丰富,而在 HFD 饲料小鼠体内则减少了其他降解粘蛋白的细菌。与其他九种细菌相比,在确定的微生物群落的联合培养中添加 hSCP 能促进加塞乳杆菌的增殖。添加了 hSCP 的体外培养基提高了加塞乳杆菌的胆盐水解酶活性和胆固醇同化能力,这让人联想到乳酸杆菌作为降胆固醇益生菌的有益作用。我们的研究结果证明了 hSCP 作为一种膳食补充剂对脂质代谢和肠道健康产生有益影响的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Dietary heme-enriched Corynebacterium extract exerts health benefits by reshaping gut microbiota

Dietary heme-enriched Corynebacterium extract exerts health benefits by reshaping gut microbiota

We previously reported that a heme-enriched Corynebacterium extract (hSCP) alleviated weight gain and lowered subcutaneous fat mass in a murine model. To understand the mechanisms underlying the beneficial effects of dietary hSCP, we investigated the histological and microbiological changes in the guts of mice after hSCP administration in this study. Dietary hSCP lowered serum triglyceride and cholesterol levels and restrained adipose tissue expansion in mice fed a normal diet (ND) or a high-fat diet (HFD). The colonic mucosa was prominently lined with acidic and neutral mucin-enriched goblet cells and Muc2 transcription was increased by dietary hSCP in HFD-fed mice. Given the nutritional content of hSCP, microbial shifts triggered by dietary hSCP were dissected from the cecum and colon of the mice. Bacterial community compositions in the luminal contents were significantly reshaped by hSCP supplementation in HFD-fed mice and also differed between intestinal loci, that is, the cecum and colon. Dietary hSCP enriched short-chain fatty acid-producing bacterial genera in ND-fed mice, while attenuating other mucin-degrading bacteria in HFD-fed mice. The addition of hSCP to a co-culture of a defined microbiota community promoted Lactobacillus gasseri proliferation compared with nine other species. An in vitro culture medium supplemented with hSCP increased bile salt hydrolase activity and cholesterol assimilation by L. gasseri, which is reminiscent of the beneficial effects of Lactobacillus spp. as cholesterol-lowering probiotics. Our findings demonstrate the potential of hSCP as a dietary supplement with beneficial effects on lipid metabolism and gut health.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Food Bioscience
Food Bioscience Biochemistry, Genetics and Molecular Biology-Biochemistry
CiteScore
6.40
自引率
5.80%
发文量
671
审稿时长
27 days
期刊介绍: Food Bioscience is a peer-reviewed journal that aims to provide a forum for recent developments in the field of bio-related food research. The journal focuses on both fundamental and applied research worldwide, with special attention to ethnic and cultural aspects of food bioresearch.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信