在超高效液相色谱/质谱四极杆飞行时间、高效液相色谱、高效液相色谱和核磁共振分析平台上对余甘子花进行了全面的植物化学分析,发现了在人类(THP-1)巨噬细胞中具有强效抗炎作用的功能代谢物。

IF 3 3区 生物学 Q2 BIOCHEMICAL RESEARCH METHODS
Acharya Balkrishna, Sudeep Verma, Priya Rani M, Monali Joshi, Meenu Tomer, Vivek Gohel, Pardeep Nain, Rishabh Dev, Anurag Varshney
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引用次数: 0

摘要

导言:大叶女贞(Phyllanthus emblica L.)因其果实和叶片的药理作用而闻名于世,受到了广泛关注。然而,对其花的研究,特别是代谢物分析和药理活性方面的研究却明显不足:本研究旨在通过超高效液相色谱-四极杆飞行时间质谱法(UHPLC-QToF-MS)、高效薄层色谱法(HPTLC)、高效液相色谱法(HPLC)、红外光谱法和核磁共振波谱法等方法,鉴定水乙醇提取物中的植物化学成分,并评估其抗炎潜力:采用超高效液相色谱/质谱-QToF正负离子模式鉴定和表征了余甘子花中的植物化学物质。此外,还使用 HPTLC、HPLC、FT-IR 和 NMR 方法分析了花提取物中的标记化合物。通过评估炎症生物标志物,评估了脂多糖刺激的 THP-1 巨噬细胞的抗炎潜力:结果:超高效液相色谱/质谱-QToF分析有助于根据没食子酸衍生物、黄酮苷和单宁酸的片段模式和以前的文献报道,从白花蛇舌草中鉴定出51种化合物。值得注意的是,该研究还首次在该物种中鉴定出了亚精胺化合物。对 HPTLC 和 HPLC 方法的优化表明,柯里拉京是主要化合物,其次是傅立叶变换红外光谱和核磁共振光谱方法。此外,用余甘子花的水乙醇提取物处理可降低促炎细胞因子 TNF-α、IL-1β 和 IL-6 的水平,同时调节脂多糖诱导的 THP-1 巨噬细胞中核因子-κB 的活性:结论:色谱技术与光谱方法相结合发现,在鉴定苦蘵花中存在的特征植物代谢物方面具有很强的普遍性,这为苦蘵花的抗炎潜力奠定了基础。这些研究为进一步探索白花蛇舌草在各个领域的潜在应用奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comprehensive phytochemical profiling of Phyllanthus emblica L. flowers on UHPLC/MS quadrupole time of flight, HPTLC, HPLC, and NMR analytical platforms reveals functional metabolites with potent anti-inflammatory effects in human (THP-1) macrophages.

Introduction: Phyllanthus emblica L., renowned for its pharmacological benefits found in its fruits and leaves, has received considerable attention. However, there is a notable lack of research on its flowers, specifically on metabolite profiling and pharmacological activity.

Objective: The present study aims to delineate the phytochemical constituents of hydromethanolic extract of P. emblica flowers by ultra-high-performance liquid chromatography coupled with quadrupole time of flight mass spectrometry (UHPLC-QToF-MS), high-performance thin layer chromatography (HPTLC), high-performance liquid chromatography (HPLC), infrared and nuclear magnetic resonance spectroscopic methods and subsequent evaluation of its anti-inflammatory potential.

Materials and methods: The identification and characterization of phytochemicals in P. emblica flowers was performed by UHPLC/MS-QToF in both positive and negative ionization modes. Additionally, marker compounds present in flower extract were analyzed using HPTLC, HPLC, FT-IR, and NMR methods. The anti-inflammatory potential was evaluated in lipopolysaccharide-stimulated THP-1 macrophages by evaluating inflammatory biomarkers.

Results: UHPLC/MS-QToF analysis facilitated the identification of 51 compounds from P. emblica flowers including gallic acid derivatives, flavonoid glycosides, and tannins based on their fragmentation patterns and previous literature reports. Notably, the study also identified spermidine compounds for the first time in this species. Optimization of HPTLC and HPLC methods marked the presence of corilagin as major compound followed by FT-IR and NMR spectral methods. Moreover, treatment with hydromethanolic extract of P. emblica flowers resulted in decreased levels of proinflammatory cytokines, TNF-α, IL-1β, and IL-6, alongside modulation of nuclear factor-κB activity in lipopolysaccharide-induced THP-1 macrophages.

Conclusion: Chromatographic techniques in conjunction with spectral methods found robust prevalence in the identification of signature phytometabolites present in P. emblica flowers, which sets the basis for its anti-inflammatory potentials. The studies established a foundation for further exploration of potential applications of P. emblica flowers across various domains.

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来源期刊
Phytochemical Analysis
Phytochemical Analysis 生物-分析化学
CiteScore
6.00
自引率
6.10%
发文量
88
审稿时长
1.7 months
期刊介绍: Phytochemical Analysis is devoted to the publication of original articles concerning the development, improvement, validation and/or extension of application of analytical methodology in the plant sciences. The spectrum of coverage is broad, encompassing methods and techniques relevant to the detection (including bio-screening), extraction, separation, purification, identification and quantification of compounds in plant biochemistry, plant cellular and molecular biology, plant biotechnology, the food sciences, agriculture and horticulture. The Journal publishes papers describing significant novelty in the analysis of whole plants (including algae), plant cells, tissues and organs, plant-derived extracts and plant products (including those which have been partially or completely refined for use in the food, agrochemical, pharmaceutical and related industries). All forms of physical, chemical, biochemical, spectroscopic, radiometric, electrometric, chromatographic, metabolomic and chemometric investigations of plant products (monomeric species as well as polymeric molecules such as nucleic acids, proteins, lipids and carbohydrates) are included within the remit of the Journal. Papers dealing with novel methods relating to areas such as data handling/ data mining in plant sciences will also be welcomed.
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