利用细胞病理学进行口腔癌筛查:贝塞斯达分类法高级提案。

IF 1.2 4区 医学 Q4 CELL BIOLOGY
Cytopathology Pub Date : 2024-08-20 DOI:10.1111/cyt.13427
Tatiana Wannmacher Lepper, Renata Ferreira Prigol, Ana Laura Ferrares Espinosa, Igor Cavalcante Guedes, Maikel Maciel Rönnau, Natália Batista Daroit, Alex Nogueira Haas, Fernanda Visioli, Manuel Menezes de Oliveira Neto, Pantelis Varvaki Rados
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引用次数: 0

摘要

背景:口腔癌筛查需要能够检测出临床表现前变化的分析。口腔细胞病理学研究作为口腔癌筛查的一种方法,已经显示出良好的效果。本研究旨在评估使用巴氏染色法对细胞病理学进行定性定量分析的效果:评估了四个实验组:对照组(CG)、暴露于致癌物质组(EG)、有上皮发育不良和无上皮发育不良的潜在恶性疾病组(D-OPMDG 和 ND-OPMDG)以及口腔鳞状细胞癌组(OSCCG)。使用 Cytobrush® 和传统脱落细胞学方法采集口腔涂片:对 143 人的口腔巴氏涂片的 8100 张图像进行了分析。从质量上看,非lesional组的疑似病例极少(CG为20%,EG为5%),而在OPMD组中,识别改变细胞的能力很低(D-OPMDG为40%,ND-OPMDG为0%)。相反,OSCCG 的准确率达到了显著的 100%。从数量上看,在 OPMDG 中观察到的无核细胞比例较高,中间细胞比例较低;在 OSCCG 中观察到的副基底/可疑细胞比例较高。当副基底/可疑细胞数超过 8/100 时,是提高检测疑似恶性肿瘤准确性的最佳阈值。细胞形态计量学分析表明,OSCCG 中的细胞核/细胞质比值(N/C)较高,当表现出这一升高比值的细胞超过 17% 时,即为最佳临界点,可提高鉴别疑似恶性肿瘤的准确性:结论:建议对口腔改良贝塞斯达系统中的细胞形态学和定量分析进行更新,在风险分类中加入客观标准,从而提高口腔癌筛查水平。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Oral cancer screening using cytopathology: An advanced proposal with the Bethesda classification.

Background: Oral cancer screening requires analysis capable of detecting changes preceding the clinical manifestation. Oral cytopathology studies as an oral cancer screening have shown promising results. This study aims to evaluate the use of cytopathology in a quali-quantitative analysis using Papanicoloau staining.

Methods: Four experimental groups were evaluated: control (CG), exposed to carcinogens (EG), potentially malignant disorder with and without epithelial dysplasia (D-OPMDG and ND-OPMDG) and oral squamous cell carcinoma (OSCCG). Oral smears were collected using a Cytobrush® and conventional exfoliative cytology.

Results: Oral Papanicolaou smears from 143 individuals were analysed in 8100 images. Qualitatively, non-lesional groups exhibited minimal suspected cases (20% in CG and 5% in EG), in the OPMD groups the ability to identify altered cells was low (40% in D-OPMDG and 0% in ND-OPMDG). Conversely, a notable 100% accuracy was achieved in the OSCCG. Quantitatively, a higher percentage of anucleated and a lower percentage of intermediate cells were observed in the OPMDG; a higher proportion of parabasal/suspicious cells was observed in OSCCG. The optimal threshold for improved accuracy in detecting suspected malignancies occurs when the count of parabasal/suspicious cells exceeds 8/100 cells examined. Cytomorphometric analysis revealed a higher nucleus/cytoplasm ratio (N/C) in OSCCG, with a best cutoff point indicating enhanced accuracy in discerning suspected malignancies when exceeding 17% of cells exhibiting this elevated ratio.

Conclusion: It is possible to suggest updates in cytomorphometric and quantitative analysis in the modified Bethesda system for the oral cavity including objective criteria in the risk classification, therefore improving oral cancer screening.

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来源期刊
Cytopathology
Cytopathology 生物-病理学
CiteScore
2.30
自引率
15.40%
发文量
107
审稿时长
6-12 weeks
期刊介绍: The aim of Cytopathology is to publish articles relating to those aspects of cytology which will increase our knowledge and understanding of the aetiology, diagnosis and management of human disease. It contains original articles and critical reviews on all aspects of clinical cytology in its broadest sense, including: gynaecological and non-gynaecological cytology; fine needle aspiration and screening strategy. Cytopathology welcomes papers and articles on: ultrastructural, histochemical and immunocytochemical studies of the cell; quantitative cytology and DNA hybridization as applied to cytological material.
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