利用基因组学和蛋白质组学研究产脂酶微生物及其应用

IF 4.1 Q2 FOOD SCIENCE & TECHNOLOGY
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引用次数: 0

摘要

在生物技术应用中,脂肪酶被认为是使用最广泛、用途最广的酶,在生物催化过程中起着关键作用,主要由各种微生物产生。利用分子生物学技术,可以对天然资源进行细致筛选,找到负责产油的微生物菌群。脂肪酶是一种多功能生物催化剂。由于酶技术的快速发展及其在工业操作中的实用性,它们被用于各种生物转化反应,并受到广泛关注。本文介绍了真菌、细菌和酵母等微生物脂肪酶来源的最新研究成果,以及沉淀、免疫纯化和色谱分离等传统和现代纯化方法。此外,文章还探讨了反向胶束系统、水性两相系统(ATPS)和水性两相浮选(ATPF)等创新方法。文章论述了微生物脂肪酶在食品、纺织、皮革、化妆品、造纸、洗涤剂等多个领域的应用,同时还对产生脂肪酶的微生物进行了批判性分析。此外,它还强调了脂肪酶在生物传感器、生物柴油生产、茶叶加工、生物修复和消旋化方面的作用。这篇综述提供了在筛选能够产生脂肪酶的微生物物种的机制中使用全息技术的概念,同时也发现了潜在的应用领域。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Use of genomics & proteomics in studying lipase producing microorganisms & its application

In biotechnological applications, lipases are recognized as the most widely utilized and versatile enzymes, pivotal in biocatalytic processes, predominantly produced by various microbial species. Utilizing omics technology, natural sources can be meticulously screened to find microbial flora which are responsible for oil production. Lipases are versatile biocatalysts. They are used in a variety of bioconversion reactions and are receiving a lot of attention because of the quick development of enzyme technology and its usefulness in industrial operations. This article offers recent insights into microbial lipase sources, including fungi, bacteria, and yeast, alongside traditional and modern methods of purification such as precipitation, immunopurification and chromatographic separation. Additionally, it explores innovative methods like the reversed micellar system, aqueous two-phase system (ATPS), and aqueous two-phase flotation (ATPF). The article deals with the use of microbial lipases in a variety of sectors, including the food, textile, leather, cosmetics, paper, detergent, while also critically analyzing lipase-producing microbes. Moreover, it highlights the role of lipases in biosensors, biodiesel production, tea processing, bioremediation, and racemization. This review provides the concept of the use of omics technique in the mechanism of screening of microbial species those are capable of producing lipase and also find the potential applications.

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来源期刊
Food Chemistry Molecular Sciences
Food Chemistry Molecular Sciences Agricultural and Biological Sciences-Food Science
CiteScore
6.00
自引率
0.00%
发文量
83
审稿时长
82 days
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