{"title":"枯草芽孢杆菌 YC89 介导的根瘤菌招募提高了甘蔗红腐病的抗性","authors":"Linyan Xie, Lufeng Liu, Yanju Luo, Xibing Rao, Shaozhi LV, Zhenfeng Qian, Yining Di, Hongbo Lou, Lilian He, Fusheng Li","doi":"10.1186/s40538-024-00627-4","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Sugarcane red rot is a soil-borne disease caused by <i>Colletotrichum falcatum</i>. It can reduce the yield of sugarcane and the purity of sugarcane juice, which seriously restricts the development of sucrose industry. Biocontrol bacteria can control diseases by regulating rhizosphere microecology. In this study, the effects of biocontrol bacteria on sugarcane rhizosphere microecology were studied by metagenomics and metabolomics, and the control effects of biocontrol bacteria and rhizosphere dominant bacteria on sugarcane red rot were further explored by pot experiment.</p><h3>Results</h3><p>The results of metagenomic sequencing showed that inoculation with <i>B. velezensis</i> YC89 and pathogens could significantly change the microbial diversity of the sugarcane rhizosphere. The relative abundance of beneficial strains such as <i>Streptomyces</i>, <i>Burkholderia</i>, <i>Sphingomonas</i>, and <i>Rhizobium</i> increased significantly in the rhizosphere of sugarcane in the YC treatment group. <i>Pseudomonas</i> was significantly enriched in the rhizosphere of sugarcane in the C treatment group. The results of metabolome sequencing showed that the content of amino acids in sugarcane root exudates increased after inoculation with <i>B. velezensis</i> YC89, and the contents of phenolic acids and flavonoids decreased. Spearman correlation analysis showed that there was a significant correlation between differential metabolites and rhizosphere microorganisms. The results of pot experiment showed that YC89 strain and three rhizosphere microorganisms could significantly reduce the disease index of red rot and promote the growth of sugarcane plants. In addition, these strains can also significantly increase the JA and SA content of sugarcane leaves and induce plant system resistance-related enzyme activities. Among them, the synthetic community treatment group had the best biocontrol effect on red rot, and its relative control effect was 67.50%.</p><h3>Conclusions</h3><p>Therefore, we conclude that <i>B. velezensis</i> YC89 could recruit beneficial rhizosphere microorganisms to enrich the rhizosphere and change the content of some phenolic acids and flavonoids in the root exudates. In addition, the isolated rhizosphere dominant bacteria and YC89 strain can resist red rot by inducing plant systemic resistance and promote the growth of sugarcane plants. This study provides a theoretical basis for the use of biocontrol bacteria to regulate rhizosphere bacteria to jointly control plant soil-borne diseases.</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":512,"journal":{"name":"Chemical and Biological Technologies in Agriculture","volume":"11 1","pages":""},"PeriodicalIF":5.2000,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://chembioagro.springeropen.com/counter/pdf/10.1186/s40538-024-00627-4","citationCount":"0","resultStr":"{\"title\":\"Bacillus velezensis YC89-mediated recruitment of rhizosphere bacteria improves resistance against sugarcane red rot\",\"authors\":\"Linyan Xie, Lufeng Liu, Yanju Luo, Xibing Rao, Shaozhi LV, Zhenfeng Qian, Yining Di, Hongbo Lou, Lilian He, Fusheng Li\",\"doi\":\"10.1186/s40538-024-00627-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Sugarcane red rot is a soil-borne disease caused by <i>Colletotrichum falcatum</i>. It can reduce the yield of sugarcane and the purity of sugarcane juice, which seriously restricts the development of sucrose industry. Biocontrol bacteria can control diseases by regulating rhizosphere microecology. In this study, the effects of biocontrol bacteria on sugarcane rhizosphere microecology were studied by metagenomics and metabolomics, and the control effects of biocontrol bacteria and rhizosphere dominant bacteria on sugarcane red rot were further explored by pot experiment.</p><h3>Results</h3><p>The results of metagenomic sequencing showed that inoculation with <i>B. velezensis</i> YC89 and pathogens could significantly change the microbial diversity of the sugarcane rhizosphere. The relative abundance of beneficial strains such as <i>Streptomyces</i>, <i>Burkholderia</i>, <i>Sphingomonas</i>, and <i>Rhizobium</i> increased significantly in the rhizosphere of sugarcane in the YC treatment group. <i>Pseudomonas</i> was significantly enriched in the rhizosphere of sugarcane in the C treatment group. The results of metabolome sequencing showed that the content of amino acids in sugarcane root exudates increased after inoculation with <i>B. velezensis</i> YC89, and the contents of phenolic acids and flavonoids decreased. Spearman correlation analysis showed that there was a significant correlation between differential metabolites and rhizosphere microorganisms. The results of pot experiment showed that YC89 strain and three rhizosphere microorganisms could significantly reduce the disease index of red rot and promote the growth of sugarcane plants. In addition, these strains can also significantly increase the JA and SA content of sugarcane leaves and induce plant system resistance-related enzyme activities. Among them, the synthetic community treatment group had the best biocontrol effect on red rot, and its relative control effect was 67.50%.</p><h3>Conclusions</h3><p>Therefore, we conclude that <i>B. velezensis</i> YC89 could recruit beneficial rhizosphere microorganisms to enrich the rhizosphere and change the content of some phenolic acids and flavonoids in the root exudates. In addition, the isolated rhizosphere dominant bacteria and YC89 strain can resist red rot by inducing plant systemic resistance and promote the growth of sugarcane plants. 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引用次数: 0
摘要
背景甘蔗红腐病是由镰刀菌（Colletotrichum falcatum）引起的土传病害。它能降低甘蔗产量和蔗汁纯度，严重制约蔗糖产业的发展。生物防治菌可以通过调节根瘤菌的微生态来控制病害。本研究通过元基因组学和代谢组学研究了生防菌对甘蔗根瘤菌群微生态的影响，并通过盆栽实验进一步探讨了生防菌和根瘤菌群优势菌对甘蔗红腐病的防治效果。结果元基因组测序结果表明，接种B. velezensis YC89和病原菌可显著改变甘蔗根瘤菌群的微生物多样性。YC 处理组甘蔗根瘤菌层中有益菌株（如链霉菌、伯克霍尔德氏菌、鞘氨单胞菌和根瘤菌）的相对丰度显著增加。假单胞菌在 C 处理组甘蔗根圈中明显富集。代谢组测序结果表明，接种 B. velezensis YC89 后，甘蔗根渗出液中氨基酸含量增加，酚酸和类黄酮含量减少。斯皮尔曼相关分析表明，差异代谢产物与根瘤微生物之间存在显著相关。盆栽实验结果表明，YC89 菌株和三种根瘤微生物能显著降低红腐病的病害指数，促进甘蔗植株的生长。此外，这些菌株还能显著提高甘蔗叶片的 JA 和 SA 含量，诱导植物系统抗性相关酶的活性。结论因此，我们得出结论：B. velezensis YC89 可以招募有益的根圈微生物来丰富根圈，并改变根渗出液中一些酚酸和类黄酮的含量。此外，分离的根圈优势菌和 YC89 菌株可通过诱导植物系统抗性来抵抗红腐病，促进甘蔗植株的生长。该研究为利用生防菌调控根瘤菌共同防治植物土传病害提供了理论依据。 图文摘要
Bacillus velezensis YC89-mediated recruitment of rhizosphere bacteria improves resistance against sugarcane red rot
Background
Sugarcane red rot is a soil-borne disease caused by Colletotrichum falcatum. It can reduce the yield of sugarcane and the purity of sugarcane juice, which seriously restricts the development of sucrose industry. Biocontrol bacteria can control diseases by regulating rhizosphere microecology. In this study, the effects of biocontrol bacteria on sugarcane rhizosphere microecology were studied by metagenomics and metabolomics, and the control effects of biocontrol bacteria and rhizosphere dominant bacteria on sugarcane red rot were further explored by pot experiment.
Results
The results of metagenomic sequencing showed that inoculation with B. velezensis YC89 and pathogens could significantly change the microbial diversity of the sugarcane rhizosphere. The relative abundance of beneficial strains such as Streptomyces, Burkholderia, Sphingomonas, and Rhizobium increased significantly in the rhizosphere of sugarcane in the YC treatment group. Pseudomonas was significantly enriched in the rhizosphere of sugarcane in the C treatment group. The results of metabolome sequencing showed that the content of amino acids in sugarcane root exudates increased after inoculation with B. velezensis YC89, and the contents of phenolic acids and flavonoids decreased. Spearman correlation analysis showed that there was a significant correlation between differential metabolites and rhizosphere microorganisms. The results of pot experiment showed that YC89 strain and three rhizosphere microorganisms could significantly reduce the disease index of red rot and promote the growth of sugarcane plants. In addition, these strains can also significantly increase the JA and SA content of sugarcane leaves and induce plant system resistance-related enzyme activities. Among them, the synthetic community treatment group had the best biocontrol effect on red rot, and its relative control effect was 67.50%.
Conclusions
Therefore, we conclude that B. velezensis YC89 could recruit beneficial rhizosphere microorganisms to enrich the rhizosphere and change the content of some phenolic acids and flavonoids in the root exudates. In addition, the isolated rhizosphere dominant bacteria and YC89 strain can resist red rot by inducing plant systemic resistance and promote the growth of sugarcane plants. This study provides a theoretical basis for the use of biocontrol bacteria to regulate rhizosphere bacteria to jointly control plant soil-borne diseases.
期刊介绍：
Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture.
This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population.
Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.
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