Alba González-Fernández, Oscar Mencía-Ares, María José García-Iglesias, Máximo Petrocchi-Rilo, Rubén Miguélez-Pérez, César Bernardo Gutiérrez-Martín, Sonia Martínez-Martínez
{"title":"从西班牙养猪场采集的寄生璃泽氏菌(Glaesserella parasuis)分离株的毒性和抗菌特性。","authors":"Alba González-Fernández, Oscar Mencía-Ares, María José García-Iglesias, Máximo Petrocchi-Rilo, Rubén Miguélez-Pérez, César Bernardo Gutiérrez-Martín, Sonia Martínez-Martínez","doi":"10.3390/antibiotics13080741","DOIUrl":null,"url":null,"abstract":"<p><p><i>Glaesserella</i> (<i>Haemophilus</i>) <i>parasuis</i>, the causative agent of Glässer's disease, is present in most pig farms as an early colonizer of the upper respiratory tract. It exhibits remarkable variability in virulence and antimicrobial resistance (AMR), with virulent strains capable of inducing respiratory or systemic disease. This study aimed to characterize the virulence and the AMR profiles in 65 <i>G. parasuis</i> isolates recovered from Spanish swine farms. Virulence was assessed using multiplex leader sequence (LS)-PCR targeting <i>vtaA</i> genes, with all isolates identified as clinical (presumed virulent). Pathotyping based on ten pangenome genes revealed the virulent HPS_22970 as the most frequent (83.1%). Diverse pathotype profiles were observed, with 29 unique gene combinations and two isolates carrying only potentially non-virulent pangenome genes. AMR phenotyping showed widespread resistance, with 63.3% classified as multidrug resistant, and high resistance to clindamycin (98.3%) and tylosin (93.3%). A very strong association was found between certain pathotype genes and AMR phenotypes, notably between the virulent HPS_22970 and tetracycline resistance (<i>p</i> < 0.001; Φ = 0.58). This study reveals the wide diversity and complexity of <i>G. parasuis</i> pathogenicity and AMR phenotype, emphasizing the need for the targeted characterization of clinical isolates to ensure appropriate antimicrobial treatments and the implementation of prophylactic measures against virulent strains.</p>","PeriodicalId":54246,"journal":{"name":"Antibiotics-Basel","volume":null,"pages":null},"PeriodicalIF":4.3000,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11350796/pdf/","citationCount":"0","resultStr":"{\"title\":\"Virulence and Antimicrobial Resistance Characterization of <i>Glaesserella parasuis</i> Isolates Recovered from Spanish Swine Farms.\",\"authors\":\"Alba González-Fernández, Oscar Mencía-Ares, María José García-Iglesias, Máximo Petrocchi-Rilo, Rubén Miguélez-Pérez, César Bernardo Gutiérrez-Martín, Sonia Martínez-Martínez\",\"doi\":\"10.3390/antibiotics13080741\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><i>Glaesserella</i> (<i>Haemophilus</i>) <i>parasuis</i>, the causative agent of Glässer's disease, is present in most pig farms as an early colonizer of the upper respiratory tract. 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Virulence and Antimicrobial Resistance Characterization of Glaesserella parasuis Isolates Recovered from Spanish Swine Farms.
Glaesserella (Haemophilus) parasuis, the causative agent of Glässer's disease, is present in most pig farms as an early colonizer of the upper respiratory tract. It exhibits remarkable variability in virulence and antimicrobial resistance (AMR), with virulent strains capable of inducing respiratory or systemic disease. This study aimed to characterize the virulence and the AMR profiles in 65 G. parasuis isolates recovered from Spanish swine farms. Virulence was assessed using multiplex leader sequence (LS)-PCR targeting vtaA genes, with all isolates identified as clinical (presumed virulent). Pathotyping based on ten pangenome genes revealed the virulent HPS_22970 as the most frequent (83.1%). Diverse pathotype profiles were observed, with 29 unique gene combinations and two isolates carrying only potentially non-virulent pangenome genes. AMR phenotyping showed widespread resistance, with 63.3% classified as multidrug resistant, and high resistance to clindamycin (98.3%) and tylosin (93.3%). A very strong association was found between certain pathotype genes and AMR phenotypes, notably between the virulent HPS_22970 and tetracycline resistance (p < 0.001; Φ = 0.58). This study reveals the wide diversity and complexity of G. parasuis pathogenicity and AMR phenotype, emphasizing the need for the targeted characterization of clinical isolates to ensure appropriate antimicrobial treatments and the implementation of prophylactic measures against virulent strains.
Antibiotics-BaselPharmacology, Toxicology and Pharmaceutics-General Pharmacology, Toxicology and Pharmaceutics
CiteScore
7.30
自引率
14.60%
发文量
1547
审稿时长
11 weeks
期刊介绍:
Antibiotics (ISSN 2079-6382) is an open access, peer reviewed journal on all aspects of antibiotics. Antibiotics is a multi-disciplinary journal encompassing the general fields of biochemistry, chemistry, genetics, microbiology and pharmacology. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on the length of papers.