一种天然查耳酮白豆蔻素通过与受体竞争性结合抑制芳香烃受体的转化

IF 4.5 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY
Tianshun Zhang, Chao He, Shieru Ota, Tomoya Kitakaze, Ryoichi Yamaji, Sayuri Shimazu, Yoko Yamashita, Hitoshi Ashida
{"title":"一种天然查耳酮白豆蔻素通过与受体竞争性结合抑制芳香烃受体的转化","authors":"Tianshun Zhang,&nbsp;Chao He,&nbsp;Shieru Ota,&nbsp;Tomoya Kitakaze,&nbsp;Ryoichi Yamaji,&nbsp;Sayuri Shimazu,&nbsp;Yoko Yamashita,&nbsp;Hitoshi Ashida","doi":"10.1002/mnfr.202400185","DOIUrl":null,"url":null,"abstract":"<div>\n \n <section>\n \n <h3> Scope</h3>\n \n <p>Chalcones are widely present in most plants and have various health beneficial functions. This study investigates the suppressive effect of 13 natural and synthetic chalcones on transformation of aryl hydrocarbon receptor (AhR) induced by 2,3,7,8-tetrachlorodibenzo-<i>p</i>-dioxin (TCDD) and 3-methylcholanthrene (3-MC) in a cell-free system, Hepa-1c1c7 cells, and liver of ICR mice.</p>\n </section>\n \n <section>\n \n <h3> Methods and results</h3>\n \n <p>In the cell-free system, cardamonin dose-dependently inhibits AhR transformation. Chalcones with substitution on 2′ and/or 6′ position is important for the suppressive effect, while the substitution on 4′ position is negatively for the effect. Moreover, cardamonin and 2′-hydroxychalcone competitively inhibit the binding of [<sup>3</sup>H]-3-MC to the AhR. In Hepa-1c1c7 cells, cardamonin inhibits AhR transformation and expression of cytochrome P4501A1 (CYP1A1) in a dose-dependent manner through suppressing TCDD-induced phosphorylation of both AhR and AhR nuclear translocator, heterodimerization of them, and nuclear translocation of AhR. In the liver of mice, oral administered cardamonin also inhibits 3-MC-induced AhR translocation and expression of CYP1A1.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>Among used chalcones, a natural chalcone cardamonin competitively binds to AhR and suppresses its transformation. Thus, cardamonin is an effective food factor for suppression of the dioxin-caused biochemical alterations and toxicities.</p>\n </section>\n </div>","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"68 18","pages":""},"PeriodicalIF":4.5000,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A Natural Chalcone Cardamonin Inhibited Transformation of Aryl Hydrocarbon Receptor Through Binding to the Receptor Competitively\",\"authors\":\"Tianshun Zhang,&nbsp;Chao He,&nbsp;Shieru Ota,&nbsp;Tomoya Kitakaze,&nbsp;Ryoichi Yamaji,&nbsp;Sayuri Shimazu,&nbsp;Yoko Yamashita,&nbsp;Hitoshi Ashida\",\"doi\":\"10.1002/mnfr.202400185\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <section>\\n \\n <h3> Scope</h3>\\n \\n <p>Chalcones are widely present in most plants and have various health beneficial functions. This study investigates the suppressive effect of 13 natural and synthetic chalcones on transformation of aryl hydrocarbon receptor (AhR) induced by 2,3,7,8-tetrachlorodibenzo-<i>p</i>-dioxin (TCDD) and 3-methylcholanthrene (3-MC) in a cell-free system, Hepa-1c1c7 cells, and liver of ICR mice.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods and results</h3>\\n \\n <p>In the cell-free system, cardamonin dose-dependently inhibits AhR transformation. Chalcones with substitution on 2′ and/or 6′ position is important for the suppressive effect, while the substitution on 4′ position is negatively for the effect. Moreover, cardamonin and 2′-hydroxychalcone competitively inhibit the binding of [<sup>3</sup>H]-3-MC to the AhR. In Hepa-1c1c7 cells, cardamonin inhibits AhR transformation and expression of cytochrome P4501A1 (CYP1A1) in a dose-dependent manner through suppressing TCDD-induced phosphorylation of both AhR and AhR nuclear translocator, heterodimerization of them, and nuclear translocation of AhR. In the liver of mice, oral administered cardamonin also inhibits 3-MC-induced AhR translocation and expression of CYP1A1.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusion</h3>\\n \\n <p>Among used chalcones, a natural chalcone cardamonin competitively binds to AhR and suppresses its transformation. Thus, cardamonin is an effective food factor for suppression of the dioxin-caused biochemical alterations and toxicities.</p>\\n </section>\\n </div>\",\"PeriodicalId\":212,\"journal\":{\"name\":\"Molecular Nutrition & Food Research\",\"volume\":\"68 18\",\"pages\":\"\"},\"PeriodicalIF\":4.5000,\"publicationDate\":\"2024-08-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Molecular Nutrition & Food Research\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/mnfr.202400185\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"FOOD SCIENCE & TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular Nutrition & Food Research","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/mnfr.202400185","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

范围查耳酮广泛存在于大多数植物中,具有多种有益健康的功能。本研究在无细胞系统、Hepa-1c1c7 细胞和 ICR 小鼠肝脏中研究了 13 种天然和合成查耳酮对 2,3,7,8- 四氯二苯并对二恶英(TCDD)和 3-甲基胆蒽(3-MC)诱导的芳基烃受体(AhR)转化的抑制作用:在无细胞系统中,白豆蔻素具有剂量依赖性地抑制 AhR 转化。在2'和/或6'位上取代的查耳酮对抑制作用起重要作用,而在4'位上取代的查耳酮对抑制作用起负面作用。此外,白豆蔻素和 2'- 羟基查尔酮还能竞争性地抑制 [3H]-3-MC 与 AhR 的结合。在 Hepa-1c1c7 细胞中,白豆蔻素通过抑制 TCDD 诱导的 AhR 和 AhR 核转运体的磷酸化、异二聚化以及 AhR 的核转运,以剂量依赖的方式抑制 AhR 转化和细胞色素 P4501A1(CYP1A1)的表达。在小鼠肝脏中,口服白豆蔻素还能抑制 3-MC 诱导的 AhR 转位和 CYP1A1 的表达:结论:在常用的查耳酮中,一种天然查耳酮--白豆蔻素能竞争性地与 AhR 结合并抑制其转化。因此,白豆蔻素是一种有效的食物因子,可抑制二恶英引起的生化改变和毒性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

A Natural Chalcone Cardamonin Inhibited Transformation of Aryl Hydrocarbon Receptor Through Binding to the Receptor Competitively

A Natural Chalcone Cardamonin Inhibited Transformation of Aryl Hydrocarbon Receptor Through Binding to the Receptor Competitively

Scope

Chalcones are widely present in most plants and have various health beneficial functions. This study investigates the suppressive effect of 13 natural and synthetic chalcones on transformation of aryl hydrocarbon receptor (AhR) induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and 3-methylcholanthrene (3-MC) in a cell-free system, Hepa-1c1c7 cells, and liver of ICR mice.

Methods and results

In the cell-free system, cardamonin dose-dependently inhibits AhR transformation. Chalcones with substitution on 2′ and/or 6′ position is important for the suppressive effect, while the substitution on 4′ position is negatively for the effect. Moreover, cardamonin and 2′-hydroxychalcone competitively inhibit the binding of [3H]-3-MC to the AhR. In Hepa-1c1c7 cells, cardamonin inhibits AhR transformation and expression of cytochrome P4501A1 (CYP1A1) in a dose-dependent manner through suppressing TCDD-induced phosphorylation of both AhR and AhR nuclear translocator, heterodimerization of them, and nuclear translocation of AhR. In the liver of mice, oral administered cardamonin also inhibits 3-MC-induced AhR translocation and expression of CYP1A1.

Conclusion

Among used chalcones, a natural chalcone cardamonin competitively binds to AhR and suppresses its transformation. Thus, cardamonin is an effective food factor for suppression of the dioxin-caused biochemical alterations and toxicities.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Molecular Nutrition & Food Research
Molecular Nutrition & Food Research 工程技术-食品科技
CiteScore
8.70
自引率
1.90%
发文量
250
审稿时长
1.7 months
期刊介绍: Molecular Nutrition & Food Research is a primary research journal devoted to health, safety and all aspects of molecular nutrition such as nutritional biochemistry, nutrigenomics and metabolomics aiming to link the information arising from related disciplines: Bioactivity: Nutritional and medical effects of food constituents including bioavailability and kinetics. Immunology: Understanding the interactions of food and the immune system. Microbiology: Food spoilage, food pathogens, chemical and physical approaches of fermented foods and novel microbial processes. Chemistry: Isolation and analysis of bioactive food ingredients while considering environmental aspects.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信