Umut Çelikoğlu, Emine Çelikoğlu, Muhammad Nauman Khan, Alevcan Kaplan
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Optical, thermal, and morphological structural analyses of ZnO-NPs and ZnO-MFs were conducted using Ultraviolet-Visible (UV-Vis) spectroscopy, Fourier Transform Infrared (FT-IR) spectroscopy, Thermogravimetric Analysis (TGA), and Field Emission Scanning Electron Microscopy (FE-SEM). Metabolic profiles of extracts from different plant parts were analyzed using Gas Chromatography-Mass Spectrometry (GC-MS) and supported by visualization of contents through Principal Component Analysis (PCA), hierarchical cluster analysis heatmaps, and Pearson correlation graphs. Interestingly, ZnO-NPs and ZnO-MFs exhibited strong antioxidant properties and demonstrated particularly potent antimicrobial activity against Micrococcus luteus NRRL B-4375, Escherichia coli ATCC 25922, and Candida albicans ATCC 10231 strains compared to standard antibiotics. Furthermore, ZnO-NPs and ZnO-MFs showed excellent plasmid DNA-cleavage activity of pBR322 with increasing doses. The photocatalytic performance of the synthesized ZnO-NPs and ZnO-MFs was evaluated for methylene blue (MB), congo red (CR), and safranin-O (SO) dyes, demonstrating remarkable color removal efficiency. Overall, the results provide a promising avenue for the green synthesis of ZnO-NPs and ZnO-MFs using I. oculus-christi L. inflorescence and pappus extracts, potentially revolutionizing biopharmaceutical and catalytic applications in these fields.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1875-1901"},"PeriodicalIF":3.5000,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Eco-benevolent synthesis of ZnO-NPs and ZnO-MFs from Inula oculus-christi L. 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引用次数: 0
摘要
由于近年来全球发生的变化，数以百万计的人正面临着挑战，甚至是威胁生命的疾病，如癌症和 COVID-19 大流行病等。这一现象促使研究人员开发和实施创新、环保的科学方法，将具有巨大技术潜力的学科（如纳米技术和药用植物）融合在一起。因此，本研究的重点是利用在正己烷和甲醇中制备的药用植物 I. oculus christi 的提取物作为新的生物还原剂和封端剂，通过简单、环保的化学方法合成锌纳米粒子（ZnO-NPs）和微花（ZnO-MFs）。使用紫外-可见光谱（UV-Vis）、傅立叶变换红外光谱（FT-IR）、热重分析（TGA）和场发射扫描电子显微镜（FE-SEM）对 ZnO-NPs 和 ZnO-MFs 进行了光学、热学和形态结构分析。利用气相色谱-质谱联用仪（GC-MS）分析了不同植物部位提取物的代谢概况，并通过主成分分析（PCA）、分层聚类分析热图和皮尔逊相关图对其内容进行了可视化。有趣的是，与标准抗生素相比，ZnO-NPs 和 ZnO-MFs 具有很强的抗氧化性，对黄体微球菌 NRRL B-4375、大肠杆菌 ATCC 25922 和白色念珠菌 ATCC 10231 株具有特别强的抗菌活性。此外，随着剂量的增加，ZnO-NPs 和 ZnO-MFs 对 pBR322 的质粒 DNA 也表现出卓越的清除活性。评估了合成的 ZnO-NPs 和 ZnO-MFs 对亚甲蓝（MB）、刚果红（CR）和黄芩苷-O（SO）染料的光催化性能，结果表明它们具有显著的除色效率。总之，研究结果为利用 I. oculus-christi L. 的花序和花被提取物绿色合成 ZnO-NPs 和 ZnO-MFs 提供了一条前景广阔的途径，有可能彻底改变这些领域的生物制药和催化应用。
Eco-benevolent synthesis of ZnO-NPs and ZnO-MFs from Inula oculus-christi L. (Asteraceae) with effective antioxidant, antimicrobial, DNA cleavage, and decolorization efficiencies.
As a result of the changes occurring globally in recent years, millions of people are facing challenging and even life-threatening diseases such as cancer and the COVID-19 pandemic, among others. This phenomenon has spurred researchers towards developing and implementing innovative and environmentally friendly scientific methods, merging disciplines with significant technological potential, such as nanotechnology with medicinal plants. Therefore, the focus of this research is to synthesize zinc nanoparticles (ZnO-NPs) and microflowers (ZnO-MFs) using extracts of the medicinal plant I. oculus christi prepared in n-hexane and methanol as new bioreduction and capping agents through a simple and environmentally friendly chemical approach. Optical, thermal, and morphological structural analyses of ZnO-NPs and ZnO-MFs were conducted using Ultraviolet-Visible (UV-Vis) spectroscopy, Fourier Transform Infrared (FT-IR) spectroscopy, Thermogravimetric Analysis (TGA), and Field Emission Scanning Electron Microscopy (FE-SEM). Metabolic profiles of extracts from different plant parts were analyzed using Gas Chromatography-Mass Spectrometry (GC-MS) and supported by visualization of contents through Principal Component Analysis (PCA), hierarchical cluster analysis heatmaps, and Pearson correlation graphs. Interestingly, ZnO-NPs and ZnO-MFs exhibited strong antioxidant properties and demonstrated particularly potent antimicrobial activity against Micrococcus luteus NRRL B-4375, Escherichia coli ATCC 25922, and Candida albicans ATCC 10231 strains compared to standard antibiotics. Furthermore, ZnO-NPs and ZnO-MFs showed excellent plasmid DNA-cleavage activity of pBR322 with increasing doses. The photocatalytic performance of the synthesized ZnO-NPs and ZnO-MFs was evaluated for methylene blue (MB), congo red (CR), and safranin-O (SO) dyes, demonstrating remarkable color removal efficiency. Overall, the results provide a promising avenue for the green synthesis of ZnO-NPs and ZnO-MFs using I. oculus-christi L. inflorescence and pappus extracts, potentially revolutionizing biopharmaceutical and catalytic applications in these fields.
期刊介绍：
Bioprocess and Biosystems Engineering provides an international peer-reviewed forum to facilitate the discussion between engineering and biological science to find efficient solutions in the development and improvement of bioprocesses. The aim of the journal is to focus more attention on the multidisciplinary approaches for integrative bioprocess design. Of special interest are the rational manipulation of biosystems through metabolic engineering techniques to provide new biocatalysts as well as the model based design of bioprocesses (up-stream processing, bioreactor operation and downstream processing) that will lead to new and sustainable production processes.
Contributions are targeted at new approaches for rational and evolutive design of cellular systems by taking into account the environment and constraints of technical production processes, integration of recombinant technology and process design, as well as new hybrid intersections such as bioinformatics and process systems engineering. Manuscripts concerning the design, simulation, experimental validation, control, and economic as well as ecological evaluation of novel processes using biosystems or parts thereof (e.g., enzymes, microorganisms, mammalian cells, plant cells, or tissue), their related products, or technical devices are also encouraged.
The Editors will consider papers for publication based on novelty, their impact on biotechnological production and their contribution to the advancement of bioprocess and biosystems engineering science. Submission of papers dealing with routine aspects of bioprocess engineering (e.g., routine application of established methodologies, and description of established equipment) are discouraged.
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