膜荚黄芪(Astragalus membranaceus Fisch.

IF 6 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Saba Shahrivari-Baviloliaei, Ilkay Erdogan Orhan, Nurten Abaci Kaplan, Agnieszka Konopacka, Krzysztof Waleron, Alina Plenis, Agnieszka Viapiana
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引用次数: 0

摘要

黄芪(Astragalus membranaceus Fisch.因此,本研究旨在利用化学计量学方法计算膜荚黄芪水甲醇提取物和水提取物的酚类成分、抗氧化性、抗菌性以及酶抑制性 [乙酰胆碱酯酶 (AChE)、丁酰胆碱酯酶 (BChE) 和酪氨酸酶 (TYR)]。使用高效液相色谱法(HPLC)测定了十种酚类化合物,发现两种提取物中只有槲皮素的含量高于 80 µg/g DW。此外,在 DPPH、FRAP、ABTS 和 CUPRAC 试验中,含有美国松散根的样品的抗氧化活性最高。A. membranaceus 提取物对金黄色葡萄球菌的抑制区直径在 10 到 22 毫米之间,而对大肠杆菌则没有抑制区。膜草提取物对 TYR 的抑制率低于 40%,在测试的提取物中,只有两种样品能够抑制 BChE,其 IC50 值高于 30 µg/mL。相关分析表明,它们的酚类成分与抗氧化活性之间存在高度正相关关系。总之,研究结果证实,膜草属植物的商业样本可以作为天然抗氧化剂的重要膳食来源。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Characterization of Phenolic Profile and Biological Properties of Astragalus membranaceus Fisch. ex Bunge Commercial Samples.

Astragalus membranaceus Fisch. ex Bunge (syn. Astragalus mongholicus Bunge) is one of the notable medicinal and food plants. Therefore, the aim of this study was to calculate the phenolic composition and antioxidant, antimicrobial, as well as enzyme inhibitory [acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and tyrosinase (TYR)] activities with chemometric approaches of the hydromethanolic and water extracts of commercial A. membranaceus samples. Ten individual phenolic compounds were determined using high-performance liquid chromatography (HPLC), and only quercetin was found at a level of above 80 µg/g DW in both extracts. Moreover, the highest antioxidant activity in DPPH, FRAP, ABTS, and CUPRAC assays was found in the sample containing the roots in loose form from USA. A. membranaceus extracts displayed the inhibition zone diameters within the range from 10 to 22 mm antimicrobial activity against S. aureus, while there were no inhibition zones in any extracts in case of E. coli. The extracts of A. membranaceous showed an inhibition rate below 40% against TYR, and among tested extracts, only two samples were able to inhibit BChE with IC50 values of above 30 µg/mL. Correlation analysis showed a highly positive relationship between their phenolic composition and antioxidant activity. Concluding, the obtained results confirmed that A. membranaceus commercial samples could be an important dietary source of natural antioxidants.

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来源期刊
Antioxidants
Antioxidants Biochemistry, Genetics and Molecular Biology-Physiology
CiteScore
10.60
自引率
11.40%
发文量
2123
审稿时长
16.3 days
期刊介绍: Antioxidants (ISSN 2076-3921), provides an advanced forum for studies related to the science and technology of antioxidants. It publishes research papers, reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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