人参皂苷 Rg1 通过调节 Pink1/ Parkin 介导的线粒体自噬和抑制小胶质细胞 NLRP3 激活改善脑缺血再灌注损伤

IF 3.5 3区 医学 Q2 NEUROSCIENCES
Changbai Sui , Ying Liu , Jun Jiang , Jianhua Tang , Ling Yu , Guoying Lv
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引用次数: 0

摘要

目的 本研究旨在进一步阐明人参皂苷Rg1治疗脑缺血再灌注的机制。方法 在本研究中,我们观察了Rg1给药前后氧-葡萄糖剥夺/再氧合(OGD/R)模型后RM细胞(小胶质细胞)的凋亡情况、线粒体膜电位的变化、活性氧(ROS)和炎性囊泡NLR Family Pyrin Domain Containing 3(NLRP3)含量的变化,以及自噬相关蛋白、炎性因子和凋亡蛋白的表达水平。我们在体内实验中进一步检测了 Rg1 给药前后 CI/RI 大鼠脑组织的病理形态学变化、神经元损伤、线粒体形态和线粒体结构的变化以及自噬相关蛋白、炎症因子和凋亡蛋白的表达水平。结果体外实验表明,Rg1通过调节PTEN诱导的推定激酶1(Pink1)/Parkin信号通路,诱导线粒体自噬,降低线粒体膜电位,减少ROS含量,从而抑制NLRP3活化,减少炎症因子分泌和RM细胞凋亡。体内实验表明,Rg1通过调节Pink1/Parkin信号通路,诱导线粒体自噬,抑制NLRP3活化,改善炎症反应,减少细胞凋亡;Rg1能显著缩小脑梗死面积,改善脑组织病理状态,减轻神经元损伤,从而改善大鼠脑缺血再灌注损伤。结论我们的研究结果表明,人参皂苷 Rg1 可通过调节小胶质细胞中 Pink1/ Parkin 介导的线粒体自噬和抑制小胶质细胞 NLRP3 激活来改善脑缺血再灌注损伤。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Ginsenoside Rg1 ameliorates cerebral ischemia-reperfusion injury by regulating Pink1/ Parkin-mediated mitochondrial autophagy and inhibiting microglia NLRP3 activation

Objective

This study aimed to further elucidate the mechanism of ginsenoside Rg1 in the treatment of cerebral ischemia-reperfusion.

Methods

In this study, we observed the apoptosis of RM cells (microglia) after oxygen-glucose deprivation/reoxygenation (OGD/R) modeling before and after Rg1 administration, changes in mitochondrial membrane potential, changes in the content of Reactive oxygen species (ROS) and inflammatory vesicles NLR Family Pyrin Domain Containing 3 (NLRP3), and the expression levels of autophagy-related proteins, inflammatory factors, and apoptosis proteins. We further examined the pathomorphological changes in brain tissue, neuronal damage, changes in mitochondrial morphology and mitochondrial structure, and the autophagy-related proteins, inflammatory factors, and apoptosis proteins expression levels in CI/RI rats before and after administration of Rg1 in vivo experiments.

Results

In vitro experiments showed that Rg1 induced mitochondrial autophagy, decreased mitochondrial membrane potential, and reduced ROS content thereby inhibiting NLRP3 activation, decreasing secretion of inflammatory factors and RM cell apoptosis by regulating the PTEN induced putative kinase 1(Pink1) /Parkin signaling pathway. In vivo experiments showed that Rg1 induced mitochondrial autophagy, inhibited NLRP3 activation, improved inflammatory response, and reduced apoptosis by regulating the Pink1/Parkin signaling pathway, and Rg1 significantly reduced the area of cerebral infarcts, improved the pathological state of brain tissue, and attenuated the neuronal damage, thus improving cerebral ischemia/reperfusion injury in rats.

Conclusion

Our results suggest that ginsenoside Rg1 can ameliorate cerebral ischemia-reperfusion injury by modulating Pink1/ Parkin-mediated mitochondrial autophagy in microglia and inhibiting microglial NLRP3 activation.

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来源期刊
Brain Research Bulletin
Brain Research Bulletin 医学-神经科学
CiteScore
6.90
自引率
2.60%
发文量
253
审稿时长
67 days
期刊介绍: The Brain Research Bulletin (BRB) aims to publish novel work that advances our knowledge of molecular and cellular mechanisms that underlie neural network properties associated with behavior, cognition and other brain functions during neurodevelopment and in the adult. Although clinical research is out of the Journal''s scope, the BRB also aims to publish translation research that provides insight into biological mechanisms and processes associated with neurodegeneration mechanisms, neurological diseases and neuropsychiatric disorders. The Journal is especially interested in research using novel methodologies, such as optogenetics, multielectrode array recordings and life imaging in wild-type and genetically-modified animal models, with the goal to advance our understanding of how neurons, glia and networks function in vivo.
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